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The map label for this gene is glyA

Identifier: 34496741

GI number: 34496741

Start: 1353415

End: 1354662

Strand: Direct

Name: glyA

Synonym: CV_1286

Alternate gene names: 34496741

Gene position: 1353415-1354662 (Clockwise)

Preceding gene: 34496740

Following gene: 34496742

Centisome position: 28.49

GC content: 64.66

Gene sequence:

>1248_bases
ATGTTCGCTGCTGACCAGACGATCGCCAAATTCGACCCAGAACTCGCCGCCGCCATCGCGGCTGAATGCCAACGCCAGGA
AGATCACATCGAGCTGATCGCCTCGGAAAACTACACCAGCCCGGCCGTCATGGAAGCCCAAGGCTCCCAGCTGACCAACA
AGTACGCCGAAGGCTATCCGGGCAAGCGCTTCTACGGCGGTTGCGAGCATGTGGATGTCGTCGAGCAACTGGCCATCGAC
CGCGTCAAGCAGCTGTTCGGCGCCGAATACGCCAACGTGCAGCCGCACTCCGGCTCCCAGGCCAACCAGGCGGTGTACTT
CTCCATCCTGAAGCCGGGCGACACCGTGATGGGCATGAACCTGGGCCACGGCGGCCACCTGACCCACGGCTCCCCGGCTA
ACCTGTCCGGCAAGATGTTCAACATCGTCGCCTACGGCCTGAACGACAAGGAAGAAATCGACTACGACGACATGGAGCGC
GTGGCGATGGAAACCAAGCCGAAGCTGATCATCGGCGGCGCGTCCGCCTACGCGCTGCGCTTCGACTTCGAGCGCATGGG
CCAGATCGCCAAGAAAGTCGGCGCCTACTTCATGGTCGACATGGCCCACTACGCCGGCCTGGTGGCCGCGGGCCTGTACC
CGAACCCGGTGCCGCACGCCGACTTCGTGACCTCCACCACCCACAAGACCCTGCGCGGTCCGCGCGGCGGCATCATCCTG
GCCAAGGCAGAGTTCGAGAAGAGCATCAACAGCAACGTGTTCCCGACGCTGCAGGGCGGTCCGCTGGAGCATGTGATCGC
CGCCAAGGCCGTCGCCTTCAAGGAAGCGCTGCAGCCGGCATTCAAGGAATACCAGCAGCAGGTGCTGAAGAACGCCGCCA
TCATGGCCAAGACCCTGGCCGAGCGCGGTTTGCGCATCGTGTCCGGCCGCACCGAGAGCCACGTGTTCCTGGTGGACCTG
CGCGCCAAGGGCCTGACCGGCAAGCAAGCCGACGCGCTGCTGGGCCGCGCCCACATCACCGTCAACAAGAACGCGATCCC
GAACGATCCGGAAACCCCGTTCGTCACCTCCGGCATCCGCATCGGCTCCCCGGCCATCACCACCCGCGGCTTCAAGGAAG
CCGAGGCCATCGAAGTGGCCAATATGGTCGCCGACGTGCTGGACAACCCGAACGACGACGCGCTGATCGCCCGCATCGCC
GAGAAGGCCACCGCGCTGTGCCACCGCTTCCCGGTGTACGCCAAGTAA

Upstream 100 bases:

>100_bases
CGCGGCAAGGCCTCGGCGAGCTTGCTTCACGTTAAGGTTTCGTTCCGGGCATTTGGTGTAGAATGCCCGGATCGTTAACC
CTTTGATGGATGAGTCAAAG

Downstream 100 bases:

>100_bases
TCGACCGGTTCGCCGGATGCAGAACAGCCGCCCCAGGGGCGGCTTTTTCTTTGCGCCAGGCCAGGCTTCGTCCATGGCCG
GCGGTGCGGGCGGGCGGCAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MFAADQTIAKFDPELAAAIAAECQRQEDHIELIASENYTSPAVMEAQGSQLTNKYAEGYPGKRFYGGCEHVDVVEQLAID
RVKQLFGAEYANVQPHSGSQANQAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGKMFNIVAYGLNDKEEIDYDDMER
VAMETKPKLIIGGASAYALRFDFERMGQIAKKVGAYFMVDMAHYAGLVAAGLYPNPVPHADFVTSTTHKTLRGPRGGIIL
AKAEFEKSINSNVFPTLQGGPLEHVIAAKAVAFKEALQPAFKEYQQQVLKNAAIMAKTLAERGLRIVSGRTESHVFLVDL
RAKGLTGKQADALLGRAHITVNKNAIPNDPETPFVTSGIRIGSPAITTRGFKEAEAIEVANMVADVLDNPNDDALIARIA
EKATALCHRFPVYAK

Sequences:

>Translated_415_residues
MFAADQTIAKFDPELAAAIAAECQRQEDHIELIASENYTSPAVMEAQGSQLTNKYAEGYPGKRFYGGCEHVDVVEQLAID
RVKQLFGAEYANVQPHSGSQANQAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGKMFNIVAYGLNDKEEIDYDDMER
VAMETKPKLIIGGASAYALRFDFERMGQIAKKVGAYFMVDMAHYAGLVAAGLYPNPVPHADFVTSTTHKTLRGPRGGIIL
AKAEFEKSINSNVFPTLQGGPLEHVIAAKAVAFKEALQPAFKEYQQQVLKNAAIMAKTLAERGLRIVSGRTESHVFLVDL
RAKGLTGKQADALLGRAHITVNKNAIPNDPETPFVTSGIRIGSPAITTRGFKEAEAIEVANMVADVLDNPNDDALIARIA
EKATALCHRFPVYAK
>Mature_415_residues
MFAADQTIAKFDPELAAAIAAECQRQEDHIELIASENYTSPAVMEAQGSQLTNKYAEGYPGKRFYGGCEHVDVVEQLAID
RVKQLFGAEYANVQPHSGSQANQAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGKMFNIVAYGLNDKEEIDYDDMER
VAMETKPKLIIGGASAYALRFDFERMGQIAKKVGAYFMVDMAHYAGLVAAGLYPNPVPHADFVTSTTHKTLRGPRGGIIL
AKAEFEKSINSNVFPTLQGGPLEHVIAAKAVAFKEALQPAFKEYQQQVLKNAAIMAKTLAERGLRIVSGRTESHVFLVDL
RAKGLTGKQADALLGRAHITVNKNAIPNDPETPFVTSGIRIGSPAITTRGFKEAEAIEVANMVADVLDNPNDDALIARIA
EKATALCHRFPVYAK

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=407, Percent_Identity=45.7002457002457, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI261862350, Length=407, Percent_Identity=45.7002457002457, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI261862348, Length=407, Percent_Identity=45.7002457002457, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI19923315, Length=407, Percent_Identity=45.7002457002457, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI261862346, Length=406, Percent_Identity=44.8275862068966, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI22547186, Length=403, Percent_Identity=43.424317617866, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=41.4948453608247, Blast_Score=266, Evalue=3e-71,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=67.1462829736211, Blast_Score=563, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=45.5, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=45.5, Blast_Score=333, Evalue=8e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=43.142144638404, Blast_Score=328, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6323087, Length=389, Percent_Identity=45.2442159383033, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=46.25, Blast_Score=347, Evalue=9e-96,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=46.25, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_CHRVO (Q7NYI8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900956.1
- ProteinModelPortal:   Q7NYI8
- SMR:   Q7NYI8
- GeneID:   2551031
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1286
- NMPDR:   fig|243365.1.peg.1286
- HOGENOM:   HBG301263
- OMA:   HRIDYEE
- PhylomeDB:   Q7NYI8
- ProtClustDB:   PRK00011
- BioCyc:   CVIO243365:CV_1286-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44918; Mature: 44918

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAADQTIAKFDPELAAAIAAECQRQEDHIELIASENYTSPAVMEAQGSQLTNKYAEGYP
CCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHCCCC
GKRFYGGCEHVDVVEQLAIDRVKQLFGAEYANVQPHSGSQANQAVYFSILKPGDTVMGMN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LGHGGHLTHGSPANLSGKMFNIVAYGLNDKEEIDYDDMERVAMETKPKLIIGGASAYALR
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEEE
FDFERMGQIAKKVGAYFMVDMAHYAGLVAAGLYPNPVPHADFVTSTTHKTLRGPRGGIIL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHCCCCCCEEE
AKAEFEKSINSNVFPTLQGGPLEHVIAAKAVAFKEALQPAFKEYQQQVLKNAAIMAKTLA
EEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGLRIVSGRTESHVFLVDLRAKGLTGKQADALLGRAHITVNKNAIPNDPETPFVTSGIR
HCCCEEEECCCCCEEEEEEEECCCCCCCHHHHHHEEEEEEECCCCCCCCCCCCEEECCEE
IGSPAITTRGFKEAEAIEVANMVADVLDNPNDDALIARIAEKATALCHRFPVYAK
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFAADQTIAKFDPELAAAIAAECQRQEDHIELIASENYTSPAVMEAQGSQLTNKYAEGYP
CCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHCCCC
GKRFYGGCEHVDVVEQLAIDRVKQLFGAEYANVQPHSGSQANQAVYFSILKPGDTVMGMN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LGHGGHLTHGSPANLSGKMFNIVAYGLNDKEEIDYDDMERVAMETKPKLIIGGASAYALR
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEEE
FDFERMGQIAKKVGAYFMVDMAHYAGLVAAGLYPNPVPHADFVTSTTHKTLRGPRGGIIL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHCCCCCCEEE
AKAEFEKSINSNVFPTLQGGPLEHVIAAKAVAFKEALQPAFKEYQQQVLKNAAIMAKTLA
EEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGLRIVSGRTESHVFLVDLRAKGLTGKQADALLGRAHITVNKNAIPNDPETPFVTSGIR
HCCCEEEECCCCCEEEEEEEECCCCCCCHHHHHHEEEEEEECCCCCCCCCCCCEEECCEE
IGSPAITTRGFKEAEAIEVANMVADVLDNPNDDALIARIAEKATALCHRFPVYAK
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782