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The map label for this gene is chiA [H]

Identifier: 34496895

GI number: 34496895

Start: 1522798

End: 1524150

Strand: Reverse

Name: chiA [H]

Synonym: CV_1440

Alternate gene names: 34496895

Gene position: 1524150-1522798 (Counterclockwise)

Preceding gene: 34496896

Following gene: 34496890

Centisome position: 32.08

GC content: 65.48

Gene sequence:

>1353_bases
ATGATGAAAAGGTTTGCCGCCCTCTCCGCTCTAGCCGCCGCCGCGGCCGGTCTCGCCAGTCCCGCCGCCCAGGCCGGCGC
CTTCGCGCCCTATGTCGACATGACCTTGTGGCCGACGCCCCAAATCGACAAGCTGGGCGTCAATCAAGGCATCCAGCAAT
TCACCCTCGCCTTCGTCGTCGCCAAGGGAGGCTGCGCGCCCTCCTGGGGCGGCGTGCTGGCCATCCCGGGCAGCGGTTCC
GACCAGCAACTGAGCGCCATCCGCAACGGCATCAATAATTTCCGCGGCAAAGGCGGCGAAGTGATGGTGTCCTTCGGCGG
CGCCAACGGCACGCCGCTGCAACAGGCCTGCACCACCAACGCCAGCCTGCAGGCCGCCTACCAGACCGTGCTGGACACTT
ACAACCTGTCGCGCATCGATTTCGACATCGAAGGCGGCGCGCAAACCGATACCGCCGCCAACAACCGCAACTTCGCGGTG
GTGGCCGCGCTGCAGAAGAACTACAAGGCAAAGGGCAAGACGCTGCATGTATCGCTGACCCTGCCGGCCATGCCTTTCGG
CCTGACCCAGGATGGCCAGCGCGTGCTCGCGTCCGCGCTCGCCAACGGCGTGGCGCTGGACACCGTCAACATCATGGCGA
TGGACTACGGCCAGTCCAATCCGAACATGGGCGCCGCCGCCAAGCAGGCCGCCCAGGCGCTGTACAGCCAGATCGATGCC
GCCTACAAGGCCCATGGCCAGACGCTGACCGACGCCCAGCTGTGGCAAAAGGTGGGCGTCACGCCCATGGTGGGCCTGAA
CGACACCCAGCCGGAAACCTTCACCGTGGACAACGCCAAGGACCTGTACGGCATGGCCAACAGCAATCGCTTCGGGCTGT
TGTCGATGTGGTCGATCAGCCGCGACAAGTCTTGCCCCAATAACGGCCATTACGTCGACGCGCAATGCTCCGGCATCGTG
CAAACGCCTTACGCCTTCAGCAATGTGTTCAAGGGCTTCAAGGACCACTGGGGCAGCGGCGTCACCCAGGACCCGAATTA
CGGCGGCGGCGACAACGGCAATGGCGGCGGGCCGGTCAACGGCCAGCCATGGTCGTCGGGCCAGGTCTACAACACCGGCA
ACACCGTCACCTACTCCGGCGCCACCTGGAAAGCCCAGTGGTGGACCCAGGGCGATGTGCCCGGCCAGGCTTCGGTGTGG
CAGCAGCAGGGCGGCGGCTTGCAGCAATGGTCGGCGACCGCCGCCTATTCCGCCAAGGACTGCGCGCTGTACCAAGGCAA
GAAGTACTGCGCCAAATGGTGGACCCAGGGCAATCTGCCCGCCGCCGGCGACCCGTGGGTGCTGTCCAACTGA

Upstream 100 bases:

>100_bases
ATATTTAGCAATTGCTGACAAATGTTGGCGTTTTTATAAACCATTTAATATGCTCAGCCATTCGCGACACCAGAATGCTG
CGCAGCCCGGGAGTCCGCTC

Downstream 100 bases:

>100_bases
GCGGACGCTCTCCGCAAAAGCCAAGCCGGGCATGCGCCCGGCTTTTTTGAATCCTGCCGATCTAGCCCGGCATATCGGGC
TCCACCAGCGCCGCCAGCTG

Product: hydrolase transmembrane protein

Products: NA

Alternate protein names: Chitinase; Lysozyme [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MMKRFAALSALAAAAAGLASPAAQAGAFAPYVDMTLWPTPQIDKLGVNQGIQQFTLAFVVAKGGCAPSWGGVLAIPGSGS
DQQLSAIRNGINNFRGKGGEVMVSFGGANGTPLQQACTTNASLQAAYQTVLDTYNLSRIDFDIEGGAQTDTAANNRNFAV
VAALQKNYKAKGKTLHVSLTLPAMPFGLTQDGQRVLASALANGVALDTVNIMAMDYGQSNPNMGAAAKQAAQALYSQIDA
AYKAHGQTLTDAQLWQKVGVTPMVGLNDTQPETFTVDNAKDLYGMANSNRFGLLSMWSISRDKSCPNNGHYVDAQCSGIV
QTPYAFSNVFKGFKDHWGSGVTQDPNYGGGDNGNGGGPVNGQPWSSGQVYNTGNTVTYSGATWKAQWWTQGDVPGQASVW
QQQGGGLQQWSATAAYSAKDCALYQGKKYCAKWWTQGNLPAAGDPWVLSN

Sequences:

>Translated_450_residues
MMKRFAALSALAAAAAGLASPAAQAGAFAPYVDMTLWPTPQIDKLGVNQGIQQFTLAFVVAKGGCAPSWGGVLAIPGSGS
DQQLSAIRNGINNFRGKGGEVMVSFGGANGTPLQQACTTNASLQAAYQTVLDTYNLSRIDFDIEGGAQTDTAANNRNFAV
VAALQKNYKAKGKTLHVSLTLPAMPFGLTQDGQRVLASALANGVALDTVNIMAMDYGQSNPNMGAAAKQAAQALYSQIDA
AYKAHGQTLTDAQLWQKVGVTPMVGLNDTQPETFTVDNAKDLYGMANSNRFGLLSMWSISRDKSCPNNGHYVDAQCSGIV
QTPYAFSNVFKGFKDHWGSGVTQDPNYGGGDNGNGGGPVNGQPWSSGQVYNTGNTVTYSGATWKAQWWTQGDVPGQASVW
QQQGGGLQQWSATAAYSAKDCALYQGKKYCAKWWTQGNLPAAGDPWVLSN
>Mature_450_residues
MMKRFAALSALAAAAAGLASPAAQAGAFAPYVDMTLWPTPQIDKLGVNQGIQQFTLAFVVAKGGCAPSWGGVLAIPGSGS
DQQLSAIRNGINNFRGKGGEVMVSFGGANGTPLQQACTTNASLQAAYQTVLDTYNLSRIDFDIEGGAQTDTAANNRNFAV
VAALQKNYKAKGKTLHVSLTLPAMPFGLTQDGQRVLASALANGVALDTVNIMAMDYGQSNPNMGAAAKQAAQALYSQIDA
AYKAHGQTLTDAQLWQKVGVTPMVGLNDTQPETFTVDNAKDLYGMANSNRFGLLSMWSISRDKSCPNNGHYVDAQCSGIV
QTPYAFSNVFKGFKDHWGSGVTQDPNYGGGDNGNGGGPVNGQPWSSGQVYNTGNTVTYSGATWKAQWWTQGDVPGQASVW
QQQGGGLQQWSATAAYSAKDCALYQGKKYCAKWWTQGNLPAAGDPWVLSN

Specific function: Bifunctional enzyme with lysozyme/chitinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 chitin-binding type-3 domains [H]

Homologues:

Organism=Escherichia coli, GI1789736, Length=320, Percent_Identity=35.9375, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003610
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02839 CBM_5_12 [H]

EC number: =3.2.1.14; =3.2.1.17 [H]

Molecular weight: Translated: 47375; Mature: 47375

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRFAALSALAAAAAGLASPAAQAGAFAPYVDMTLWPTPQIDKLGVNQGIQQFTLAFVV
CCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEECEEECCCCCCCCCCHHHHHHHHHHHHEE
AKGGCAPSWGGVLAIPGSGSDQQLSAIRNGINNFRGKGGEVMVSFGGANGTPLQQACTTN
ECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCC
ASLQAAYQTVLDTYNLSRIDFDIEGGAQTDTAANNRNFAVVAALQKNYKAKGKTLHVSLT
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEHHCCCCCCCEEEEEEE
LPAMPFGLTQDGQRVLASALANGVALDTVNIMAMDYGQSNPNMGAAAKQAAQALYSQIDA
CCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
AYKAHGQTLTDAQLWQKVGVTPMVGLNDTQPETFTVDNAKDLYGMANSNRFGLLSMWSIS
HHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEECCHHHHHCCCCCCCEEEEEEEECC
RDKSCPNNGHYVDAQCSGIVQTPYAFSNVFKGFKDHWGSGVTQDPNYGGGDNGNGGGPVN
CCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GQPWSSGQVYNTGNTVTYSGATWKAQWWTQGDVPGQASVWQQQGGGLQQWSATAAYSAKD
CCCCCCCEEEECCCEEEECCCEEEEECEECCCCCCCHHHHHHCCCCHHHHCHHHCCCCCH
CALYQGKKYCAKWWTQGNLPAAGDPWVLSN
HHHHCCHHHHHHHHCCCCCCCCCCCCEECC
>Mature Secondary Structure
MMKRFAALSALAAAAAGLASPAAQAGAFAPYVDMTLWPTPQIDKLGVNQGIQQFTLAFVV
CCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEECEEECCCCCCCCCCHHHHHHHHHHHHEE
AKGGCAPSWGGVLAIPGSGSDQQLSAIRNGINNFRGKGGEVMVSFGGANGTPLQQACTTN
ECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCC
ASLQAAYQTVLDTYNLSRIDFDIEGGAQTDTAANNRNFAVVAALQKNYKAKGKTLHVSLT
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEHHCCCCCCCEEEEEEE
LPAMPFGLTQDGQRVLASALANGVALDTVNIMAMDYGQSNPNMGAAAKQAAQALYSQIDA
CCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
AYKAHGQTLTDAQLWQKVGVTPMVGLNDTQPETFTVDNAKDLYGMANSNRFGLLSMWSIS
HHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEECCHHHHHCCCCCCCEEEEEEEECC
RDKSCPNNGHYVDAQCSGIVQTPYAFSNVFKGFKDHWGSGVTQDPNYGGGDNGNGGGPVN
CCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GQPWSSGQVYNTGNTVTYSGATWKAQWWTQGDVPGQASVWQQQGGGLQQWSATAAYSAKD
CCCCCCCEEEECCCEEEECCCEEEEECEECCCCCCCHHHHHHCCCCHHHHCHHHCCCCCH
CALYQGKKYCAKWWTQGNLPAAGDPWVLSN
HHHHCCHHHHHHHHCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2661540; 10419961; 10760150 [H]