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The map label for this gene is nfdA [H]

Identifier: 34496740

GI number: 34496740

Start: 1351430

End: 1353310

Strand: Direct

Name: nfdA [H]

Synonym: CV_1285

Alternate gene names: 34496740

Gene position: 1351430-1353310 (Clockwise)

Preceding gene: 34496739

Following gene: 34496741

Centisome position: 28.44

GC content: 69.32

Gene sequence:

>1881_bases
ATGGCTATAGCTGATGTGATTCTGCGCAATGGCCGCATCGCCACGCTGGACCGGCGGCGGCCGCAGGCGAGCGCCGTCGC
CGTCAAGGACGGCCGCTTCCTCGCGGTGGGGGAGGAGGCGGAGATCATGGCGCTGGCCGGGCCTACCACCCGCGTCGTCG
ATCTCAAGGGCCGCCGCGCGCTGCCCGGCCTGATCGACAACCACACCCATCTGGTGCGCGGCGGGCTCAACTTCAATATG
GAGCTGCGCTGGGACGGCGTGCGCTCGCTGGCCGACGCGATGGACATGCTGCGGCGGCAGGTGGCCGTCACGCCCGCCCC
GCAATGGGTGCGGGTGGTGGGCGGCTTCACCGAGCACCAGTTCGTCGAAAAGCGGCTGCCCACGCTGGACGAGATCAACG
CCGTCGCGCCGGACACGCCGGTCTTCCTGCTGCACCTGTATGACCGCGCCCTGCTCAACGGCGCCGCGTTGCGCGCCGTC
GGCTACGGCCTCGACACGCCGGCGCCGCCGGGCGGGGAGATCGTCCGCGACGGCGGCGGCAACCCTACCGGTCTGCTGTT
GGCCAAGCCCAATGCCGCCATCCTGTATGCGACGCTGGCCAAGGGGCCCAAGCTGCCGTTCGACTACCAGCTCAACTCCA
CCCGCCACTTCATGCGCGAACTGAACCGCCTGGGCGTGACCGGCGTCATCGACGCCGGCGGCGGCTTCCAGAACTACCCG
GACGATTACCAGGTGATCCGGCAACTGGCCGACGCCGGACAGATGACGGTGCGGCTGGCCTACAATCTGTTCACCCAGAA
GCCGAAGGCGGAAAAGGAGGATTTCCTGGCCTGGACCGCCGGCGTCCGCTACAAACAGGGCGACGACTATTTCCGCCACA
ACGGCGCTGGCGAGATGCTGGTGTTTTCCGCCGCCGATTTCGAAGACTTCCGCCAGCCGCGGCCCGAGATGCCGGCGGAA
ATGGAGGGCGAGCTGGAAGAGGTGGTGCGCGTCCTGGCGCAGAACCGCTGGCCGTGGCGGCTGCACGCCACCTACGACGA
AACCATCTCCCGCGCGCTGGACGTGTTCGAGAAGGTGGACCGCGACATCCCCTTGCAAGGGCTGAACTGGTTCTTCGACC
ACGCCGAGACCATCTCCGAGCGCTCGATGGACCGCATCGCCGCGCTGGGCGGCGGCATCGCGGTGCAGCACCGGATGGCC
TACCAGGGCGAGTACTTCGTCGAGCGCTACGGCGCGGCGGCGGCCGAGGCCACGCCGCCGATCGCCCGCATGCTGGAAAA
GGGACTGAAAGTGTCGGCCGGCACCGACGCCACCCGCGTCGCCTCCTACAATCCCTGGGTGTCGCTGGCGTGGATGATCA
CCGGCAAGACCGTAGGCGGCCTGCGGCTCTACCCGCAGCGCAACTGCCTGGACCGCGAAACCGCGCTGCGGATGTGGACC
GAGCACGTGGCCTGGTTCACCGACGAGGAGGGCGGGCGCGGCCGCATCGAGGCCGGCCAGCTGGCCGACCTGGTCGTGCC
GGACAAAGACTTCTTCTCCTGCCCGGAGGATGACATCTCCTTTCTGGTGTCGGAGCTGACCATGGTGGGCGGCCGCATCG
TCTACGCCGCGGGCGGTTTCGCCGCGCTGGACGACAATCCTTTGCCGCCGGCGATGCCGGACTGGTCGCCGGCGCGGACC
TTCAAGGGATACGCCGCCTGGGGCGATCCAGACGGCGCCGGCCGCCATTCGCTGCAGCGCCGGGCCGTGGCCGCTTGCGG
CTGCGCCAATGCCTGCGGCCTGCACGGCCACCAGCATGCGCGGGCCTGGGGGACGGCGTTGCCGGTATCGGACGCCAAGG
GTTTTTTCGGCGCGCTCGGCTGCTCCTGCTGGGCGGTGTGA

Upstream 100 bases:

>100_bases
CTACGCGAAAACCATGTTCGGCGGCCACGAAGGCTGATTGCCGCGCCGCCGCCGGCCGCGCGTCCGCCGGCGGCGGCCGA
TATCCAGGGGAGAAGCCACC

Downstream 100 bases:

>100_bases
GAGGCGCGGCAAGGCCTCGGCGAGCTTGCTTCACGTTAAGGTTTCGTTCCGGGCATTTGGTGTAGAATGCCCGGATCGTT
AACCCTTTGATGGATGAGTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MAIADVILRNGRIATLDRRRPQASAVAVKDGRFLAVGEEAEIMALAGPTTRVVDLKGRRALPGLIDNHTHLVRGGLNFNM
ELRWDGVRSLADAMDMLRRQVAVTPAPQWVRVVGGFTEHQFVEKRLPTLDEINAVAPDTPVFLLHLYDRALLNGAALRAV
GYGLDTPAPPGGEIVRDGGGNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGVIDAGGGFQNYP
DDYQVIRQLADAGQMTVRLAYNLFTQKPKAEKEDFLAWTAGVRYKQGDDYFRHNGAGEMLVFSAADFEDFRQPRPEMPAE
MEGELEEVVRVLAQNRWPWRLHATYDETISRALDVFEKVDRDIPLQGLNWFFDHAETISERSMDRIAALGGGIAVQHRMA
YQGEYFVERYGAAAAEATPPIARMLEKGLKVSAGTDATRVASYNPWVSLAWMITGKTVGGLRLYPQRNCLDRETALRMWT
EHVAWFTDEEGGRGRIEAGQLADLVVPDKDFFSCPEDDISFLVSELTMVGGRIVYAAGGFAALDDNPLPPAMPDWSPART
FKGYAAWGDPDGAGRHSLQRRAVAACGCANACGLHGHQHARAWGTALPVSDAKGFFGALGCSCWAV

Sequences:

>Translated_626_residues
MAIADVILRNGRIATLDRRRPQASAVAVKDGRFLAVGEEAEIMALAGPTTRVVDLKGRRALPGLIDNHTHLVRGGLNFNM
ELRWDGVRSLADAMDMLRRQVAVTPAPQWVRVVGGFTEHQFVEKRLPTLDEINAVAPDTPVFLLHLYDRALLNGAALRAV
GYGLDTPAPPGGEIVRDGGGNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGVIDAGGGFQNYP
DDYQVIRQLADAGQMTVRLAYNLFTQKPKAEKEDFLAWTAGVRYKQGDDYFRHNGAGEMLVFSAADFEDFRQPRPEMPAE
MEGELEEVVRVLAQNRWPWRLHATYDETISRALDVFEKVDRDIPLQGLNWFFDHAETISERSMDRIAALGGGIAVQHRMA
YQGEYFVERYGAAAAEATPPIARMLEKGLKVSAGTDATRVASYNPWVSLAWMITGKTVGGLRLYPQRNCLDRETALRMWT
EHVAWFTDEEGGRGRIEAGQLADLVVPDKDFFSCPEDDISFLVSELTMVGGRIVYAAGGFAALDDNPLPPAMPDWSPART
FKGYAAWGDPDGAGRHSLQRRAVAACGCANACGLHGHQHARAWGTALPVSDAKGFFGALGCSCWAV
>Mature_625_residues
AIADVILRNGRIATLDRRRPQASAVAVKDGRFLAVGEEAEIMALAGPTTRVVDLKGRRALPGLIDNHTHLVRGGLNFNME
LRWDGVRSLADAMDMLRRQVAVTPAPQWVRVVGGFTEHQFVEKRLPTLDEINAVAPDTPVFLLHLYDRALLNGAALRAVG
YGLDTPAPPGGEIVRDGGGNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGVIDAGGGFQNYPD
DYQVIRQLADAGQMTVRLAYNLFTQKPKAEKEDFLAWTAGVRYKQGDDYFRHNGAGEMLVFSAADFEDFRQPRPEMPAEM
EGELEEVVRVLAQNRWPWRLHATYDETISRALDVFEKVDRDIPLQGLNWFFDHAETISERSMDRIAALGGGIAVQHRMAY
QGEYFVERYGAAAAEATPPIARMLEKGLKVSAGTDATRVASYNPWVSLAWMITGKTVGGLRLYPQRNCLDRETALRMWTE
HVAWFTDEEGGRGRIEAGQLADLVVPDKDFFSCPEDDISFLVSELTMVGGRIVYAAGGFAALDDNPLPPAMPDWSPARTF
KGYAAWGDPDGAGRHSLQRRAVAACGCANACGLHGHQHARAWGTALPVSDAKGFFGALGCSCWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68750; Mature: 68619

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIADVILRNGRIATLDRRRPQASAVAVKDGRFLAVGEEAEIMALAGPTTRVVDLKGRRA
CCHHHHHHCCCCEEEECCCCCCCCEEEEECCEEEEECCCCCEEEEECCCEEEEECCCCCC
LPGLIDNHTHLVRGGLNFNMELRWDGVRSLADAMDMLRRQVAVTPAPQWVRVVGGFTEHQ
CCCHHCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
FVEKRLPTLDEINAVAPDTPVFLLHLYDRALLNGAALRAVGYGLDTPAPPGGEIVRDGGG
HHHHHCCCHHHCCCCCCCCCEEHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEECCCC
NPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGVIDAGGGFQNYP
CCCEEEEECCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCC
DDYQVIRQLADAGQMTVRLAYNLFTQKPKAEKEDFLAWTAGVRYKQGDDYFRHNGAGEML
HHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCHHHHEEEECCCEECCCCCHHHCCCCCCEE
VFSAADFEDFRQPRPEMPAEMEGELEEVVRVLAQNRWPWRLHATYDETISRALDVFEKVD
EEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHC
RDIPLQGLNWFFDHAETISERSMDRIAALGGGIAVQHRMAYQGEYFVERYGAAAAEATPP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCCCCCH
IARMLEKGLKVSAGTDATRVASYNPWVSLAWMITGKTVGGLRLYPQRNCLDRETALRMWT
HHHHHHHCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHH
EHVAWFTDEEGGRGRIEAGQLADLVVPDKDFFSCPEDDISFLVSELTMVGGRIVYAAGGF
HHHHEEECCCCCCCCCCCCCEEEEECCCCHHCCCCHHHHHHHHHHHHHHCCEEEEEECCE
AALDDNPLPPAMPDWSPARTFKGYAAWGDPDGAGRHSLQRRAVAACGCANACGLHGHQHA
EECCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
RAWGTALPVSDAKGFFGALGCSCWAV
HHHCCCCCCCCCCCHHHHHCCCEECC
>Mature Secondary Structure 
AIADVILRNGRIATLDRRRPQASAVAVKDGRFLAVGEEAEIMALAGPTTRVVDLKGRRA
CHHHHHHCCCCEEEECCCCCCCCEEEEECCEEEEECCCCCEEEEECCCEEEEECCCCCC
LPGLIDNHTHLVRGGLNFNMELRWDGVRSLADAMDMLRRQVAVTPAPQWVRVVGGFTEHQ
CCCHHCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
FVEKRLPTLDEINAVAPDTPVFLLHLYDRALLNGAALRAVGYGLDTPAPPGGEIVRDGGG
HHHHHCCCHHHCCCCCCCCCEEHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEECCCC
NPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGVIDAGGGFQNYP
CCCEEEEECCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCC
DDYQVIRQLADAGQMTVRLAYNLFTQKPKAEKEDFLAWTAGVRYKQGDDYFRHNGAGEML
HHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCHHHHEEEECCCEECCCCCHHHCCCCCCEE
VFSAADFEDFRQPRPEMPAEMEGELEEVVRVLAQNRWPWRLHATYDETISRALDVFEKVD
EEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHC
RDIPLQGLNWFFDHAETISERSMDRIAALGGGIAVQHRMAYQGEYFVERYGAAAAEATPP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCCCCCH
IARMLEKGLKVSAGTDATRVASYNPWVSLAWMITGKTVGGLRLYPQRNCLDRETALRMWT
HHHHHHHCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHH
EHVAWFTDEEGGRGRIEAGQLADLVVPDKDFFSCPEDDISFLVSELTMVGGRIVYAAGGF
HHHHEEECCCCCCCCCCCCCEEEEECCCCHHCCCCHHHHHHHHHHHHHHCCEEEEEECCE
AALDDNPLPPAMPDWSPARTFKGYAAWGDPDGAGRHSLQRRAVAACGCANACGLHGHQHA
EECCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
RAWGTALPVSDAKGFFGALGCSCWAV
HHHCCCCCCCCCCCHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA