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The map label for this gene is htrB [H]

Identifier: 34496416

GI number: 34496416

Start: 993723

End: 994589

Strand: Reverse

Name: htrB [H]

Synonym: CV_0961

Alternate gene names: 34496416

Gene position: 994589-993723 (Counterclockwise)

Preceding gene: 34496417

Following gene: 34496414

Centisome position: 20.93

GC content: 63.67

Gene sequence:

>867_bases
ATGAAATTCGCTTTCGCCCTGCTATGGCTGATCCGCCTGCTGCCGATGCGGGTCATCGGCCTGCTCGCCGCCGGCCTCGG
CAACCTCGCCTATCTGCTGGCGCGCGGCCGCCGCCGCGTCGGCCTGATCAACCTGAGGCTGTGCTTTCCGGACATGCCCA
TGGCCGAACGCAAGCGGCTGATCCGCAAGAACTGCCAGCACATGATACGGATGGTGCTGGAGTACGGCGTGTGCTGGTGG
AGCTCAGCCAAGCGCATCGACGGCCTGGTCGAGATCAAGAACCTGCATTACGTCACCGATCTGCGCGACAAGGGCGAGGA
CGTGATCCTGTTCTACCCGCACTTCGTCGGCTTCGAGATGTGCGTCTACGCGCTGAACCAGTACATCCCTCTGGTCAGCG
TGTACTCGCACCAGAAGAACGAGGCGCTGGACAAGCAGATCTACGCCGGGCGCCAGCGCTACGACAACGCCTACATCGTC
TCCCGCCAGGAAGGCCTGCGGCCCATCATCAAGGCGATGCGCAAGGACCACGCGCCCTTCCTCTACCTTCCGGATCAGGA
TTTCGGCGTGCGCGATTCGCTGTTCGTCGACTTCTTCGGCGTCAAGGCTGCCACCATCACCGGCATGTCGCGCATCGCCA
AGCTGGCGCGCGCCAAGGTGGTGCCGGCGATCGCCCGCCGCGTCGGCGACCGCTTCGAACTGGAGTTCTACCCGCCGTGG
GACGGCTACCCCAGCGAGGACATCGAGGCCGACACCCGCCGGATGAACGCTTTCCTGGAAGACCGCGTCCGCGAGATACC
GGACCAGTATTTCTGGCTGCACAAGCGCTTCAAGAACCGCCCTCAAGGCGAAACGCGCGTGTACTAA

Upstream 100 bases:

>100_bases
ACCTGATCCGCCGTTTTCCGAGCCAGTATCTGTGGAGCTACAACCGCTACAAATGCCCGGCAGGCGTCGCCCGGCCGGAC
GCAGAACAACAAGGACACGC

Downstream 100 bases:

>100_bases
CGGATACCCCGCACGTTTGCTCAGGGTTCTGCACGCTCAGGCCGCACTCACTCGGCTGGGCTGAGCGATGCAGAACGGTT
GTTCAACTCGGGATCCATAT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKFAFALLWLIRLLPMRVIGLLAAGLGNLAYLLARGRRRVGLINLRLCFPDMPMAERKRLIRKNCQHMIRMVLEYGVCWW
SSAKRIDGLVEIKNLHYVTDLRDKGEDVILFYPHFVGFEMCVYALNQYIPLVSVYSHQKNEALDKQIYAGRQRYDNAYIV
SRQEGLRPIIKAMRKDHAPFLYLPDQDFGVRDSLFVDFFGVKAATITGMSRIAKLARAKVVPAIARRVGDRFELEFYPPW
DGYPSEDIEADTRRMNAFLEDRVREIPDQYFWLHKRFKNRPQGETRVY

Sequences:

>Translated_288_residues
MKFAFALLWLIRLLPMRVIGLLAAGLGNLAYLLARGRRRVGLINLRLCFPDMPMAERKRLIRKNCQHMIRMVLEYGVCWW
SSAKRIDGLVEIKNLHYVTDLRDKGEDVILFYPHFVGFEMCVYALNQYIPLVSVYSHQKNEALDKQIYAGRQRYDNAYIV
SRQEGLRPIIKAMRKDHAPFLYLPDQDFGVRDSLFVDFFGVKAATITGMSRIAKLARAKVVPAIARRVGDRFELEFYPPW
DGYPSEDIEADTRRMNAFLEDRVREIPDQYFWLHKRFKNRPQGETRVY
>Mature_288_residues
MKFAFALLWLIRLLPMRVIGLLAAGLGNLAYLLARGRRRVGLINLRLCFPDMPMAERKRLIRKNCQHMIRMVLEYGVCWW
SSAKRIDGLVEIKNLHYVTDLRDKGEDVILFYPHFVGFEMCVYALNQYIPLVSVYSHQKNEALDKQIYAGRQRYDNAYIV
SRQEGLRPIIKAMRKDHAPFLYLPDQDFGVRDSLFVDFFGVKAATITGMSRIAKLARAKVVPAIARRVGDRFELEFYPPW
DGYPSEDIEADTRRMNAFLEDRVREIPDQYFWLHKRFKNRPQGETRVY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=284, Percent_Identity=33.4507042253521, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI87082094, Length=289, Percent_Identity=28.719723183391, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788161, Length=286, Percent_Identity=31.1188811188811, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33817; Mature: 33817

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAFALLWLIRLLPMRVIGLLAAGLGNLAYLLARGRRRVGLINLRLCFPDMPMAERKRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHH
IRKNCQHMIRMVLEYGVCWWSSAKRIDGLVEIKNLHYVTDLRDKGEDVILFYPHFVGFEM
HHHHHHHHHHHHHHHCCHHCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHH
CVYALNQYIPLVSVYSHQKNEALDKQIYAGRQRYDNAYIVSRQEGLRPIIKAMRKDHAPF
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEECHHCHHHHHHHHHHCCCCE
LYLPDQDFGVRDSLFVDFFGVKAATITGMSRIAKLARAKVVPAIARRVGDRFELEFYPPW
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DGYPSEDIEADTRRMNAFLEDRVREIPDQYFWLHKRFKNRPQGETRVY
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFAFALLWLIRLLPMRVIGLLAAGLGNLAYLLARGRRRVGLINLRLCFPDMPMAERKRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHH
IRKNCQHMIRMVLEYGVCWWSSAKRIDGLVEIKNLHYVTDLRDKGEDVILFYPHFVGFEM
HHHHHHHHHHHHHHHCCHHCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHH
CVYALNQYIPLVSVYSHQKNEALDKQIYAGRQRYDNAYIVSRQEGLRPIIKAMRKDHAPF
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEECHHCHHHHHHHHHHCCCCE
LYLPDQDFGVRDSLFVDFFGVKAATITGMSRIAKLARAKVVPAIARRVGDRFELEFYPPW
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DGYPSEDIEADTRRMNAFLEDRVREIPDQYFWLHKRFKNRPQGETRVY
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]