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The map label for this gene is tgt

Identifier: 34496802

GI number: 34496802

Start: 1418833

End: 1419948

Strand: Reverse

Name: tgt

Synonym: CV_1347

Alternate gene names: 34496802

Gene position: 1419948-1418833 (Counterclockwise)

Preceding gene: 34496814

Following gene: 34496801

Centisome position: 29.89

GC content: 64.16

Gene sequence:

>1116_bases
ATGTTGAAGTTTACCGTACACAAGACCTCCGGCGGCGCGCGTCGCGGCACGCTGGAGCTGAACCACGGCACCGTGGAGAC
CCCGGTCTTCCAGCCGGTGGGCACTTACGGCTCGGTCAAGGCGATGAGCCCGGTCGAGCTGAACGACATCGGCGCCCAGA
TCATCCTGGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCTGGAGATCGTCGAGCAGTTCGGCGGCCTGCACGAATTC
ATCGGCTGGGACAAGCCCATCCTCACCGACTCCGGCGGCTTCCAGGTGTTCAGCCTGTCCGACATGCGCAAGCTGACCGA
GGAAGGCTGCACCTTCCAGAGCCCGATCAACGGCGACAAGCTGTTCCTCTCGCCTGAGATCTCGATGAAGATCCAGACGG
TGCTCAACTCGGACATCGTGATGCAGCTGGACGAGTGCACGCCGGGCCAGGTGGACCACGCCACCGCGCAGAAGTCGCTG
CAGATGAGCCTGCGCTGGGCCGAGCGCTCGCGCCGCGCCTTCGACGACCTGAAAAACCCCAACGCGCTGTTCGGCATCGT
GCAAGGCAACCTTTATACCGATTTGCGCCAGGAGTCGCTGGAAGGCCTGATGCAGGTCGGCTTCGACGGCATCGCCATCG
GCGGCCTGTCGGTGGGCGAGCCCAAGCCGGAAATGTACCGGATGCTGACCGAGCTGAAGGACATGCTGCCGGCCGACAAG
CCGCACTACCTGATGGGCGTCGGCACGCCGGAAGACCTGGTGCACGGCGTGGCCAACGGCGTGGACATGTTCGACTGCGT
GATGCCGACCCGCAACGCGCGCAACGGCTGGATCTTCACCCAGTGGGGCGACGTCAAGATCAAGAACGCGCGCTACAAGG
ACGACAAGAAGCCGCTGGACGAGGAATGCGCCTGCTACGCCTGCCGCAACTTCAGCCGCGCCTACCTGCACCACCTGCAC
CGCGTCGGCGAAATCCTGGGCGCGCGGCTGAACACCATACACAATCTGTTCTACTACCAGGAACTGATGCGCGAGATGCG
CAAGGCGATCGAGGAAGACCGCTTCGAGGACTTCCGCCTGGAATTCGCCGCCAAGCGCGCCCGCAGCGTCAACTGA

Upstream 100 bases:

>100_bases
CTTGGCAAGCACTTTTTTGCGATTCCGGCGCAGCCCGCGCGCTTGCTGTATACTAGCGGACTGATTTTGCTAAACAAACC
CTGAAGCGATACTTCGAATA

Downstream 100 bases:

>100_bases
GGCGCGCCGGGCGGGGAACCGCGGCCCGGCGCCGACGGTCCCACGCCCTTGCCGCGCTCCGTGGTTGGAAAAGCCATGGA
GCATGGCTACAATGCCCTGT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLKFTVHKTSGGARRGTLELNHGTVETPVFQPVGTYGSVKAMSPVELNDIGAQIILGNTFHLWLRPGLEIVEQFGGLHEF
IGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFQSPINGDKLFLSPEISMKIQTVLNSDIVMQLDECTPGQVDHATAQKSL
QMSLRWAERSRRAFDDLKNPNALFGIVQGNLYTDLRQESLEGLMQVGFDGIAIGGLSVGEPKPEMYRMLTELKDMLPADK
PHYLMGVGTPEDLVHGVANGVDMFDCVMPTRNARNGWIFTQWGDVKIKNARYKDDKKPLDEECACYACRNFSRAYLHHLH
RVGEILGARLNTIHNLFYYQELMREMRKAIEEDRFEDFRLEFAAKRARSVN

Sequences:

>Translated_371_residues
MLKFTVHKTSGGARRGTLELNHGTVETPVFQPVGTYGSVKAMSPVELNDIGAQIILGNTFHLWLRPGLEIVEQFGGLHEF
IGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFQSPINGDKLFLSPEISMKIQTVLNSDIVMQLDECTPGQVDHATAQKSL
QMSLRWAERSRRAFDDLKNPNALFGIVQGNLYTDLRQESLEGLMQVGFDGIAIGGLSVGEPKPEMYRMLTELKDMLPADK
PHYLMGVGTPEDLVHGVANGVDMFDCVMPTRNARNGWIFTQWGDVKIKNARYKDDKKPLDEECACYACRNFSRAYLHHLH
RVGEILGARLNTIHNLFYYQELMREMRKAIEEDRFEDFRLEFAAKRARSVN
>Mature_371_residues
MLKFTVHKTSGGARRGTLELNHGTVETPVFQPVGTYGSVKAMSPVELNDIGAQIILGNTFHLWLRPGLEIVEQFGGLHEF
IGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFQSPINGDKLFLSPEISMKIQTVLNSDIVMQLDECTPGQVDHATAQKSL
QMSLRWAERSRRAFDDLKNPNALFGIVQGNLYTDLRQESLEGLMQVGFDGIAIGGLSVGEPKPEMYRMLTELKDMLPADK
PHYLMGVGTPEDLVHGVANGVDMFDCVMPTRNARNGWIFTQWGDVKIKNARYKDDKKPLDEECACYACRNFSRAYLHHLH
RVGEILGARLNTIHNLFYYQELMREMRKAIEEDRFEDFRLEFAAKRARSVN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=43.3701657458564, Blast_Score=305, Evalue=3e-83,
Organism=Escherichia coli, GI1786607, Length=369, Percent_Identity=63.4146341463415, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=41.8732782369146, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=137, Percent_Identity=29.9270072992701, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=42.7792915531335, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI21355311, Length=156, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CHRVO (Q7NYC7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901017.1
- HSSP:   P28720
- ProteinModelPortal:   Q7NYC7
- SMR:   Q7NYC7
- GeneID:   2548605
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1347
- NMPDR:   fig|243365.1.peg.1347
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q7NYC7
- ProtClustDB:   PRK00112
- BioCyc:   CVIO243365:CV_1347-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41938; Mature: 41938

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 90-90 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFTVHKTSGGARRGTLELNHGTVETPVFQPVGTYGSVKAMSPVELNDIGAQIILGNTF
CEEEEEEECCCCCCCCEEEECCCEECCCCCCCCCCCCCCCCCCCCCHHCCCCEEEECCEE
HLWLRPGLEIVEQFGGLHEFIGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFQSPINGDK
EEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCCCE
LFLSPEISMKIQTVLNSDIVMQLDECTPGQVDHATAQKSLQMSLRWAERSRRAFDDLKNP
EEECCCCCEEHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NALFGIVQGNLYTDLRQESLEGLMQVGFDGIAIGGLSVGEPKPEMYRMLTELKDMLPADK
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
PHYLMGVGTPEDLVHGVANGVDMFDCVMPTRNARNGWIFTQWGDVKIKNARYKDDKKPLD
CCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCH
EECACYACRNFSRAYLHHLHRVGEILGARLNTIHNLFYYQELMREMRKAIEEDRFEDFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EFAAKRARSVN
HHHHHHHCCCC
>Mature Secondary Structure
MLKFTVHKTSGGARRGTLELNHGTVETPVFQPVGTYGSVKAMSPVELNDIGAQIILGNTF
CEEEEEEECCCCCCCCEEEECCCEECCCCCCCCCCCCCCCCCCCCCHHCCCCEEEECCEE
HLWLRPGLEIVEQFGGLHEFIGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFQSPINGDK
EEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCCCE
LFLSPEISMKIQTVLNSDIVMQLDECTPGQVDHATAQKSLQMSLRWAERSRRAFDDLKNP
EEECCCCCEEHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NALFGIVQGNLYTDLRQESLEGLMQVGFDGIAIGGLSVGEPKPEMYRMLTELKDMLPADK
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
PHYLMGVGTPEDLVHGVANGVDMFDCVMPTRNARNGWIFTQWGDVKIKNARYKDDKKPLD
CCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCH
EECACYACRNFSRAYLHHLHRVGEILGARLNTIHNLFYYQELMREMRKAIEEDRFEDFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EFAAKRARSVN
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782