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The map label for this gene is aroK

Identifier: 34496283

GI number: 34496283

Start: 855471

End: 856025

Strand: Reverse

Name: aroK

Synonym: CV_0828

Alternate gene names: 34496283

Gene position: 856025-855471 (Counterclockwise)

Preceding gene: 34496284

Following gene: 34496282

Centisome position: 18.02

GC content: 63.96

Gene sequence:

>555_bases
ATGCCTGCCATGGAGAAACTGGCAGGCAACTTTTTTCTGGTCGGTCTGATGGGCGCGGGCAAGACCACGGTGGGCAGAGC
GCTGGCGCGCCGCACCGGCAAAACGTTCTACGACTCCGACCAGGAGATCGAGGCGCGCACCGGCGTGCGCGTGGCCACCA
TCTTCGACATCGAAGGCGAAATGCGCTTCCGCAACCGCGAGGCCTGCGTGATCCGCGACCTGGCCCAGCAGCGCGACATC
GTGCTCGCCACCGGCGGCGGCGCTGTGCTGCGCGAGGAAAACCGCAAGGTGCTGGCCAGCCACGGCACAGTGATCTACCT
GCGCGCCTCGATAGACGACCTTCTGGCCCGCACCCAGCACGACAAGAACCGTCCGCTGCTGCAGATCGCCGACCCTCGCG
CCAAACTGGAAAGCCTGTTCAACGAGCGCGATCCGTTTTACCGCGAAATCGCCGACATCATCATCGACACCACCCAGCAG
AACGTCAATCTGCTGGTCGGCCGTCTGGTCGACCAGTTGTTGGACTCCCCGCACCATCCCAAGGAAACCGACTAG

Upstream 100 bases:

>100_bases
CGCAGCGAACTGCTGATTTTCATCACGCCCAAAGTGATCAACGACCTGGTCGCCGAGTCTCGCTAAGCGGCCAAGACAGA
TTCGTTAACGATCGGCTACA

Downstream 100 bases:

>100_bases
TGATCACCCTCGACCTGATCCTGCCCGACACCCGATACCCGATACACATTGGCCACGGACTTCTGGAACAGGTCGACCTG
CTGCTACCCCACCTGCCGCT

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MPAMEKLAGNFFLVGLMGAGKTTVGRALARRTGKTFYDSDQEIEARTGVRVATIFDIEGEMRFRNREACVIRDLAQQRDI
VLATGGGAVLREENRKVLASHGTVIYLRASIDDLLARTQHDKNRPLLQIADPRAKLESLFNERDPFYREIADIIIDTTQQ
NVNLLVGRLVDQLLDSPHHPKETD

Sequences:

>Translated_184_residues
MPAMEKLAGNFFLVGLMGAGKTTVGRALARRTGKTFYDSDQEIEARTGVRVATIFDIEGEMRFRNREACVIRDLAQQRDI
VLATGGGAVLREENRKVLASHGTVIYLRASIDDLLARTQHDKNRPLLQIADPRAKLESLFNERDPFYREIADIIIDTTQQ
NVNLLVGRLVDQLLDSPHHPKETD
>Mature_183_residues
PAMEKLAGNFFLVGLMGAGKTTVGRALARRTGKTFYDSDQEIEARTGVRVATIFDIEGEMRFRNREACVIRDLAQQRDIV
LATGGGAVLREENRKVLASHGTVIYLRASIDDLLARTQHDKNRPLLQIADPRAKLESLFNERDPFYREIADIIIDTTQQN
VNLLVGRLVDQLLDSPHHPKETD

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=165, Percent_Identity=52.1212121212121, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1786587, Length=153, Percent_Identity=37.9084967320261, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_CHRVO (Q7NZU3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900498.1
- ProteinModelPortal:   Q7NZU3
- SMR:   Q7NZU3
- GeneID:   2549258
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_0828
- NMPDR:   fig|243365.1.peg.828
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   Q7NZU3
- ProtClustDB:   CLSK2485332
- BioCyc:   CVIO243365:CV_0828-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 20671; Mature: 20540

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 40-40 BINDING 64-64 BINDING 86-86 BINDING 124-124 BINDING 143-143 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAMEKLAGNFFLVGLMGAGKTTVGRALARRTGKTFYDSDQEIEARTGVRVATIFDIEGE
CCCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEEECCCC
MRFRNREACVIRDLAQQRDIVLATGGGAVLREENRKVLASHGTVIYLRASIDDLLARTQH
EEECCCCHHHHHHHHHCCCEEEEECCCEEEECCCCEEEECCCCEEEEECCHHHHHHHHCC
DKNRPLLQIADPRAKLESLFNERDPFYREIADIIIDTTQQNVNLLVGRLVDQLLDSPHHP
CCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
KETD
CCCC
>Mature Secondary Structure 
PAMEKLAGNFFLVGLMGAGKTTVGRALARRTGKTFYDSDQEIEARTGVRVATIFDIEGE
CCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEEECCCC
MRFRNREACVIRDLAQQRDIVLATGGGAVLREENRKVLASHGTVIYLRASIDDLLARTQH
EEECCCCHHHHHHHHHCCCEEEEECCCEEEECCCCEEEECCCCEEEEECCHHHHHHHHCC
DKNRPLLQIADPRAKLESLFNERDPFYREIADIIIDTTQQNVNLLVGRLVDQLLDSPHHP
CCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
KETD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782