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The map label for this gene is pheS

Identifier: 34496807

GI number: 34496807

Start: 1423670

End: 1424656

Strand: Direct

Name: pheS

Synonym: CV_1352

Alternate gene names: 34496807

Gene position: 1423670-1424656 (Clockwise)

Preceding gene: 34496806

Following gene: 34496808

Centisome position: 29.97

GC content: 64.13

Gene sequence:

>987_bases
ATGAACAACGTTGACGCGATTCTCCAAGCCGGCCAGGCGGCAGTTGCCGCCGCGGCCGATCTGATCGAGCTCGAGCAGGT
CAAGGCGCGCTTCCTTGGCAAGAGCGGCGAGCTGACCGAACTCCTGAAGCAGCTGGGCAAGCTGGCGCCGGAAGAGCGCA
AGGCCGCCGGCGCCACCATCAACCAGGCCAAGCAGGCGTTCGAAGCCGCGCACAACGACAAGCGCGACCAGCTCAACGCC
GCCAAGCTGGAAGCCCAACTGGCCGCCGAGGCGCTGGACGTCACGCTGCCGGGCCGCGGTTCGGTCGGCGGCGGCCTGCA
TCCGGTGGCGCTGACGCTGGAGCGCATCGCCGGCCTGTTCCGCACCATGGGCTTCGAAGTGGCCGACGGCCCGGAAATCG
AAAGCGATTTCTACAACTTCCAGGCGCTGAACATTCCGGAAAATCATCCGGCGCGCGCGATGCAGGACACGTTCTACGTG
GAAAACTGCGACGTGCTGCGCACCCATACCAGCCCGATCCAGGCGCGCTTCATGGAAAGCCACCAGCCGCCGATCAAGAT
CGTCGCGCCGGGCCGCGTGTATCGCGTCGACTCCGACGCCACCCATTCGCCGATGTTCCATCAGATGGAGGGCCTGTGGG
TGGAGGAGGGCGTGTCCTTCGCCGATCTGAAGGCCGTGGTCACCGATTTCCTGCGCCGCTTCTTCGAGCGCGACGATCTG
CAGGTGCGCTTCCGCCCGTCCTTCTTCCCGTTCACCGAGACTTCGGCCGAGATCGACGTGCTGGACGAGCAGGGCAAATG
GCTGGAAGTGGGCGGTTGCGGCATGGTGCACCCGAAAGTGCTGAGCATCGCCAACATCGACCCGGAAAAATACACCGGCT
TCGCCTTCGGCATCGGCCTGGATCGTTTCGCCATGCTGCGCTACGGCGTCAACGACCTGCGCCTGTTCTTCGAGAACGAT
CTCAACTTCCTGAAGCAATTCAACTGA

Upstream 100 bases:

>100_bases
TTTTTTTCTATTCAGGCCGCCTTTCCAGAGCGTTGATCAGAGCGGGTATGCGCAATGCCCGATGTGATCAGCCTTCTATT
CGCATACGGTGTGGGACACG

Downstream 100 bases:

>100_bases
TCGCGCACGGAGCCGACTATGCAATTTTCCGAACAATGGCTGAGAAGCTGGGTCAATCCGGCGCTGAACACCGAACAATT
GTCCGACCTGCTGACCATGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNNVDAILQAGQAAVAAAADLIELEQVKARFLGKSGELTELLKQLGKLAPEERKAAGATINQAKQAFEAAHNDKRDQLNA
AKLEAQLAAEALDVTLPGRGSVGGGLHPVALTLERIAGLFRTMGFEVADGPEIESDFYNFQALNIPENHPARAMQDTFYV
ENCDVLRTHTSPIQARFMESHQPPIKIVAPGRVYRVDSDATHSPMFHQMEGLWVEEGVSFADLKAVVTDFLRRFFERDDL
QVRFRPSFFPFTETSAEIDVLDEQGKWLEVGGCGMVHPKVLSIANIDPEKYTGFAFGIGLDRFAMLRYGVNDLRLFFEND
LNFLKQFN

Sequences:

>Translated_328_residues
MNNVDAILQAGQAAVAAAADLIELEQVKARFLGKSGELTELLKQLGKLAPEERKAAGATINQAKQAFEAAHNDKRDQLNA
AKLEAQLAAEALDVTLPGRGSVGGGLHPVALTLERIAGLFRTMGFEVADGPEIESDFYNFQALNIPENHPARAMQDTFYV
ENCDVLRTHTSPIQARFMESHQPPIKIVAPGRVYRVDSDATHSPMFHQMEGLWVEEGVSFADLKAVVTDFLRRFFERDDL
QVRFRPSFFPFTETSAEIDVLDEQGKWLEVGGCGMVHPKVLSIANIDPEKYTGFAFGIGLDRFAMLRYGVNDLRLFFEND
LNFLKQFN
>Mature_328_residues
MNNVDAILQAGQAAVAAAADLIELEQVKARFLGKSGELTELLKQLGKLAPEERKAAGATINQAKQAFEAAHNDKRDQLNA
AKLEAQLAAEALDVTLPGRGSVGGGLHPVALTLERIAGLFRTMGFEVADGPEIESDFYNFQALNIPENHPARAMQDTFYV
ENCDVLRTHTSPIQARFMESHQPPIKIVAPGRVYRVDSDATHSPMFHQMEGLWVEEGVSFADLKAVVTDFLRRFFERDDL
QVRFRPSFFPFTETSAEIDVLDEQGKWLEVGGCGMVHPKVLSIANIDPEKYTGFAFGIGLDRFAMLRYGVNDLRLFFEND
LNFLKQFN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=279, Percent_Identity=31.5412186379928, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=31.5985130111524, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=57.4923547400612, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=238, Percent_Identity=34.0336134453782, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=259, Percent_Identity=32.046332046332, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=44.4444444444444, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18858079, Length=286, Percent_Identity=29.3706293706294, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17137424, Length=281, Percent_Identity=27.7580071174377, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CHRVO (Q7NYC2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901022.1
- ProteinModelPortal:   Q7NYC2
- SMR:   Q7NYC2
- GeneID:   2548805
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1352
- NMPDR:   fig|243365.1.peg.1352
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q7NYC2
- ProtClustDB:   PRK00488
- BioCyc:   CVIO243365:CV_1352-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 36461; Mature: 36461

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVDAILQAGQAAVAAAADLIELEQVKARFLGKSGELTELLKQLGKLAPEERKAAGATI
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCHH
NQAKQAFEAAHNDKRDQLNAAKLEAQLAAEALDVTLPGRGSVGGGLHPVALTLERIAGLF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHH
RTMGFEVADGPEIESDFYNFQALNIPENHPARAMQDTFYVENCDVLRTHTSPIQARFMES
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHEEECCCCEEECCCCHHHHHHHHC
HQPPIKIVAPGRVYRVDSDATHSPMFHQMEGLWVEEGVSFADLKAVVTDFLRRFFERDDL
CCCCEEEECCCEEEEECCCCCCCCHHHHHCCEEHHHCCCHHHHHHHHHHHHHHHHCCCCC
QVRFRPSFFPFTETSAEIDVLDEQGKWLEVGGCGMVHPKVLSIANIDPEKYTGFAFGIGL
EEEECCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCHHEEEECCCHHHHCCEEEECCH
DRFAMLRYGVNDLRLFFENDLNFLKQFN
HHHHHHHHCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNVDAILQAGQAAVAAAADLIELEQVKARFLGKSGELTELLKQLGKLAPEERKAAGATI
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCHH
NQAKQAFEAAHNDKRDQLNAAKLEAQLAAEALDVTLPGRGSVGGGLHPVALTLERIAGLF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHH
RTMGFEVADGPEIESDFYNFQALNIPENHPARAMQDTFYVENCDVLRTHTSPIQARFMES
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHEEECCCCEEECCCCHHHHHHHHC
HQPPIKIVAPGRVYRVDSDATHSPMFHQMEGLWVEEGVSFADLKAVVTDFLRRFFERDDL
CCCCEEEECCCEEEEECCCCCCCCHHHHHCCEEHHHCCCHHHHHHHHHHHHHHHHCCCCC
QVRFRPSFFPFTETSAEIDVLDEQGKWLEVGGCGMVHPKVLSIANIDPEKYTGFAFGIGL
EEEECCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCHHEEEECCCHHHHCCEEEECCH
DRFAMLRYGVNDLRLFFENDLNFLKQFN
HHHHHHHHCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782