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The map label for this gene is ygfZ [C]

Identifier: 34496817

GI number: 34496817

Start: 1433746

End: 1434780

Strand: Direct

Name: ygfZ [C]

Synonym: CV_1362

Alternate gene names: 34496817

Gene position: 1433746-1434780 (Clockwise)

Preceding gene: 34496813

Following gene: 34496818

Centisome position: 30.18

GC content: 57.49

Gene sequence:

>1035_bases
ATGAATGTATTCTGGGATGAATGGCTGAGACAAAAAGGCGTGGTCCGGAACGCAAACGGCCAAGCTCCGCTGGCATCGCA
TCTTAAACAGATCGCGGCCCTGGAAAATGGATCGGTATTGGCGCCCCTGGATAACTTCGCCCTGATCCGCGTCGAGGGCG
AGGACGCGGCCGCTTTTCTGCAGGGGCAGTTGTCGAATGATATCCGCGAGGTGACGACGGAGCGCGCGCAGTACAGCACC
TACTCCACTGCCAAGGGACGCATGCTGGCCAGTTTCCTGATCTGGCTGCGTGACGGAGCCTATTATCTGATGGTTTCCGC
GGATATCGCCGAAACCGTAGCCAAAAGGCTGACGATGTTTGTGCTGCGCTCCAAGGTGAAGGTGGTGTTGGACCGCGAAT
GGTCTTTATTGGGCGTGTCCGGTATTGCGATCGAGCAGGCGTTGCACAAACATTTCCCGGGGGCGGCCGGGGCGGAGGAA
ATGCGGGTTGCGTTCCAATCCGAGGGCATTCTGCTCGCCTTGCCGAGTGGGGGCTATCTGCTGGCTGAGCGCGATGGCGG
CGGCATAGGAAAGGATCTCGCGCAAATGGAGGGCCTGGAGGCCGCGCTGCCTGAGGCTTGGGCATGGAAGGATATTCAGG
CGGGAATCGCCTGGGTGACACAGGCTACGCAGGAACAGTTTGTGCCGCAAATGGCGAATATGGAATTGATTGGAGCGGTC
AACTTCAAGAAGGGCTGCTATCCGGGCCAGGAAATCGTCGCGCGCAGCCAGTACTTGGGCAAGATGAAGCGCCGGATGTT
CAAAGTATCTTTTGATGCGGCTCTGCCTGTCGGAGCGAAACTTTATAGTCCGCAATTGCCGGACCAGTCTATAGGCATGC
TGGCATCGGAATGCCGAGTGGGAGAGAACGCTTATCTGGGGCTGGCGGTTGCGCAATCACAAACCTGGGAGGCTGGCATA
TTTGCCGATGAGGGGCATACTATTGCGTTGCGCCGACTGGAATTGCCGTATCCAATCGAATCCGTAACGGAATAA

Upstream 100 bases:

>100_bases
TATGCAATGGTATTTCATTCCGCATGCCGGGCGGCGCTGGCGACGAAGCGGCCGTTGGCGGTAAGATCGGCCATCGAAAT
CAATGCGGAGTCGACGAAAC

Downstream 100 bases:

>100_bases
TGGGTAATCCGTTTTCTTTTTAATCCTTGACTTGTGCTATTTCGATTGGCTACATTACATCCTGTAAATCGTTAAACCAA
TAATCAAGGGAACATGTTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNVFWDEWLRQKGVVRNANGQAPLASHLKQIAALENGSVLAPLDNFALIRVEGEDAAAFLQGQLSNDIREVTTERAQYST
YSTAKGRMLASFLIWLRDGAYYLMVSADIAETVAKRLTMFVLRSKVKVVLDREWSLLGVSGIAIEQALHKHFPGAAGAEE
MRVAFQSEGILLALPSGGYLLAERDGGGIGKDLAQMEGLEAALPEAWAWKDIQAGIAWVTQATQEQFVPQMANMELIGAV
NFKKGCYPGQEIVARSQYLGKMKRRMFKVSFDAALPVGAKLYSPQLPDQSIGMLASECRVGENAYLGLAVAQSQTWEAGI
FADEGHTIALRRLELPYPIESVTE

Sequences:

>Translated_344_residues
MNVFWDEWLRQKGVVRNANGQAPLASHLKQIAALENGSVLAPLDNFALIRVEGEDAAAFLQGQLSNDIREVTTERAQYST
YSTAKGRMLASFLIWLRDGAYYLMVSADIAETVAKRLTMFVLRSKVKVVLDREWSLLGVSGIAIEQALHKHFPGAAGAEE
MRVAFQSEGILLALPSGGYLLAERDGGGIGKDLAQMEGLEAALPEAWAWKDIQAGIAWVTQATQEQFVPQMANMELIGAV
NFKKGCYPGQEIVARSQYLGKMKRRMFKVSFDAALPVGAKLYSPQLPDQSIGMLASECRVGENAYLGLAVAQSQTWEAGI
FADEGHTIALRRLELPYPIESVTE
>Mature_344_residues
MNVFWDEWLRQKGVVRNANGQAPLASHLKQIAALENGSVLAPLDNFALIRVEGEDAAAFLQGQLSNDIREVTTERAQYST
YSTAKGRMLASFLIWLRDGAYYLMVSADIAETVAKRLTMFVLRSKVKVVLDREWSLLGVSGIAIEQALHKHFPGAAGAEE
MRVAFQSEGILLALPSGGYLLAERDGGGIGKDLAQMEGLEAALPEAWAWKDIQAGIAWVTQATQEQFVPQMANMELIGAV
NFKKGCYPGQEIVARSQYLGKMKRRMFKVSFDAALPVGAKLYSPQLPDQSIGMLASECRVGENAYLGLAVAQSQTWEAGI
FADEGHTIALRRLELPYPIESVTE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Homo sapiens, GI58197556, Length=263, Percent_Identity=27.3764258555133, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789265, Length=233, Percent_Identity=29.6137339055794, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 37698; Mature: 37698

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFWDEWLRQKGVVRNANGQAPLASHLKQIAALENGSVLAPLDNFALIRVEGEDAAAFL
CCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCHHHHH
QGQLSNDIREVTTERAQYSTYSTAKGRMLASFLIWLRDGAYYLMVSADIAETVAKRLTMF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHH
VLRSKVKVVLDREWSLLGVSGIAIEQALHKHFPGAAGAEEMRVAFQSEGILLALPSGGYL
HHHHHHHEEEECCCCEEECCHHHHHHHHHHHCCCCCCHHHHHHEEECCCEEEEECCCCEE
LAERDGGGIGKDLAQMEGLEAALPEAWAWKDIQAGIAWVTQATQEQFVPQMANMELIGAV
EEECCCCCCCHHHHHHCCHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
NFKKGCYPGQEIVARSQYLGKMKRRMFKVSFDAALPVGAKLYSPQLPDQSIGMLASECRV
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
GENAYLGLAVAQSQTWEAGIFADEGHTIALRRLELPYPIESVTE
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCCHHHHCC
>Mature Secondary Structure
MNVFWDEWLRQKGVVRNANGQAPLASHLKQIAALENGSVLAPLDNFALIRVEGEDAAAFL
CCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCHHHHH
QGQLSNDIREVTTERAQYSTYSTAKGRMLASFLIWLRDGAYYLMVSADIAETVAKRLTMF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHH
VLRSKVKVVLDREWSLLGVSGIAIEQALHKHFPGAAGAEEMRVAFQSEGILLALPSGGYL
HHHHHHHEEEECCCCEEECCHHHHHHHHHHHCCCCCCHHHHHHEEECCCEEEEECCCCEE
LAERDGGGIGKDLAQMEGLEAALPEAWAWKDIQAGIAWVTQATQEQFVPQMANMELIGAV
EEECCCCCCCHHHHHHCCHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
NFKKGCYPGQEIVARSQYLGKMKRRMFKVSFDAALPVGAKLYSPQLPDQSIGMLASECRV
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
GENAYLGLAVAQSQTWEAGIFADEGHTIALRRLELPYPIESVTE
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]