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The map label for this gene is htrB [H]

Identifier: 34496417

GI number: 34496417

Start: 994586

End: 995482

Strand: Reverse

Name: htrB [H]

Synonym: CV_0962

Alternate gene names: 34496417

Gene position: 995482-994586 (Counterclockwise)

Preceding gene: 34496419

Following gene: 34496416

Centisome position: 20.95

GC content: 65.11

Gene sequence:

>897_bases
ATGTCCAAGCTAGTCCAATTGCTACTGACCTTCCTGGCGAGCCTCCCATTATCCTGGCTACAAGGTTTGGGTGCCGCTCT
GGGGCGGCTGACTTATCTCGCGTCCCCGCGGTTCGCGGCGCGGCTGCGGGAGAATCTGAGTTCTAGTAAAATCTGCCAAA
ACTATCAGCTTTTGCCACAGCAAGTCAAACTGGCGTCGGCGGAAACCGGCAAGGGCGCGCTGGAGCTGGCCATCGCCTGG
TGCCGCAGCCCGGAAGCCATCGCCGCCATGGTCAAGCAGTGCCACGGCTGGGAACACGTCGAACAGGCTCTGGCCCAGGG
TAAGGGCATCGTCTTCGTCACGCCGCACCTGGGCAGCTACGACATCGCCGGGCGCTACATCAGTTCGCGCCTGCCGTTTC
CGCTGACCGCGATGTACCGCCCGCCGAAGCTGCAATGGCTGGAACCGGTGATGCAGGCCGGCCGCGCGCGCGGCAAGGGC
CGCACCGCGCCGGCCACCGCGGCCGGCGTGCGTGTGCTGATGAAGGCGCTGAAATCCGGCGAGGCCACCATCATCCTGCC
CGATCAGGTGCCCGGCAATGGCGAGGGCGTATGGGCGCCCTTCTTCGGCCGGCCCGCCTACACGATGACGCTGGTGCCCC
GCCTGGCGCAAATGAACAAGGTGGCCACCCTGTTCTTCGTCGGCGAGCGCCTGCCCGGCGGCCAGGGTTTCGTCGTGCAC
ATCGAACCGCTGGCCGAACCCTTCACCGGTGACAAGGAAGCCGATTGCGCGCGGATGAACGCGCAGGTGGAAAACCTGAT
CCGCCGTTTTCCGAGCCAGTATCTGTGGAGCTACAACCGCTACAAATGCCCGGCAGGCGTCGCCCGGCCGGACGCAGAAC
AACAAGGACACGCATGA

Upstream 100 bases:

>100_bases
CGAGGTAAACAGGTATTCACTCATTTATTATGCGTTCCCAAAAAATACAAAACCCTGTGATGGTAGAATAGGCCGCTTTC
AGACCAAGACCGCTCAGCTT

Downstream 100 bases:

>100_bases
AATTCGCTTTCGCCCTGCTATGGCTGATCCGCCTGCTGCCGATGCGGGTCATCGGCCTGCTCGCCGCCGGCCTCGGCAAC
CTCGCCTATCTGCTGGCGCG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSKLVQLLLTFLASLPLSWLQGLGAALGRLTYLASPRFAARLRENLSSSKICQNYQLLPQQVKLASAETGKGALELAIAW
CRSPEAIAAMVKQCHGWEHVEQALAQGKGIVFVTPHLGSYDIAGRYISSRLPFPLTAMYRPPKLQWLEPVMQAGRARGKG
RTAPATAAGVRVLMKALKSGEATIILPDQVPGNGEGVWAPFFGRPAYTMTLVPRLAQMNKVATLFFVGERLPGGQGFVVH
IEPLAEPFTGDKEADCARMNAQVENLIRRFPSQYLWSYNRYKCPAGVARPDAEQQGHA

Sequences:

>Translated_298_residues
MSKLVQLLLTFLASLPLSWLQGLGAALGRLTYLASPRFAARLRENLSSSKICQNYQLLPQQVKLASAETGKGALELAIAW
CRSPEAIAAMVKQCHGWEHVEQALAQGKGIVFVTPHLGSYDIAGRYISSRLPFPLTAMYRPPKLQWLEPVMQAGRARGKG
RTAPATAAGVRVLMKALKSGEATIILPDQVPGNGEGVWAPFFGRPAYTMTLVPRLAQMNKVATLFFVGERLPGGQGFVVH
IEPLAEPFTGDKEADCARMNAQVENLIRRFPSQYLWSYNRYKCPAGVARPDAEQQGHA
>Mature_297_residues
SKLVQLLLTFLASLPLSWLQGLGAALGRLTYLASPRFAARLRENLSSSKICQNYQLLPQQVKLASAETGKGALELAIAWC
RSPEAIAAMVKQCHGWEHVEQALAQGKGIVFVTPHLGSYDIAGRYISSRLPFPLTAMYRPPKLQWLEPVMQAGRARGKGR
TAPATAAGVRVLMKALKSGEATIILPDQVPGNGEGVWAPFFGRPAYTMTLVPRLAQMNKVATLFFVGERLPGGQGFVVHI
EPLAEPFTGDKEADCARMNAQVENLIRRFPSQYLWSYNRYKCPAGVARPDAEQQGHA

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=286, Percent_Identity=29.3706293706294, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87082094, Length=256, Percent_Identity=29.296875, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 32598; Mature: 32467

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVQLLLTFLASLPLSWLQGLGAALGRLTYLASPRFAARLRENLSSSKICQNYQLLPQ
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHH
QVKLASAETGKGALELAIAWCRSPEAIAAMVKQCHGWEHVEQALAQGKGIVFVTPHLGSY
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCC
DIAGRYISSRLPFPLTAMYRPPKLQWLEPVMQAGRARGKGRTAPATAAGVRVLMKALKSG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
EATIILPDQVPGNGEGVWAPFFGRPAYTMTLVPRLAQMNKVATLFFVGERLPGGQGFVVH
CEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEE
IEPLAEPFTGDKEADCARMNAQVENLIRRFPSQYLWSYNRYKCPAGVARPDAEQQGHA
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKLVQLLLTFLASLPLSWLQGLGAALGRLTYLASPRFAARLRENLSSSKICQNYQLLPQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHH
QVKLASAETGKGALELAIAWCRSPEAIAAMVKQCHGWEHVEQALAQGKGIVFVTPHLGSY
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCC
DIAGRYISSRLPFPLTAMYRPPKLQWLEPVMQAGRARGKGRTAPATAAGVRVLMKALKSG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
EATIILPDQVPGNGEGVWAPFFGRPAYTMTLVPRLAQMNKVATLFFVGERLPGGQGFVVH
CEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEE
IEPLAEPFTGDKEADCARMNAQVENLIRRFPSQYLWSYNRYKCPAGVARPDAEQQGHA
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]