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The map label for this gene is pyrG [H]

Identifier: 33864483

GI number: 33864483

Start: 2346450

End: 2348126

Strand: Direct

Name: pyrG [H]

Synonym: PMT2219

Alternate gene names: 33864483

Gene position: 2346450-2348126 (Clockwise)

Preceding gene: 33864482

Following gene: 33864484

Centisome position: 97.33

GC content: 54.98

Gene sequence:

>1677_bases
ATGGCCAAGTTCGTCTTTGTTACCGGTGGAGTCGTATCAAGCATCGGCAAGGGAATTGTGGCCGCGAGCCTTGGTCGCCT
ACTCAAATCGCGTGGCTACAGCGTCTCGATCCTGAAGCTGGATCCCTACCTCAATGTGGACCCAGGCACCATGAGTCCAT
TTCAACATGGTGAAGTGTTCGTTACCGAAGACGGCGCCGAAACCGACTTGGATCTTGGCCACTACGAGCGCTTTACTGAC
ACAGCCATGTCACGCCTCAATAGCGTAACCACTGGCTCAATCTATCAAGCAGTCATCAATAAGGAACGGCGCGGCGACTA
CAACGGCGGCACTGTGCAAGTGATCCCCCACATCACTGGTGAGATTCGCGAACGCATCCACCGAGTAGCAGCCAATAGTG
GCGCAGATGTCGTGATCACAGAAATCGGCGGCACTGTCGGAGACATCGAATCTCTGCCCTTCCTCGAAGCAATCCGTGAA
TTCCGTGGTGACGTAGGACGCAACGACCTCGCCTACATCCATGTGACCCTACTCCCGTTCATCGGCACATCCGGTGAACT
CAAAACCAAGCCCACACAGCACTCCGTCAAGGAACTGCGCGCAATTGGCATACAACCAGATGTACTGGTTTGCCGCAGCG
ACCGCCCAATCAATGAAGACCTAAAGAGCAAAATTGGAGGCTTCTGCGGGGTACCAAACCGGGCTGTCATCACTGCTCTT
GATGCCGACAGCATTTATGCCGTACCCATCTCACTAGAGGAAGAGGGCCTCTGTCTAGAAATGCTGGATGTACTCAACCT
GACTGACCATGACAGCGACATGAAAAGCTGGGTTGAGCTGGTTCATAAGCTGCGCAACCCAGGTCCCGCTGTGAAGGTGG
CCCTAGTCGGAAAATATGTGCAACTCAACGACGCCTACCTTTCAGTAGTAGAAGCCCTGCGCCATGCCTGCCTCACCCAA
GACGCCTCCCTCGAGCTGTCTTGGGTCTGCGCCGAGCAGATCGAAACGCATGGTCCAGAAAACCTACTCAAAGGAATGGA
TGCTGTTGTAGTTCCTGGAGGCTTCGGTAACCGCGGCGTAGATGGCAAAGTTGCTGCCATTCGCTGGGCGAGAGAACAAC
GAGTGCCATTCCTAGGGCTTTGTCTAGGCATGCAATGCGCTGTGATCGAGTGGGCACGCAACCAGGCAGGACTCACTGGA
GCCAGTAGTTCCGAGCTGGAGCCCGACACCAACCATCCTGTTATCCACCTCCTGCCAGAGCAACAAGACGTAGTCGATCT
AGGCGGCACAATGAGGCTTGGTGTCTATCCATGCCGCATCGCACCAGACACGTTGGCACAGAAGCTTTATGGCGAACAGG
TTGTCTACGAGCGCCACCGCCATCGCTATGAATTCAACAACTCCTACCGCAACCTATTTATTGAATCCGGCTACACCATC
AGCGGTAGCTCACCCGATGGACGCCTCGTAGAACTGATCGAGCTGAAAGGTCATCCCTTCTTCACAGCCTGCCAATACCA
CCCTGAATTTCTCTCCCGACCCGGAAAACCCCATCCCCTCTTCCGGGGATTGATTGAGGCAGCTCAACTACGGCTACCAG
CCTCACCTGACGAAGCCCTACGCCGACAAAGCCAGACCAACATCTCCGCTCAGGAGCAACCCAGCCGGATTGGATGA

Upstream 100 bases:

>100_bases
ACCCAGCGGCTTGAGATTCACGAAAAAACCGCCTGGATGCTGCGCAGCCTGCTGGAAGCATAAGAAAGCCGAGCTAACTT
AAAGAATCAGCCTGAAATTC

Downstream 100 bases:

>100_bases
CCGGCAATCTGCCGGTAGTAGAAACCTTCCATTCCCTACAAGGAGAAGGAGCCCATGTCGGTCGCAGCGCCTTCTTCATC
AGGCTCGCCGGCTGTGATGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MAKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTD
TAMSRLNSVTTGSIYQAVINKERRGDYNGGTVQVIPHITGEIRERIHRVAANSGADVVITEIGGTVGDIESLPFLEAIRE
FRGDVGRNDLAYIHVTLLPFIGTSGELKTKPTQHSVKELRAIGIQPDVLVCRSDRPINEDLKSKIGGFCGVPNRAVITAL
DADSIYAVPISLEEEGLCLEMLDVLNLTDHDSDMKSWVELVHKLRNPGPAVKVALVGKYVQLNDAYLSVVEALRHACLTQ
DASLELSWVCAEQIETHGPENLLKGMDAVVVPGGFGNRGVDGKVAAIRWAREQRVPFLGLCLGMQCAVIEWARNQAGLTG
ASSSELEPDTNHPVIHLLPEQQDVVDLGGTMRLGVYPCRIAPDTLAQKLYGEQVVYERHRHRYEFNNSYRNLFIESGYTI
SGSSPDGRLVELIELKGHPFFTACQYHPEFLSRPGKPHPLFRGLIEAAQLRLPASPDEALRRQSQTNISAQEQPSRIG

Sequences:

>Translated_558_residues
MAKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTD
TAMSRLNSVTTGSIYQAVINKERRGDYNGGTVQVIPHITGEIRERIHRVAANSGADVVITEIGGTVGDIESLPFLEAIRE
FRGDVGRNDLAYIHVTLLPFIGTSGELKTKPTQHSVKELRAIGIQPDVLVCRSDRPINEDLKSKIGGFCGVPNRAVITAL
DADSIYAVPISLEEEGLCLEMLDVLNLTDHDSDMKSWVELVHKLRNPGPAVKVALVGKYVQLNDAYLSVVEALRHACLTQ
DASLELSWVCAEQIETHGPENLLKGMDAVVVPGGFGNRGVDGKVAAIRWAREQRVPFLGLCLGMQCAVIEWARNQAGLTG
ASSSELEPDTNHPVIHLLPEQQDVVDLGGTMRLGVYPCRIAPDTLAQKLYGEQVVYERHRHRYEFNNSYRNLFIESGYTI
SGSSPDGRLVELIELKGHPFFTACQYHPEFLSRPGKPHPLFRGLIEAAQLRLPASPDEALRRQSQTNISAQEQPSRIG
>Mature_557_residues
AKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDT
AMSRLNSVTTGSIYQAVINKERRGDYNGGTVQVIPHITGEIRERIHRVAANSGADVVITEIGGTVGDIESLPFLEAIREF
RGDVGRNDLAYIHVTLLPFIGTSGELKTKPTQHSVKELRAIGIQPDVLVCRSDRPINEDLKSKIGGFCGVPNRAVITALD
ADSIYAVPISLEEEGLCLEMLDVLNLTDHDSDMKSWVELVHKLRNPGPAVKVALVGKYVQLNDAYLSVVEALRHACLTQD
ASLELSWVCAEQIETHGPENLLKGMDAVVVPGGFGNRGVDGKVAAIRWAREQRVPFLGLCLGMQCAVIEWARNQAGLTGA
SSSELEPDTNHPVIHLLPEQQDVVDLGGTMRLGVYPCRIAPDTLAQKLYGEQVVYERHRHRYEFNNSYRNLFIESGYTIS
GSSPDGRLVELIELKGHPFFTACQYHPEFLSRPGKPHPLFRGLIEAAQLRLPASPDEALRRQSQTNISAQEQPSRIG

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=46.4609800362976, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI28559085, Length=568, Percent_Identity=44.3661971830986, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=568, Percent_Identity=44.3661971830986, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=568, Percent_Identity=44.3661971830986, Blast_Score=468, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=52.8864059590317, Blast_Score=573, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=39.2384105960265, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322563, Length=572, Percent_Identity=44.5804195804196, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319432, Length=559, Percent_Identity=42.9338103756708, Blast_Score=449, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24664469, Length=581, Percent_Identity=45.4388984509466, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI21357815, Length=526, Percent_Identity=44.2965779467681, Blast_Score=418, Evalue=1e-117,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 61149; Mature: 61018

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDTAMSRLNSVTTGSIYQAVINKERRGDYNGGTVQVIPHITG
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHH
EIRERIHRVAANSGADVVITEIGGTVGDIESLPFLEAIREFRGDVGRNDLAYIHVTLLPF
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEC
IGTSGELKTKPTQHSVKELRAIGIQPDVLVCRSDRPINEDLKSKIGGFCGVPNRAVITAL
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
DADSIYAVPISLEEEGLCLEMLDVLNLTDHDSDMKSWVELVHKLRNPGPAVKVALVGKYV
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECEE
QLNDAYLSVVEALRHACLTQDASLELSWVCAEQIETHGPENLLKGMDAVVVPGGFGNRGV
EECHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCHHHHHCCCCEEEECCCCCCCCC
DGKVAAIRWAREQRVPFLGLCLGMQCAVIEWARNQAGLTGASSSELEPDTNHPVIHLLPE
CCCCCEEEEHHHHCCCEEHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCC
QQDVVDLGGTMRLGVYPCRIAPDTLAQKLYGEQVVYERHRHRYEFNNSYRNLFIESGYTI
CCCHHHCCCEEEECEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEE
SGSSPDGRLVELIELKGHPFFTACQYHPEFLSRPGKPHPLFRGLIEAAQLRLPASPDEAL
ECCCCCCCEEEEEEECCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
RRQSQTNISAQEQPSRIG
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDTAMSRLNSVTTGSIYQAVINKERRGDYNGGTVQVIPHITG
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHH
EIRERIHRVAANSGADVVITEIGGTVGDIESLPFLEAIREFRGDVGRNDLAYIHVTLLPF
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEC
IGTSGELKTKPTQHSVKELRAIGIQPDVLVCRSDRPINEDLKSKIGGFCGVPNRAVITAL
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
DADSIYAVPISLEEEGLCLEMLDVLNLTDHDSDMKSWVELVHKLRNPGPAVKVALVGKYV
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECEE
QLNDAYLSVVEALRHACLTQDASLELSWVCAEQIETHGPENLLKGMDAVVVPGGFGNRGV
EECHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCHHHHHCCCCEEEECCCCCCCCC
DGKVAAIRWAREQRVPFLGLCLGMQCAVIEWARNQAGLTGASSSELEPDTNHPVIHLLPE
CCCCCEEEEHHHHCCCEEHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCC
QQDVVDLGGTMRLGVYPCRIAPDTLAQKLYGEQVVYERHRHRYEFNNSYRNLFIESGYTI
CCCHHHCCCEEEECEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEE
SGSSPDGRLVELIELKGHPFFTACQYHPEFLSRPGKPHPLFRGLIEAAQLRLPASPDEAL
ECCCCCCCEEEEEEECCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
RRQSQTNISAQEQPSRIG
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA