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The map label for this gene is pheT

Identifier: 34496808

GI number: 34496808

Start: 1424675

End: 1427032

Strand: Direct

Name: pheT

Synonym: CV_1353

Alternate gene names: 34496808

Gene position: 1424675-1427032 (Clockwise)

Preceding gene: 34496807

Following gene: 34496809

Centisome position: 29.99

GC content: 67.47

Gene sequence:

>2358_bases
ATGCAATTTTCCGAACAATGGCTGAGAAGCTGGGTCAATCCGGCGCTGAACACCGAACAATTGTCCGACCTGCTGACCAT
GGCGGGACTGGAAGTGGAAGAAACCGTCGCCGCCGCGCCGGCCTTCAGCGGCGTGGTGATCGCCGAGGTGAAGGAGTGCG
TCAAGCACGAGAACGCCGACCGCCTGCGCGTGACCAAGGTTGACGCCGGTACCGGCGAGCTGATCCAGATCGTCTGCGGC
GCGCCCAATGTCGCCGTCGGCGTGCGCGTGCCGTGCGCGCTGCCGGGCGCGGTGCTGCCGGGCGAATTCAAGATCAAGCC
GACCAAGATGCGCGGCGTGGAATCCAACGGCATGCTGTGCTCCGGCAAGGAGCTGGGCGTGCCGGAGGACGTGGACGGCC
TGATGCTGCTGCCGGCCGACGCGCCGGTGGGCCAGAGCATCCGCGACTATCTGGGCCTGGACGACCAGCTGTTCACGCTG
AAGATCACGCCGAACCGCGCCGACAGCCTGTCGATCCGCGGCATCGCCCGCGAAGTGGCGGCCCTGACCGGCGCCGAGTT
GAAGCCGGTGGCGATCGAGCCGGTGGCGCCGTCCATCGACGACGTCCGTCCGGTGAAGATCGAGTCGCGCCAGGCTTGCG
GCCGCTACCTGGGACGCGTGGTCAAGGGCGTCAATGCCGCCGCGCCGACGCCTGCCTGGATGCGCCGGCGCCTGGAGCGC
TCCGGCCTGCGTTCGATCTCCGCCATCGTCGACATCACCAACTACATTCTGCTGGAGCAGGGCCAGCCGATGCATGCTTT
CGATCTGGCCAAGATCGACGGCGGCATCACCGTCCGCCAGGCCCGCGCCGGCGAGAGCCTGCTGTGCCTGAACGAAAAAA
CCGTCGAGCTGCAGCCCAAGCACCTGGTGATCGCCGACGACGCCAAGCCGCTGGCCATGGGCGGCATCATGGGCGGCGAG
CACAGCGGCGTCACTGTCGCGAGCCACGATATTTTCCTGGAGTCGGCGTTCTTCGCGCCTGAGGCCATCGCCGGCAAGGC
GCGCGAGCTCGGCTTCGGCTCGGACTCGTCCTACCGTTACGAGCGCGGCGTCGATTTCGCGCTGCAGCGCGACGCGATCG
AGCGCGCCACCCGGCTGGTGCTGGAAATCTGCGGCGGCCAGGCCGGCCCGGTGGTGGAGGAAGTGGCAGAGCTGCCGAAG
CGCCAGGCTGTCCAGCTGCGCGCCGCGCGCGTGGCCAAGGTGCTGGGCGTGGCGATGTCCGCCGAGCGGATCGGCGACAT
CCTGTCCCGCCTGAGCCTATCCTATACGCTGGCCGATGAGGTCTTCACCGTGCAGGCGCCGTCGTTCCGCTTCGACATCG
AGATCGAGGAAGACCTGATCGAGGAAGTGGCGCGTGTCTACGGTTATAACAACATCCCGGCCGACGCGCCGCGCTCCGGC
ATGCGCATGCTGGCGCAGCCGGAGGATAAGCGCCCGGTGGATCAGCTGCGCCACCTGCTGGCCGGCCGCGACTACCAGGA
AGTCGTCAGCTATGCCTTCGTCGACGAACGCTGGGAGCGCGATTTCGCCGGCAACGACAACCCGATCCGTCTGCAGAACC
CGATCGCCAGCCAGATGAGCGTGATGCGCTCGACGCTGATCGGCGGCCTGGTCGACGTGTTGGCCGGCAACATCAACCGC
AAGCAGCCGCGCGTGCGGCTGTTCGAAGTGGCGCGCGTGTTCCTGCGCGGCGGGAATGGTTTCGACCAGCCTGAAAAGGT
GGCCGGCCTGGTATGGGGCCCGCGTCTGGCGGAGCAATGGGCGGCCAAGTCCGAGCGCGTCGACTTCTTCGACGTGAAGG
CCGATGTCGAGGCGCTGCTGCATCCGATCAAGGCCGAGTACCGCAAGGCCGCGCATCCCGCCTTCCACCCCGGCCGCTGC
GCCGAGGTGCGGGTGGACGGCAAGGCGGTCGGCGTGATCGGCGAGCTGCACCCGCAGTGGGTGCAGGCCTATGGCCTGAT
CAATGCGCCGGTGTTGTTCGAGCTGGATCTGGCGGCCGCCATCGCGCGCGGCCGGATCAAGGCGCAGCCGGTCAGCAAGT
TCCAACCGGTGCGCCGCGACCTGGCGTTGCTGGTGGACGAAGCGGTGACTGTGGCGCAACTGTTGTCCGCCTTCGCGGCC
AAACGCGCGGAAATCGTCGCCGATATCGCGTTGTTCGACGTTTATCGCGGCAAGGGCGTGGCCGAAGGCAAGAAGAGTCT
GGCCTTTAGCGTGCTGATGCAGGACAATAGCCGGACCTTGACCGACGAGGACGTGGAGCCGGCGATGCAGGCCTTGCTCG
AAGCGGCGTCGGAACTGGGCGCGCAGCTGCGCGTGTGA

Upstream 100 bases:

>100_bases
TTTCGCCATGCTGCGCTACGGCGTCAACGACCTGCGCCTGTTCTTCGAGAACGATCTCAACTTCCTGAAGCAATTCAACT
GATCGCGCACGGAGCCGACT

Downstream 100 bases:

>100_bases
TCGAGATGTGGGCGGGCGGATTCGAACGGGTCCGCCCGTCGATTGTGGTTCAGAAGTTTGAGGGGAATGTCATGACTTTG
ACGAAGGCTGAACTGGCCGA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 785; Mature: 785

Protein sequence:

>785_residues
MQFSEQWLRSWVNPALNTEQLSDLLTMAGLEVEETVAAAPAFSGVVIAEVKECVKHENADRLRVTKVDAGTGELIQIVCG
APNVAVGVRVPCALPGAVLPGEFKIKPTKMRGVESNGMLCSGKELGVPEDVDGLMLLPADAPVGQSIRDYLGLDDQLFTL
KITPNRADSLSIRGIAREVAALTGAELKPVAIEPVAPSIDDVRPVKIESRQACGRYLGRVVKGVNAAAPTPAWMRRRLER
SGLRSISAIVDITNYILLEQGQPMHAFDLAKIDGGITVRQARAGESLLCLNEKTVELQPKHLVIADDAKPLAMGGIMGGE
HSGVTVASHDIFLESAFFAPEAIAGKARELGFGSDSSYRYERGVDFALQRDAIERATRLVLEICGGQAGPVVEEVAELPK
RQAVQLRAARVAKVLGVAMSAERIGDILSRLSLSYTLADEVFTVQAPSFRFDIEIEEDLIEEVARVYGYNNIPADAPRSG
MRMLAQPEDKRPVDQLRHLLAGRDYQEVVSYAFVDERWERDFAGNDNPIRLQNPIASQMSVMRSTLIGGLVDVLAGNINR
KQPRVRLFEVARVFLRGGNGFDQPEKVAGLVWGPRLAEQWAAKSERVDFFDVKADVEALLHPIKAEYRKAAHPAFHPGRC
AEVRVDGKAVGVIGELHPQWVQAYGLINAPVLFELDLAAAIARGRIKAQPVSKFQPVRRDLALLVDEAVTVAQLLSAFAA
KRAEIVADIALFDVYRGKGVAEGKKSLAFSVLMQDNSRTLTDEDVEPAMQALLEAASELGAQLRV

Sequences:

>Translated_785_residues
MQFSEQWLRSWVNPALNTEQLSDLLTMAGLEVEETVAAAPAFSGVVIAEVKECVKHENADRLRVTKVDAGTGELIQIVCG
APNVAVGVRVPCALPGAVLPGEFKIKPTKMRGVESNGMLCSGKELGVPEDVDGLMLLPADAPVGQSIRDYLGLDDQLFTL
KITPNRADSLSIRGIAREVAALTGAELKPVAIEPVAPSIDDVRPVKIESRQACGRYLGRVVKGVNAAAPTPAWMRRRLER
SGLRSISAIVDITNYILLEQGQPMHAFDLAKIDGGITVRQARAGESLLCLNEKTVELQPKHLVIADDAKPLAMGGIMGGE
HSGVTVASHDIFLESAFFAPEAIAGKARELGFGSDSSYRYERGVDFALQRDAIERATRLVLEICGGQAGPVVEEVAELPK
RQAVQLRAARVAKVLGVAMSAERIGDILSRLSLSYTLADEVFTVQAPSFRFDIEIEEDLIEEVARVYGYNNIPADAPRSG
MRMLAQPEDKRPVDQLRHLLAGRDYQEVVSYAFVDERWERDFAGNDNPIRLQNPIASQMSVMRSTLIGGLVDVLAGNINR
KQPRVRLFEVARVFLRGGNGFDQPEKVAGLVWGPRLAEQWAAKSERVDFFDVKADVEALLHPIKAEYRKAAHPAFHPGRC
AEVRVDGKAVGVIGELHPQWVQAYGLINAPVLFELDLAAAIARGRIKAQPVSKFQPVRRDLALLVDEAVTVAQLLSAFAA
KRAEIVADIALFDVYRGKGVAEGKKSLAFSVLMQDNSRTLTDEDVEPAMQALLEAASELGAQLRV
>Mature_785_residues
MQFSEQWLRSWVNPALNTEQLSDLLTMAGLEVEETVAAAPAFSGVVIAEVKECVKHENADRLRVTKVDAGTGELIQIVCG
APNVAVGVRVPCALPGAVLPGEFKIKPTKMRGVESNGMLCSGKELGVPEDVDGLMLLPADAPVGQSIRDYLGLDDQLFTL
KITPNRADSLSIRGIAREVAALTGAELKPVAIEPVAPSIDDVRPVKIESRQACGRYLGRVVKGVNAAAPTPAWMRRRLER
SGLRSISAIVDITNYILLEQGQPMHAFDLAKIDGGITVRQARAGESLLCLNEKTVELQPKHLVIADDAKPLAMGGIMGGE
HSGVTVASHDIFLESAFFAPEAIAGKARELGFGSDSSYRYERGVDFALQRDAIERATRLVLEICGGQAGPVVEEVAELPK
RQAVQLRAARVAKVLGVAMSAERIGDILSRLSLSYTLADEVFTVQAPSFRFDIEIEEDLIEEVARVYGYNNIPADAPRSG
MRMLAQPEDKRPVDQLRHLLAGRDYQEVVSYAFVDERWERDFAGNDNPIRLQNPIASQMSVMRSTLIGGLVDVLAGNINR
KQPRVRLFEVARVFLRGGNGFDQPEKVAGLVWGPRLAEQWAAKSERVDFFDVKADVEALLHPIKAEYRKAAHPAFHPGRC
AEVRVDGKAVGVIGELHPQWVQAYGLINAPVLFELDLAAAIARGRIKAQPVSKFQPVRRDLALLVDEAVTVAQLLSAFAA
KRAEIVADIALFDVYRGKGVAEGKKSLAFSVLMQDNSRTLTDEDVEPAMQALLEAASELGAQLRV

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=395, Percent_Identity=26.0759493670886, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1788006, Length=794, Percent_Identity=47.3551637279597, Blast_Score=706, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17533403, Length=549, Percent_Identity=21.8579234972678, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355211, Length=395, Percent_Identity=26.5822784810127, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_CHRVO (Q7NYC1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901023.1
- ProteinModelPortal:   Q7NYC1
- GeneID:   2551268
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1353
- NMPDR:   fig|243365.1.peg.1353
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- PhylomeDB:   Q7NYC1
- ProtClustDB:   PRK00629
- BioCyc:   CVIO243365:CV_1353-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 85348; Mature: 85348

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSEQWLRSWVNPALNTEQLSDLLTMAGLEVEETVAAAPAFSGVVIAEVKECVKHENAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
RLRVTKVDAGTGELIQIVCGAPNVAVGVRVPCALPGAVLPGEFKIKPTKMRGVESNGMLC
CEEEEEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEECCHHCCCCCCCCCEE
SGKELGVPEDVDGLMLLPADAPVGQSIRDYLGLDDQLFTLKITPNRADSLSIRGIAREVA
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ALTGAELKPVAIEPVAPSIDDVRPVKIESRQACGRYLGRVVKGVNAAAPTPAWMRRRLER
HHCCCCCCCEEECCCCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SGLRSISAIVDITNYILLEQGQPMHAFDLAKIDGGITVRQARAGESLLCLNEKTVELQPK
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCCEEEEECCCCCEEEEECCCCEEECCC
HLVIADDAKPLAMGGIMGGEHSGVTVASHDIFLESAFFAPEAIAGKARELGFGSDSSYRY
EEEEECCCCCEEECCCCCCCCCCEEEEECHHHHHHHHCCCHHHCCCHHHCCCCCCCCCCH
ERGVDFALQRDAIERATRLVLEICGGQAGPVVEEVAELPKRQAVQLRAARVAKVLGVAMS
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AERIGDILSRLSLSYTLADEVFTVQAPSFRFDIEIEEDLIEEVARVYGYNNIPADAPRSG
HHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHH
MRMLAQPEDKRPVDQLRHLLAGRDYQEVVSYAFVDERWERDFAGNDNPIRLQNPIASQMS
HHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHH
VMRSTLIGGLVDVLAGNINRKQPRVRLFEVARVFLRGGNGFDQPEKVAGLVWGPRLAEQW
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHH
AAKSERVDFFDVKADVEALLHPIKAEYRKAAHPAFHPGRCAEVRVDGKAVGVIGELHPQW
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEEHHCCHHH
VQAYGLINAPVLFELDLAAAIARGRIKAQPVSKFQPVRRDLALLVDEAVTVAQLLSAFAA
HHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRAEIVADIALFDVYRGKGVAEGKKSLAFSVLMQDNSRTLTDEDVEPAMQALLEAASELG
HHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
AQLRV
CCCCC
>Mature Secondary Structure
MQFSEQWLRSWVNPALNTEQLSDLLTMAGLEVEETVAAAPAFSGVVIAEVKECVKHENAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
RLRVTKVDAGTGELIQIVCGAPNVAVGVRVPCALPGAVLPGEFKIKPTKMRGVESNGMLC
CEEEEEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEECCHHCCCCCCCCCEE
SGKELGVPEDVDGLMLLPADAPVGQSIRDYLGLDDQLFTLKITPNRADSLSIRGIAREVA
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ALTGAELKPVAIEPVAPSIDDVRPVKIESRQACGRYLGRVVKGVNAAAPTPAWMRRRLER
HHCCCCCCCEEECCCCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SGLRSISAIVDITNYILLEQGQPMHAFDLAKIDGGITVRQARAGESLLCLNEKTVELQPK
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCCEEEEECCCCCEEEEECCCCEEECCC
HLVIADDAKPLAMGGIMGGEHSGVTVASHDIFLESAFFAPEAIAGKARELGFGSDSSYRY
EEEEECCCCCEEECCCCCCCCCCEEEEECHHHHHHHHCCCHHHCCCHHHCCCCCCCCCCH
ERGVDFALQRDAIERATRLVLEICGGQAGPVVEEVAELPKRQAVQLRAARVAKVLGVAMS
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AERIGDILSRLSLSYTLADEVFTVQAPSFRFDIEIEEDLIEEVARVYGYNNIPADAPRSG
HHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCHH
MRMLAQPEDKRPVDQLRHLLAGRDYQEVVSYAFVDERWERDFAGNDNPIRLQNPIASQMS
HHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHH
VMRSTLIGGLVDVLAGNINRKQPRVRLFEVARVFLRGGNGFDQPEKVAGLVWGPRLAEQW
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHH
AAKSERVDFFDVKADVEALLHPIKAEYRKAAHPAFHPGRCAEVRVDGKAVGVIGELHPQW
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEEHHCCHHH
VQAYGLINAPVLFELDLAAAIARGRIKAQPVSKFQPVRRDLALLVDEAVTVAQLLSAFAA
HHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRAEIVADIALFDVYRGKGVAEGKKSLAFSVLMQDNSRTLTDEDVEPAMQALLEAASELG
HHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
AQLRV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782