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The map label for this gene is mlaE [H]

Identifier: 34495903

GI number: 34495903

Start: 465049

End: 465822

Strand: Reverse

Name: mlaE [H]

Synonym: CV_0448

Alternate gene names: 34495903

Gene position: 465822-465049 (Counterclockwise)

Preceding gene: 34495904

Following gene: 34495902

Centisome position: 9.8

GC content: 68.22

Gene sequence:

>774_bases
CTGACCTCGCCGCTGCGCCGCCTCGGCCACCTCACCGTCAACGCCGTCTGGCGGCTGGGCTTCGCCAGCCGCTTCCTGGC
GGCCATCCTGCTCAACAGCGGCCAGAGCCTGCTGCGGCTGCAGATGACCATACGCGAGATCTACTTCGCCGGCGTGATGT
CGCTGATCATCATCGTGGTGTCCGGCCTGTTCGTCGGCATGGTGCTGGGCCTGCAGGGCTACACCACGCTGGCCAAGTTC
GGCTCCGCCGACGCGCTGGGCGCGATGGTGGCGCTGGCGCTGCTGCGCGAGCTGGGCCCGGTGCTGGCCGCGCTGCTGTT
CGCCAGCCGCGCCGGCAGCGCGATGACCGCGGAAATCGGCCTGATGAAGACCACCGAGCAGCTGGACGCGATGAGCGTGA
TGGCGGTCAATCCGATGGCGCGCGTGATCGCGCCGCGCTTCTGGGCCGGCGTCGTCTCCATGCCCATCCTGGCCGCGCTG
TTCAATGTGGTCGGCATCTTCGGCGGCTACCTGGTCGGCGTGGTGATGATAGGCCTGGACGCCGGGACGTTCTGGTCGCA
GATGCAGGGCAACGTCGACCTGCACTACGACGTGGTCAACGGCATCATCAAGAGCCTGGTGTTCGGCATCGCCGTGACGC
TGATCGCCGTGTTCGAAGGCTACGACGCCACCCCGACCGCCGCCGGCGTGTCGGCCGCCACCACCCGCACCGTGGTCACC
TCGGCGCTGGTGATCCTGGCGCTGGACTTCGTGCTGACCGCCTTCATGTTCTAG

Upstream 100 bases:

>100_bases
ATCAACGGCGAGGCCGACGGCCCGGTGCACTACAATTTCCCCGCCGCCACCTCGCTGGCGGCCGATCTGGGTCTGCAAGG
AGGCGGGCATGCTTGATTTT

Downstream 100 bases:

>100_bases
GAAAAAAACCAATGAAACGCTCTACCATTGATTTGTGGGTCGGCATCTTTGTCGCCCTCGGCATCGCCGCCGTCGTCTTC
CTGTCGCTGAAGGTGGCCAA

Product: ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTSPLRRLGHLTVNAVWRLGFASRFLAAILLNSGQSLLRLQMTIREIYFAGVMSLIIIVVSGLFVGMVLGLQGYTTLAKF
GSADALGAMVALALLRELGPVLAALLFASRAGSAMTAEIGLMKTTEQLDAMSVMAVNPMARVIAPRFWAGVVSMPILAAL
FNVVGIFGGYLVGVVMIGLDAGTFWSQMQGNVDLHYDVVNGIIKSLVFGIAVTLIAVFEGYDATPTAAGVSAATTRTVVT
SALVILALDFVLTAFMF

Sequences:

>Translated_257_residues
MTSPLRRLGHLTVNAVWRLGFASRFLAAILLNSGQSLLRLQMTIREIYFAGVMSLIIIVVSGLFVGMVLGLQGYTTLAKF
GSADALGAMVALALLRELGPVLAALLFASRAGSAMTAEIGLMKTTEQLDAMSVMAVNPMARVIAPRFWAGVVSMPILAAL
FNVVGIFGGYLVGVVMIGLDAGTFWSQMQGNVDLHYDVVNGIIKSLVFGIAVTLIAVFEGYDATPTAAGVSAATTRTVVT
SALVILALDFVLTAFMF
>Mature_256_residues
TSPLRRLGHLTVNAVWRLGFASRFLAAILLNSGQSLLRLQMTIREIYFAGVMSLIIIVVSGLFVGMVLGLQGYTTLAKFG
SADALGAMVALALLRELGPVLAALLFASRAGSAMTAEIGLMKTTEQLDAMSVMAVNPMARVIAPRFWAGVVSMPILAALF
NVVGIFGGYLVGVVMIGLDAGTFWSQMQGNVDLHYDVVNGIIKSLVFGIAVTLIAVFEGYDATPTAAGVSAATTRTVVTS
ALVILALDFVLTAFMF

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=253, Percent_Identity=56.9169960474308, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27099; Mature: 26968

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPLRRLGHLTVNAVWRLGFASRFLAAILLNSGQSLLRLQMTIREIYFAGVMSLIIIVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLFVGMVLGLQGYTTLAKFGSADALGAMVALALLRELGPVLAALLFASRAGSAMTAEIG
HHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LMKTTEQLDAMSVMAVNPMARVIAPRFWAGVVSMPILAALFNVVGIFGGYLVGVVMIGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGTFWSQMQGNVDLHYDVVNGIIKSLVFGIAVTLIAVFEGYDATPTAAGVSAATTRTVVT
CHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SALVILALDFVLTAFMF
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]