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The map label for this gene is phoB [H]

Identifier: 34495927

GI number: 34495927

Start: 493581

End: 494474

Strand: Reverse

Name: phoB [H]

Synonym: CV_0472

Alternate gene names: 34495927

Gene position: 494474-493581 (Counterclockwise)

Preceding gene: 34495928

Following gene: 34495925

Centisome position: 10.41

GC content: 64.99

Gene sequence:

>894_bases
ATGTCTCATAAACTCCTGCTGGTCGACGACGAACCCTTCAACCTGGAATTGATGGCGGAACTACTGCAGGACGCCGGCTA
TGAAACCCGCCAGGCGGAAAACGGGGAGATCGCCTGGGACATCCTGTCGCAAGAGGGCGAGCAATACTCAACCGTGCTGC
TGGACAAGATGATGCCCGGCATGAGCGGCTTCGACGTGCTGAAGAAGATCAAGGCGGAACCCCGGCTGGAGTTCCTGCCC
GTGATCATGCAGACGGCGATGGGCGCGGCCGCCAGCGTGCAGGAAGGCCTGGCGGCCGGCGCCTTCTACTACCTGACCAA
GCCGTTCTCCCGCGACATGCTGCTGGCCATCGTCGGCGCCGCCGTCGGCCACTGGGACCGCTACGCACGCTTCCGCGAGC
TGGCCAACCTGCACGTCGACGCGCTCAAGCACCTGCGCGCCGCCACCTTCCGCTGCCGCAACCACCGCGAGGCGCAAGCG
ATCACCGCCTTGCTGGCCAAGACCTGCCGGCAGCCGGAGCGCGTGGCCACCGGCCTGTTCGAGCTGCTGGTCAACGCGAT
CGAACACGGCAACCTGGAGATCAGCTACGAGCAGAAAAGCCAGCTGATCGCCAACGGCCGCTGGGAGCAGGAGCTGGAAA
GCCGGCTGCAGGCGCCGGAGTATCTGGACCGCGAAGTCACCGTGGAATTCTCCCACCAGGGCACGCAGCTGCAGTTCGCG
ATCGAGGACATGGGACCGGGCTTCGACTGGGCGTACTACCAGGATGCGGAGCCGTCTTCGCTGATGAACAGCCACGGCCG
CGGCATCCTGATCGCGCGCAAGCTGTCCTTCGACGACCTGCGCTACCAGGGCAAGGGCAACCGCGTGGTCGCGCTGGTCA
ATCAGGAAGACTGA

Upstream 100 bases:

>100_bases
GCCCACCGCGGCTGGATCACCGCCGGCAACCTGCCCGGGGGAGGCGCCATCTTCACTTTCGCGATCCCCTGCCATTTTCA
GCTAGAGAAGGAAGACTCCG

Downstream 100 bases:

>100_bases
CCGACTCGCCGCAGGGGCCGGCCTCGCGCCAGTAACGCGGCTGCTCGCGCCGCAGGCAGCGCCAGGCCAGCGTCTCTCCC
ATTTCCAGCGTCAACGCGCC

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSHKLLLVDDEPFNLELMAELLQDAGYETRQAENGEIAWDILSQEGEQYSTVLLDKMMPGMSGFDVLKKIKAEPRLEFLP
VIMQTAMGAAASVQEGLAAGAFYYLTKPFSRDMLLAIVGAAVGHWDRYARFRELANLHVDALKHLRAATFRCRNHREAQA
ITALLAKTCRQPERVATGLFELLVNAIEHGNLEISYEQKSQLIANGRWEQELESRLQAPEYLDREVTVEFSHQGTQLQFA
IEDMGPGFDWAYYQDAEPSSLMNSHGRGILIARKLSFDDLRYQGKGNRVVALVNQED

Sequences:

>Translated_297_residues
MSHKLLLVDDEPFNLELMAELLQDAGYETRQAENGEIAWDILSQEGEQYSTVLLDKMMPGMSGFDVLKKIKAEPRLEFLP
VIMQTAMGAAASVQEGLAAGAFYYLTKPFSRDMLLAIVGAAVGHWDRYARFRELANLHVDALKHLRAATFRCRNHREAQA
ITALLAKTCRQPERVATGLFELLVNAIEHGNLEISYEQKSQLIANGRWEQELESRLQAPEYLDREVTVEFSHQGTQLQFA
IEDMGPGFDWAYYQDAEPSSLMNSHGRGILIARKLSFDDLRYQGKGNRVVALVNQED
>Mature_296_residues
SHKLLLVDDEPFNLELMAELLQDAGYETRQAENGEIAWDILSQEGEQYSTVLLDKMMPGMSGFDVLKKIKAEPRLEFLPV
IMQTAMGAAASVQEGLAAGAFYYLTKPFSRDMLLAIVGAAVGHWDRYARFRELANLHVDALKHLRAATFRCRNHREAQAI
TALLAKTCRQPERVATGLFELLVNAIEHGNLEISYEQKSQLIANGRWEQELESRLQAPEYLDREVTVEFSHQGTQLQFAI
EDMGPGFDWAYYQDAEPSSLMNSHGRGILIARKLSFDDLRYQGKGNRVVALVNQED

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=29.4871794871795, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082012, Length=141, Percent_Identity=30.4964539007092, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786784, Length=154, Percent_Identity=32.4675324675325, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 33552; Mature: 33420

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKLLLVDDEPFNLELMAELLQDAGYETRQAENGEIAWDILSQEGEQYSTVLLDKMMPG
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCC
MSGFDVLKKIKAEPRLEFLPVIMQTAMGAAASVQEGLAAGAFYYLTKPFSRDMLLAIVGA
CCHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCHHHEECCCCCHHHHHHHHHH
AVGHWDRYARFRELANLHVDALKHLRAATFRCRNHREAQAITALLAKTCRQPERVATGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
ELLVNAIEHGNLEISYEQKSQLIANGRWEQELESRLQAPEYLDREVTVEFSHQGTQLQFA
HHHHHHHHCCCEEEEEHHHHHHHHCCCCHHHHHHHCCCHHHCCCEEEEEECCCCCEEEEE
IEDMGPGFDWAYYQDAEPSSLMNSHGRGILIARKLSFDDLRYQGKGNRVVALVNQED
EECCCCCCCCCEECCCCHHHHHHCCCCEEEEEEECCHHHHEEECCCCEEEEEEECCC
>Mature Secondary Structure 
SHKLLLVDDEPFNLELMAELLQDAGYETRQAENGEIAWDILSQEGEQYSTVLLDKMMPG
CCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCC
MSGFDVLKKIKAEPRLEFLPVIMQTAMGAAASVQEGLAAGAFYYLTKPFSRDMLLAIVGA
CCHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCHHHEECCCCCHHHHHHHHHH
AVGHWDRYARFRELANLHVDALKHLRAATFRCRNHREAQAITALLAKTCRQPERVATGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
ELLVNAIEHGNLEISYEQKSQLIANGRWEQELESRLQAPEYLDREVTVEFSHQGTQLQFA
HHHHHHHHCCCEEEEEHHHHHHHHCCCCHHHHHHHCCCHHHCCCEEEEEECCCCCEEEEE
IEDMGPGFDWAYYQDAEPSSLMNSHGRGILIARKLSFDDLRYQGKGNRVVALVNQED
EECCCCCCCCCEECCCCHHHHHHCCCCEEEEEEECCHHHHEEECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]