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The map label for this gene is fliA1 [H]

Identifier: 34496477

GI number: 34496477

Start: 1066169

End: 1066915

Strand: Reverse

Name: fliA1 [H]

Synonym: CV_1022

Alternate gene names: 34496477

Gene position: 1066915-1066169 (Counterclockwise)

Preceding gene: 34496478

Following gene: 34496476

Centisome position: 22.46

GC content: 66.4

Gene sequence:

>747_bases
ATGACCTTTCCGTCCTTCCGCCGATCGGGCTACGCGGCAGCGGCCCCCAGCGTCGAGATCGACGTGGAAGCCTATGCCCC
GCTGGTGCGCAAGCTGGCCAGCATCATGATCGCGCGGCTGCCGGCGTCGGTGGAGCTGGGCGATCTGGTCCAGGTCGGCA
TGATCGGCCTGATCGAGGCCGCCCGCCAGTTCGATCCGGCGCAGGGGGTGCAGTTCGAGACCTTCGCCAGCCAGCGCATC
CGCGGCGCGATGCTGGACGAGCTGCGCCGCGAAGACTGGCTGCCGCGCCAGGCGCGCCGCAACTCGCGCCAGATCGAGGA
AGCGATTTCCAAGCTGGAACAGAGGCTGGGCCGCGCGCCGCTGGAGTCGGAAATCGCCGCGGAAATGGGCGTGGAGCTGG
AGCAATACCAGGACATGCTGGGAGACTGCAAGGGCACCACGCTGCTGCATTTCGAAGACTTCGGCGGCGGCGACGACGAG
GGCAGCCACAATCCGGTGACCAATATCGCCGACGACGACTCCCCGGATCCGCTGGCCGCCCTGTCCGACGACAGTTTCCG
CCGGCAGCTGGTCAGCGCGATCAAGTTCCTGCCTGAGCGCGACCAACTGGTGATGGCGTTGTATTACGAGCAGGAGCTGA
ACCTGAAGGAAATCGGCGCGGTGCTCGGCGTCACCGAGTCCCGCGTCTGCCAACTGCACAGCCAGGCCATCGCCCGTCTG
CGCGCAAAACTGAAAGACTGGCTGTAG

Upstream 100 bases:

>100_bases
AATGGGACGCGCCGGAAACCAGCCGCGACAGTACCGGATACATGGACTTGCTGATTGCCGCCTCGCGCGATTGGGCCGAG
GCGGATGGAGCCAAACCCTT

Downstream 100 bases:

>100_bases
GAGCCTGTGATCTTTTTACGAGCAAGAGATCGTAAACAGGCTCTAAGGGAGGGCCCGGCAACGGGTCTCAGACCAGATCG
ATAACGGAACCTTCTTGTGG

Product: RNA polymerase sigma factor for flagellar operon

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTFPSFRRSGYAAAAPSVEIDVEAYAPLVRKLASIMIARLPASVELGDLVQVGMIGLIEAARQFDPAQGVQFETFASQRI
RGAMLDELRREDWLPRQARRNSRQIEEAISKLEQRLGRAPLESEIAAEMGVELEQYQDMLGDCKGTTLLHFEDFGGGDDE
GSHNPVTNIADDDSPDPLAALSDDSFRRQLVSAIKFLPERDQLVMALYYEQELNLKEIGAVLGVTESRVCQLHSQAIARL
RAKLKDWL

Sequences:

>Translated_248_residues
MTFPSFRRSGYAAAAPSVEIDVEAYAPLVRKLASIMIARLPASVELGDLVQVGMIGLIEAARQFDPAQGVQFETFASQRI
RGAMLDELRREDWLPRQARRNSRQIEEAISKLEQRLGRAPLESEIAAEMGVELEQYQDMLGDCKGTTLLHFEDFGGGDDE
GSHNPVTNIADDDSPDPLAALSDDSFRRQLVSAIKFLPERDQLVMALYYEQELNLKEIGAVLGVTESRVCQLHSQAIARL
RAKLKDWL
>Mature_247_residues
TFPSFRRSGYAAAAPSVEIDVEAYAPLVRKLASIMIARLPASVELGDLVQVGMIGLIEAARQFDPAQGVQFETFASQRIR
GAMLDELRREDWLPRQARRNSRQIEEAISKLEQRLGRAPLESEIAAEMGVELEQYQDMLGDCKGTTLLHFEDFGGGDDEG
SHNPVTNIADDDSPDPLAALSDDSFRRQLVSAIKFLPERDQLVMALYYEQELNLKEIGAVLGVTESRVCQLHSQAIARLR
AKLKDWL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=222, Percent_Identity=50.4504504504504, Blast_Score=217, Evalue=5e-58,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=27.7777777777778, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27647; Mature: 27516

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPSFRRSGYAAAAPSVEIDVEAYAPLVRKLASIMIARLPASVELGDLVQVGMIGLIEA
CCCCCHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ARQFDPAQGVQFETFASQRIRGAMLDELRREDWLPRQARRNSRQIEEAISKLEQRLGRAP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LESEIAAEMGVELEQYQDMLGDCKGTTLLHFEDFGGGDDEGSHNPVTNIADDDSPDPLAA
CHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
LSDDSFRRQLVSAIKFLPERDQLVMALYYEQELNLKEIGAVLGVTESRVCQLHSQAIARL
CCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHH
RAKLKDWL
HHHHHHCC
>Mature Secondary Structure 
TFPSFRRSGYAAAAPSVEIDVEAYAPLVRKLASIMIARLPASVELGDLVQVGMIGLIEA
CCCCHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ARQFDPAQGVQFETFASQRIRGAMLDELRREDWLPRQARRNSRQIEEAISKLEQRLGRAP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LESEIAAEMGVELEQYQDMLGDCKGTTLLHFEDFGGGDDEGSHNPVTNIADDDSPDPLAA
CHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
LSDDSFRRQLVSAIKFLPERDQLVMALYYEQELNLKEIGAVLGVTESRVCQLHSQAIARL
CCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHH
RAKLKDWL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]