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The map label for this gene is pleC [H]

Identifier: 34495928

GI number: 34495928

Start: 494464

End: 495303

Strand: Reverse

Name: pleC [H]

Synonym: CV_0473

Alternate gene names: 34495928

Gene position: 495303-494464 (Counterclockwise)

Preceding gene: 34495929

Following gene: 34495927

Centisome position: 10.43

GC content: 63.69

Gene sequence:

>840_bases
GTGAATCCCTCGGTGCCCAAGCTCGGCACCATCTGGGTCTACCAGGACATTTCGGCGCAGCGCGCGGTGGAAGAGACGCT
GCGGCATGCCAAGATCCTGGCCGAGGAAACCAGCCGCGCCAAAACCGAGTTCCTGGCCAATATGTCGCACGAGCTGCGCA
CGCCGCTGCACGCCATCCTGGGCTTCACCGAAATGGGCCAGCTGCGCTCCCAGAGCCTGGATGACGCCAAGCTCGAACAA
TACTTCGCCCGCATCAACACCAGCGGCAACCGGCTGCTGACGCTGCTCAACGACCTGCTGGACATGGCCAAGATGGAAGT
GGGCCGGATGGAGTACGCGATCGGCCGCGGCAACCTGACGCAATGCCTGCGCGAGGCCTGCGACGAGATGACGCCGCTGG
CCGCACGCAGCCAGATCCAGCTGAAACTCAACTGCGAACCGGAAATGCTGGCGGCCGATCTGGACCCCTTCCGCATCGGA
CAGGTGATACGCAACCTGCTGTCCAACGCGATCAAGTTCAGCCCCGCCGGCTCGGAAATCCGGATCCAGGCGCAACAGAC
CGAGCGGGACGGCGCCGACTGGATCATGGCGAGCGTCGAGGACCGGGGCCCCGGGATTCCGGAAAAAGAGCTGGAAACCA
TTTTCGACAAATTCATCCAGAGCAGCACCACCAAGACCGGCGCCGGCGGCACCGGCCTGGGCCTGGCCATCAGCCGCGAA
ATCGTGCATGCCCACCGCGGCTGGATCACCGCCGGCAACCTGCCCGGGGGAGGCGCCATCTTCACTTTCGCGATCCCCTG
CCATTTTCAGCTAGAGAAGGAAGACTCCGATGTCTCATAA

Upstream 100 bases:

>100_bases
GCTGTACAGCCTGCTCAACGACGGCAAGGTGGTGCAGACCGAAGTCAAGCTGCGCACCGGCAAGGGGCAGCTGTTCTGGT
GCCGGCTGCACGGCCGCGCG

Downstream 100 bases:

>100_bases
ACTCCTGCTGGTCGACGACGAACCCTTCAACCTGGAATTGATGGCGGAACTACTGCAGGACGCCGGCTATGAAACCCGCC
AGGCGGAAAACGGGGAGATC

Product: sensor histidine kinase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNPSVPKLGTIWVYQDISAQRAVEETLRHAKILAEETSRAKTEFLANMSHELRTPLHAILGFTEMGQLRSQSLDDAKLEQ
YFARINTSGNRLLTLLNDLLDMAKMEVGRMEYAIGRGNLTQCLREACDEMTPLAARSQIQLKLNCEPEMLAADLDPFRIG
QVIRNLLSNAIKFSPAGSEIRIQAQQTERDGADWIMASVEDRGPGIPEKELETIFDKFIQSSTTKTGAGGTGLGLAISRE
IVHAHRGWITAGNLPGGGAIFTFAIPCHFQLEKEDSDVS

Sequences:

>Translated_279_residues
MNPSVPKLGTIWVYQDISAQRAVEETLRHAKILAEETSRAKTEFLANMSHELRTPLHAILGFTEMGQLRSQSLDDAKLEQ
YFARINTSGNRLLTLLNDLLDMAKMEVGRMEYAIGRGNLTQCLREACDEMTPLAARSQIQLKLNCEPEMLAADLDPFRIG
QVIRNLLSNAIKFSPAGSEIRIQAQQTERDGADWIMASVEDRGPGIPEKELETIFDKFIQSSTTKTGAGGTGLGLAISRE
IVHAHRGWITAGNLPGGGAIFTFAIPCHFQLEKEDSDVS
>Mature_279_residues
MNPSVPKLGTIWVYQDISAQRAVEETLRHAKILAEETSRAKTEFLANMSHELRTPLHAILGFTEMGQLRSQSLDDAKLEQ
YFARINTSGNRLLTLLNDLLDMAKMEVGRMEYAIGRGNLTQCLREACDEMTPLAARSQIQLKLNCEPEMLAADLDPFRIG
QVIRNLLSNAIKFSPAGSEIRIQAQQTERDGADWIMASVEDRGPGIPEKELETIFDKFIQSSTTKTGAGGTGLGLAISRE
IVHAHRGWITAGNLPGGGAIFTFAIPCHFQLEKEDSDVS

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=260, Percent_Identity=35.3846153846154, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789149, Length=242, Percent_Identity=35.1239669421488, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081816, Length=242, Percent_Identity=37.1900826446281, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788713, Length=255, Percent_Identity=30.5882352941176, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI48994928, Length=251, Percent_Identity=35.0597609561753, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=33.7448559670782, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788393, Length=241, Percent_Identity=28.2157676348548, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788549, Length=263, Percent_Identity=29.277566539924, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=29.8245614035088, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790346, Length=232, Percent_Identity=29.7413793103448, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786783, Length=234, Percent_Identity=26.0683760683761, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790436, Length=249, Percent_Identity=29.718875502008, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790861, Length=243, Percent_Identity=28.3950617283951, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790300, Length=255, Percent_Identity=27.843137254902, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790551, Length=214, Percent_Identity=32.2429906542056, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=27.1929824561404, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789808, Length=252, Percent_Identity=26.1904761904762, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788279, Length=225, Percent_Identity=24, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=238, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787374, Length=231, Percent_Identity=31.6017316017316, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 30799; Mature: 30799

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSVPKLGTIWVYQDISAQRAVEETLRHAKILAEETSRAKTEFLANMSHELRTPLHAIL
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFTEMGQLRSQSLDDAKLEQYFARINTSGNRLLTLLNDLLDMAKMEVGRMEYAIGRGNLT
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHH
QCLREACDEMTPLAARSQIQLKLNCEPEMLAADLDPFRIGQVIRNLLSNAIKFSPAGSEI
HHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEE
RIQAQQTERDGADWIMASVEDRGPGIPEKELETIFDKFIQSSTTKTGAGGTGLGLAISRE
EEEEHHCCCCCCHHEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IVHAHRGWITAGNLPGGGAIFTFAIPCHFQLEKEDSDVS
HHHHHCCEEEECCCCCCCEEEEEEECEEEEECCCCCCCC
>Mature Secondary Structure
MNPSVPKLGTIWVYQDISAQRAVEETLRHAKILAEETSRAKTEFLANMSHELRTPLHAIL
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFTEMGQLRSQSLDDAKLEQYFARINTSGNRLLTLLNDLLDMAKMEVGRMEYAIGRGNLT
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHH
QCLREACDEMTPLAARSQIQLKLNCEPEMLAADLDPFRIGQVIRNLLSNAIKFSPAGSEI
HHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEE
RIQAQQTERDGADWIMASVEDRGPGIPEKELETIFDKFIQSSTTKTGAGGTGLGLAISRE
EEEEHHCCCCCCHHEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IVHAHRGWITAGNLPGGGAIFTFAIPCHFQLEKEDSDVS
HHHHHCCEEEECCCCCCCEEEEEEECEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]