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The map label for this gene is ywbI [H]

Identifier: 34496605

GI number: 34496605

Start: 1209138

End: 1210028

Strand: Reverse

Name: ywbI [H]

Synonym: CV_1150

Alternate gene names: 34496605

Gene position: 1210028-1209138 (Counterclockwise)

Preceding gene: 34496608

Following gene: 34496602

Centisome position: 25.47

GC content: 67.68

Gene sequence:

>891_bases
ATGGACATCCGCGCCTTACGCTATTTCGTCGAACTGGTGAAATGCCAGAGTTTCACCCGCGCCGCCGACGCGCTGTTCGT
CACCCAGCCGACGATCAGCAAGATGGTGAAGCAGCTGGAGGAAGAGCTGGACATGCCGCTGATCCTGCGCGAGGGCCGCA
GCTTCCGCCTGACCGACGCCGGCCGCGTCACCTACGAGCGCGGCCTGGACGTGCTGTCGGCGATGAACCAGCTCAAGCAC
GAACTGGCCGACCTGGCCTCGCTCAGCCACGGCGAGCTGGTGGTGGGCCTGCCGCCGATGGTGGGGGTCGCCTTCTTCGC
GCCGGTGGTCAGCCGCTACCGCCGCCGCTATCCGCAGATCGAGCTGAAGATGGTGGAGGACGGCGCGCTGGCGATAGAGA
ACCGCATCAAGAGCGGCGAATTGGAGATAGGCGTGGCGGTGCTGCCGGTGGACAGCCAGCTGTTCGAGCACTACTCGGTG
GTGCGCGATCCGCTGTGCCTGGTGGCGCCCGCCGGCTCGCGCTGGCGCGACAAATCGCTGGTGCAGCTGGCGGACATCGC
CGACCAGCCGCTGGTGCTGTACCCGGACGACTTCACGCTCAGCCGCCGCGTCGGCGACGCCTTCCGCGAACTGGGCAAGA
CGCCCAACATCGTCGGACGCAGCGCGCACTGGGACTTCATCGTCGAACTGGTGGCCGCCAACCTGGGCGTCACCCTGCTG
CCGCGCAGCATCGTCGAGCGGCTGGACCGCCAGCTGTTCGACGTGGTGCCGCTATACGACCACAAGCTGTACTGGCATCT
GGCGTTGATCTGGCAGCGCGGCGGCTATCTGTCCCACGCCGCCCGCGCCTGGCTGGCGCTGACGCGGGAGACGCTGGGCG
GGCAGGATTGA

Upstream 100 bases:

>100_bases
TTGGCTTCCATCAACTTAGGAGTTGAGCAAAGTATAGCCAGCCGGCATTCATTCTAAAAATGAATTAAAATTATCAAGAC
GATTCCATACAGGAATGAAA

Downstream 100 bases:

>100_bases
AAAAAGCCGCTGACGCGGCCTTTTTCTCGCGGCGGACAATCCGCTCAGCCCGCCTTCAGCACGCGCTGGCGGCGGCTTTC
CGACAACACCATGCCGGACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDIRALRYFVELVKCQSFTRAADALFVTQPTISKMVKQLEEELDMPLILREGRSFRLTDAGRVTYERGLDVLSAMNQLKH
ELADLASLSHGELVVGLPPMVGVAFFAPVVSRYRRRYPQIELKMVEDGALAIENRIKSGELEIGVAVLPVDSQLFEHYSV
VRDPLCLVAPAGSRWRDKSLVQLADIADQPLVLYPDDFTLSRRVGDAFRELGKTPNIVGRSAHWDFIVELVAANLGVTLL
PRSIVERLDRQLFDVVPLYDHKLYWHLALIWQRGGYLSHAARAWLALTRETLGGQD

Sequences:

>Translated_296_residues
MDIRALRYFVELVKCQSFTRAADALFVTQPTISKMVKQLEEELDMPLILREGRSFRLTDAGRVTYERGLDVLSAMNQLKH
ELADLASLSHGELVVGLPPMVGVAFFAPVVSRYRRRYPQIELKMVEDGALAIENRIKSGELEIGVAVLPVDSQLFEHYSV
VRDPLCLVAPAGSRWRDKSLVQLADIADQPLVLYPDDFTLSRRVGDAFRELGKTPNIVGRSAHWDFIVELVAANLGVTLL
PRSIVERLDRQLFDVVPLYDHKLYWHLALIWQRGGYLSHAARAWLALTRETLGGQD
>Mature_296_residues
MDIRALRYFVELVKCQSFTRAADALFVTQPTISKMVKQLEEELDMPLILREGRSFRLTDAGRVTYERGLDVLSAMNQLKH
ELADLASLSHGELVVGLPPMVGVAFFAPVVSRYRRRYPQIELKMVEDGALAIENRIKSGELEIGVAVLPVDSQLFEHYSV
VRDPLCLVAPAGSRWRDKSLVQLADIADQPLVLYPDDFTLSRRVGDAFRELGKTPNIVGRSAHWDFIVELVAANLGVTLL
PRSIVERLDRQLFDVVPLYDHKLYWHLALIWQRGGYLSHAARAWLALTRETLGGQD

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=30.2816901408451, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=28.2157676348548, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=26.0135135135135, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=27.5510204081633, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=26.3565891472868, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790208, Length=284, Percent_Identity=26.7605633802817, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789506, Length=293, Percent_Identity=21.160409556314, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787530, Length=273, Percent_Identity=25.6410256410256, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=22.4489795918367, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787806, Length=275, Percent_Identity=20.3636363636364, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=25.5172413793103, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788296, Length=285, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693193, Length=122, Percent_Identity=30.327868852459, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=23.5772357723577, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788748, Length=241, Percent_Identity=23.2365145228216, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=123, Percent_Identity=34.9593495934959, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=22.568093385214, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=120, Percent_Identity=30.8333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789440, Length=119, Percent_Identity=27.7310924369748, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33434; Mature: 33434

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRALRYFVELVKCQSFTRAADALFVTQPTISKMVKQLEEELDMPLILREGRSFRLTDA
CCHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHCCCCEEEECCCCEEECCC
GRVTYERGLDVLSAMNQLKHELADLASLSHGELVVGLPPMVGVAFFAPVVSRYRRRYPQI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCE
ELKMVEDGALAIENRIKSGELEIGVAVLPVDSQLFEHYSVVRDPLCLVAPAGSRWRDKSL
EEEEECCCHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEECCCCCCCHHHH
VQLADIADQPLVLYPDDFTLSRRVGDAFRELGKTPNIVGRSAHWDFIVELVAANLGVTLL
HHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
PRSIVERLDRQLFDVVPLYDHKLYWHLALIWQRGGYLSHAARAWLALTRETLGGQD
HHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDIRALRYFVELVKCQSFTRAADALFVTQPTISKMVKQLEEELDMPLILREGRSFRLTDA
CCHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHCCCCEEEECCCCEEECCC
GRVTYERGLDVLSAMNQLKHELADLASLSHGELVVGLPPMVGVAFFAPVVSRYRRRYPQI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCE
ELKMVEDGALAIENRIKSGELEIGVAVLPVDSQLFEHYSVVRDPLCLVAPAGSRWRDKSL
EEEEECCCHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEECCCCCCCHHHH
VQLADIADQPLVLYPDDFTLSRRVGDAFRELGKTPNIVGRSAHWDFIVELVAANLGVTLL
HHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
PRSIVERLDRQLFDVVPLYDHKLYWHLALIWQRGGYLSHAARAWLALTRETLGGQD
HHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]