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The map label for this gene is ispB [H]

Identifier: 34496302

GI number: 34496302

Start: 878869

End: 879798

Strand: Reverse

Name: ispB [H]

Synonym: CV_0847

Alternate gene names: 34496302

Gene position: 879798-878869 (Counterclockwise)

Preceding gene: 34496311

Following gene: 34496300

Centisome position: 18.52

GC content: 64.52

Gene sequence:

>930_bases
ATGCAGACGGTAGACGGCGTGATCCGCGCCCGCCTGCACTCCGACGTCGTCCTGATCCGCCAGGTGGCGGAATACATCAT
CTCCGCCGGGGGCAAGCGCCTGCGCCCCATCCTGACCCTGCTGTCGGGCCGCGCGCTGGGCTACCGGGGCGAGCACCTGT
TTGAACTCGCCGCGATGATAGAGTTCATTCACACCGCCACCCTGCTGCACGACGACGTGGTGGACGAATCAGACCTGCGC
CGCGGCCGCAAGACCGCCAACGCACTGTTCGGCAACGCCGCCAGCGTGCTGGTGGGCGACTTCCTCTACACCCGCGCCTT
CCAGATGATGGTCACCACCCGCAACATGACCATCCTGGAAGTGATGGCCGAGGCGACCAACATCATCGCCGAGGGCGAAG
TGCTGCAATTGCTGAACATCGGCAACACCGACGTCAGCGAAGACGAATACCTGAAGGTCATCCAGTACAAGACCGCCAAG
CTGTTCGAAGCCGCCGCCCGCGTCGGCGCGCTGATCGCCGACGCCTCCGCCGAGCAGGTGCGGGCGATGGCCGACTACGG
CATGTACCTGGGCACCGCCTTCCAGATCATCGACGACGTGCTGGACTACAGCGGCGACGCCGACACCATAGGCAAGAGCC
TGGGCGACGACCTGGCCGAAGGCAAACCCACGCTGCCGCTGATCTACACCATGCGCAACGGCAGTTCGGAAGCCTCCGCC
CTGGTGCGCGACGCGCTGGAAAACGCCAACCGCGACCGTTTCCACGATGTGCTGAATGCGGTACAATCCTGCGGCGCGCT
GGAATATGCCAAGAACGAAGCCGTGAAAATGGCCGAACAGGCCATCGCCTCCTTGCAGAGCATTCCCGATTCGGAAGTCC
GCCAGGCTCTCATCGAGCTGGCCCGCCTGTCAGTGGACCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
CTTTTCTGGTAACATCCAGAGCTTTGGCGTAGCCATCGCAACGATAAAACTGGTTGAAAACGTGTCCACCCCGCTTTTCC
GAACCCTGATAGCCGACGAC

Downstream 100 bases:

>100_bases
CCAAACCAGTTTTCCAAGCGTCTCCGTAGTTAAATGGATATAACGGCCCCCTCCTAAGGGGCAGTTACAGGTTCGATTCC
TGTCGGGGGCGCCACCCACT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MQTVDGVIRARLHSDVVLIRQVAEYIISAGGKRLRPILTLLSGRALGYRGEHLFELAAMIEFIHTATLLHDDVVDESDLR
RGRKTANALFGNAASVLVGDFLYTRAFQMMVTTRNMTILEVMAEATNIIAEGEVLQLLNIGNTDVSEDEYLKVIQYKTAK
LFEAAARVGALIADASAEQVRAMADYGMYLGTAFQIIDDVLDYSGDADTIGKSLGDDLAEGKPTLPLIYTMRNGSSEASA
LVRDALENANRDRFHDVLNAVQSCGALEYAKNEAVKMAEQAIASLQSIPDSEVRQALIELARLSVDRSA

Sequences:

>Translated_309_residues
MQTVDGVIRARLHSDVVLIRQVAEYIISAGGKRLRPILTLLSGRALGYRGEHLFELAAMIEFIHTATLLHDDVVDESDLR
RGRKTANALFGNAASVLVGDFLYTRAFQMMVTTRNMTILEVMAEATNIIAEGEVLQLLNIGNTDVSEDEYLKVIQYKTAK
LFEAAARVGALIADASAEQVRAMADYGMYLGTAFQIIDDVLDYSGDADTIGKSLGDDLAEGKPTLPLIYTMRNGSSEASA
LVRDALENANRDRFHDVLNAVQSCGALEYAKNEAVKMAEQAIASLQSIPDSEVRQALIELARLSVDRSA
>Mature_309_residues
MQTVDGVIRARLHSDVVLIRQVAEYIISAGGKRLRPILTLLSGRALGYRGEHLFELAAMIEFIHTATLLHDDVVDESDLR
RGRKTANALFGNAASVLVGDFLYTRAFQMMVTTRNMTILEVMAEATNIIAEGEVLQLLNIGNTDVSEDEYLKVIQYKTAK
LFEAAARVGALIADASAEQVRAMADYGMYLGTAFQIIDDVLDYSGDADTIGKSLGDDLAEGKPTLPLIYTMRNGSSEASA
LVRDALENANRDRFHDVLNAVQSCGALEYAKNEAVKMAEQAIASLQSIPDSEVRQALIELARLSVDRSA

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=307, Percent_Identity=28.0130293159609, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4758430, Length=265, Percent_Identity=24.5283018867925, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI83700220, Length=265, Percent_Identity=24.5283018867925, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789578, Length=308, Percent_Identity=54.8701298701299, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786623, Length=232, Percent_Identity=35.3448275862069, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17505681, Length=318, Percent_Identity=30.8176100628931, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=287, Percent_Identity=29.616724738676, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=225, Percent_Identity=24.8888888888889, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=294, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365769, Length=261, Percent_Identity=26.0536398467433, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24660002, Length=261, Percent_Identity=26.0536398467433, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 33702; Mature: 33702

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVDGVIRARLHSDVVLIRQVAEYIISAGGKRLRPILTLLSGRALGYRGEHLFELAAMI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EFIHTATLLHDDVVDESDLRRGRKTANALFGNAASVLVGDFLYTRAFQMMVTTRNMTILE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VMAEATNIIAEGEVLQLLNIGNTDVSEDEYLKVIQYKTAKLFEAAARVGALIADASAEQV
HHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RAMADYGMYLGTAFQIIDDVLDYSGDADTIGKSLGDDLAEGKPTLPLIYTMRNGSSEASA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCEEEEEECCCCHHHHH
LVRDALENANRDRFHDVLNAVQSCGALEYAKNEAVKMAEQAIASLQSIPDSEVRQALIEL
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARLSVDRSA
HHHHHCCCC
>Mature Secondary Structure
MQTVDGVIRARLHSDVVLIRQVAEYIISAGGKRLRPILTLLSGRALGYRGEHLFELAAMI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EFIHTATLLHDDVVDESDLRRGRKTANALFGNAASVLVGDFLYTRAFQMMVTTRNMTILE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VMAEATNIIAEGEVLQLLNIGNTDVSEDEYLKVIQYKTAKLFEAAARVGALIADASAEQV
HHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RAMADYGMYLGTAFQIIDDVLDYSGDADTIGKSLGDDLAEGKPTLPLIYTMRNGSSEASA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCEEEEEECCCCHHHHH
LVRDALENANRDRFHDVLNAVQSCGALEYAKNEAVKMAEQAIASLQSIPDSEVRQALIEL
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARLSVDRSA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]