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The map label for this gene is rfaQ [H]

Identifier: 34496277

GI number: 34496277

Start: 848717

End: 849790

Strand: Reverse

Name: rfaQ [H]

Synonym: CV_0822

Alternate gene names: 34496277

Gene position: 849790-848717 (Counterclockwise)

Preceding gene: 34496281

Following gene: 34496276

Centisome position: 17.89

GC content: 66.85

Gene sequence:

>1074_bases
ATGCCCCAGACCGAAGCACCCGACCGCATCCTTCTGATCAAGCTGCGCCATCATGGCGACATTCTGCTCACCACCCCGGT
GGCCCGGACGCTGAAATCGCGCTTCCCGCAGTGCGAGATCGACATGCTGGTCTACCGGGAGACCGTCCCGCTGCTGCAAC
ACAACCCCGACATCGCCCGCGTATGGACGCTTGACCGCTCATTGCGCGGCTGGCGCAAGCTGGTCTCGCAGCTGCGCCTG
CTGCTGGCACTGCGCCGGCGGCGCTACCAGACGGTGATCCACCTGAGCGACCAGATGCAGGGGGCGTTTTTCGCCAAGCT
GCTGGCCCGCCGGCACGCGATCGGCTTCGACTACCCGAAACGCCGCAAGAGCCCGTGGCACCGTTTCTTCACGGCCCTAG
CCCCGCTGGCGCCGAGCGACACCCTGCACACTGTCAGCCAGAACCTGCTGGCGCTATCGCCGCTAGGCATCACGCCGACT
GCCGACGAGCAGCGCTGCGTGCTGCCGATCCAGGCATCCGACCGGGAAGCGGTGGCGGCGCTGCTCCGCGAAGCCGGCGT
CAGCGGTCCCTACATCGTCATCCACCCATCCTCCCGCTGGTTCTTCAAATGCTGGGAGGACGATAGATTCGCCGGGCTGG
CGGAAGCGCTGGCATCCGACGGCTGGTCCATCGTTCTCACCGCCGCGCCCGACCCGGCCGAGATGGACATGGTCGCCGCC
ATACAGGGGAAAATCCGCTCCGACCGCGTCGTCTCCGTCGCCGGCCGGCTGACCCTGAACCAGCTGGCGGCCATCATAGA
CGGCGCCAGGATGTTCATCGGCGTGGACTCCGTCCCCATGCACATGGCCGCGGCACTCGGCAAGGACACCGTCGCCCTCT
TCGGCCCCAGCAAGATCCACGAGTGGCATCCCTGGATGACCCGCCACCGGCTGCTGAACGCCGCCGACTATGGCGAACTG
ATCGATCCGGATCAGGTGGACACCTCGACCCGCCAGCGCTACCTGAAAAACATCCCGCTGGAGGCGGCGCTGCGCGCCAG
CCGCGAACTGCTGTCCTCCCCAGCTCAGGAATAA

Upstream 100 bases:

>100_bases
ATCGCCTGATCGTGATGCCGCCGTGGCGTCTACTAAATCGCATCAGCGCGCCCGCCGAGCCTTCCCAGGCGTCGCCCGGG
ACAAACATCCGCACCCTCTT

Downstream 100 bases:

>100_bases
AGCATGCGCCACACCATTCTGTTCATCATCGACGGCCTGCCCGGGGGCGGCGCCGAGCGCGTGGTCATCACGCTGGCCGG
CGAGATGGCCGCCCGCGGCC

Product: lipopolysaccharide glucosyltransferase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPQTEAPDRILLIKLRHHGDILLTTPVARTLKSRFPQCEIDMLVYRETVPLLQHNPDIARVWTLDRSLRGWRKLVSQLRL
LLALRRRRYQTVIHLSDQMQGAFFAKLLARRHAIGFDYPKRRKSPWHRFFTALAPLAPSDTLHTVSQNLLALSPLGITPT
ADEQRCVLPIQASDREAVAALLREAGVSGPYIVIHPSSRWFFKCWEDDRFAGLAEALASDGWSIVLTAAPDPAEMDMVAA
IQGKIRSDRVVSVAGRLTLNQLAAIIDGARMFIGVDSVPMHMAAALGKDTVALFGPSKIHEWHPWMTRHRLLNAADYGEL
IDPDQVDTSTRQRYLKNIPLEAALRASRELLSSPAQE

Sequences:

>Translated_357_residues
MPQTEAPDRILLIKLRHHGDILLTTPVARTLKSRFPQCEIDMLVYRETVPLLQHNPDIARVWTLDRSLRGWRKLVSQLRL
LLALRRRRYQTVIHLSDQMQGAFFAKLLARRHAIGFDYPKRRKSPWHRFFTALAPLAPSDTLHTVSQNLLALSPLGITPT
ADEQRCVLPIQASDREAVAALLREAGVSGPYIVIHPSSRWFFKCWEDDRFAGLAEALASDGWSIVLTAAPDPAEMDMVAA
IQGKIRSDRVVSVAGRLTLNQLAAIIDGARMFIGVDSVPMHMAAALGKDTVALFGPSKIHEWHPWMTRHRLLNAADYGEL
IDPDQVDTSTRQRYLKNIPLEAALRASRELLSSPAQE
>Mature_356_residues
PQTEAPDRILLIKLRHHGDILLTTPVARTLKSRFPQCEIDMLVYRETVPLLQHNPDIARVWTLDRSLRGWRKLVSQLRLL
LALRRRRYQTVIHLSDQMQGAFFAKLLARRHAIGFDYPKRRKSPWHRFFTALAPLAPSDTLHTVSQNLLALSPLGITPTA
DEQRCVLPIQASDREAVAALLREAGVSGPYIVIHPSSRWFFKCWEDDRFAGLAEALASDGWSIVLTAAPDPAEMDMVAAI
QGKIRSDRVVSVAGRLTLNQLAAIIDGARMFIGVDSVPMHMAAALGKDTVALFGPSKIHEWHPWMTRHRLLNAADYGELI
DPDQVDTSTRQRYLKNIPLEAALRASRELLSSPAQE

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=342, Percent_Identity=34.5029239766082, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790050, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 40157; Mature: 40026

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTEAPDRILLIKLRHHGDILLTTPVARTLKSRFPQCEIDMLVYRETVPLLQHNPDIAR
CCCCCCCCEEEEEEEECCCCEEEECHHHHHHHHHCCCCCCHHEEHHHHHHHHHCCCCEEE
VWTLDRSLRGWRKLVSQLRLLLALRRRRYQTVIHLSDQMQGAFFAKLLARRHAIGFDYPK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCCCCCH
RRKSPWHRFFTALAPLAPSDTLHTVSQNLLALSPLGITPTADEQRCVLPIQASDREAVAA
HCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEEEECCCCHHHHHH
LLREAGVSGPYIVIHPSSRWFFKCWEDDRFAGLAEALASDGWSIVLTAAPDPAEMDMVAA
HHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
IQGKIRSDRVVSVAGRLTLNQLAAIIDGARMFIGVDSVPMHMAAALGKDTVALFGPSKIH
HHCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCEEEECCHHHH
EWHPWMTRHRLLNAADYGELIDPDQVDTSTRQRYLKNIPLEAALRASRELLSSPAQE
HCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PQTEAPDRILLIKLRHHGDILLTTPVARTLKSRFPQCEIDMLVYRETVPLLQHNPDIAR
CCCCCCCEEEEEEEECCCCEEEECHHHHHHHHHCCCCCCHHEEHHHHHHHHHCCCCEEE
VWTLDRSLRGWRKLVSQLRLLLALRRRRYQTVIHLSDQMQGAFFAKLLARRHAIGFDYPK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCCCCCH
RRKSPWHRFFTALAPLAPSDTLHTVSQNLLALSPLGITPTADEQRCVLPIQASDREAVAA
HCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEEEECCCCHHHHHH
LLREAGVSGPYIVIHPSSRWFFKCWEDDRFAGLAEALASDGWSIVLTAAPDPAEMDMVAA
HHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
IQGKIRSDRVVSVAGRLTLNQLAAIIDGARMFIGVDSVPMHMAAALGKDTVALFGPSKIH
HHCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCEEEECCHHHH
EWHPWMTRHRLLNAADYGELIDPDQVDTSTRQRYLKNIPLEAALRASRELLSSPAQE
HCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Transferring other glycosyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA