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The map label for this gene is dnaB [H]

Identifier: 33864440

GI number: 33864440

Start: 2302282

End: 2303700

Strand: Direct

Name: dnaB [H]

Synonym: PMT2176

Alternate gene names: 33864440

Gene position: 2302282-2303700 (Clockwise)

Preceding gene: 33864439

Following gene: 33864441

Centisome position: 95.5

GC content: 56.24

Gene sequence:

>1419_bases
ATGGTGAGCGCCCCCCTGCCAAACAACGGCGATACCGCCGCAGAGGGGTCCCGTCCCTTCAGGAAAGCCTCTTCTACATC
TCGAGAACCGAGCTTTGAAGCCCTGCCAGACTCCGTGCCTCCTCAAAATCTGGAGGCAGAGGAAGCTGTTCTAGGAGGCA
TCCTTCTTGATCCTGATGCCATCGGCAGAGTGGTCGATGTGCTCCAACCTGAGGCTTTTTATCTCAACGCCCATCGAGAG
ATCTTCCGCACAGCCCTGATGCTTCACAGCCAGGGCAAACCCACCGACCTCACAGCAATGAGCGCCTGGTTGGCCGACAC
AGGGTCCCTGGAGAAGGTAGGTGGCAGCAACCGTCTGATCGAACTTGTAGAGCGCGTTGCCTCCACTGCATCAATCGAGC
AAGTGGCCCGTTTGGTGATGGACAAATTCCTACGGCGACAGTTGATTCGCTCAGGCAACGAAGTGATTCAACTCGGCTTT
GACCAGGGCCTACCGATGGAACAGGTTCTCGACAAAGCAGAACAAACAATTTTTGCGATCAGCCAGGAAAAGCCATCCAA
AGGTCTCACCCCCACAGCAGAAATCCTTACCAGCACCTTCAACGAGATCGAAAGCCGCTCACTAGGTACGTCCGTCGCAG
GCATCCCTGTGAACTTCTACGACCTCGATGCCATGACCCAGGGTCTGCAGCGCAGTGACCTGATCATCGTGGCTGGCCGC
CCAGCCATGGGCAAAACCTCCATGGTGCTCAATCTGACCAAAAACGTTGCTCAACTCCACAACCTGCCTGTGTGCTTGTT
CAGCCTGGAGATGAGCAAGGAACAACTCACCTATCGCCTCCTTTCAATGGAGGTTGGCATTGAGAGCAGTCGACTGAGAA
CAGGCCGTTTACGGCAGGAAGAGTGGCCTCTTCTGGGCCAGGGCATCAACACCCTTGGCCAGCTACCGATCTACATCGAT
GACAAACCCAATTCAGGCGTCCTGGAGATGCGTTCTCTCTCCAGGCGGCTCATGGCCGAACAAGGCAAAGAACTCGGACT
CATCGTGATCGACTACCTACAACTGATGGAGGGCTCCACGCCTGATAACCGTGTTCAGGAACTCTCCCGGATTACCCGCG
GCCTTAAGGGCATGGCCAGGGAACTGAACGTACCTGTGATTGCCTTATCACAGCTCAGCCGTGGGGTGGAATCCCGCACC
AACAAACGCCCAATGCTCAGCGACCTGCGCGAGTCAGGCTCAATCGAACAGGACGCTGACCTGGTGCTGATGATCTACCG
CGACGAGTACTACAACCCAGAAACCCCCGACCGCGGCATCACCGAAGTCATCGTGACCAAGCACCGCAACGGCCCTGTCG
GCACAGTAAAACTACTGTTCGAGCCTCAATTCACACGGTTCCGCAACCTGGCGGCCTAA

Upstream 100 bases:

>100_bases
TCAACGCCGAAATCAACCTCGAAGTCGTCAGCTACTGATTGCTGGCAAGGCTTACAAAACAAGCCAAAGTAGGTCTCCTT
ATAACGTCAGCTCCTCATCC

Downstream 100 bases:

>100_bases
AGCAGCAATACATTGATGATTTCTACTGTTCTCTAGAACGTCACGGGCCCCATGCCCTTCAGTGCTCCCCCAACCGAACA
CTTCGACGTCATCATCGTCG

Product: DnaB replicative helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVSAPLPNNGDTAAEGSRPFRKASSTSREPSFEALPDSVPPQNLEAEEAVLGGILLDPDAIGRVVDVLQPEAFYLNAHRE
IFRTALMLHSQGKPTDLTAMSAWLADTGSLEKVGGSNRLIELVERVASTASIEQVARLVMDKFLRRQLIRSGNEVIQLGF
DQGLPMEQVLDKAEQTIFAISQEKPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
PAMGKTSMVLNLTKNVAQLHNLPVCLFSLEMSKEQLTYRLLSMEVGIESSRLRTGRLRQEEWPLLGQGINTLGQLPIYID
DKPNSGVLEMRSLSRRLMAEQGKELGLIVIDYLQLMEGSTPDNRVQELSRITRGLKGMARELNVPVIALSQLSRGVESRT
NKRPMLSDLRESGSIEQDADLVLMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA

Sequences:

>Translated_472_residues
MVSAPLPNNGDTAAEGSRPFRKASSTSREPSFEALPDSVPPQNLEAEEAVLGGILLDPDAIGRVVDVLQPEAFYLNAHRE
IFRTALMLHSQGKPTDLTAMSAWLADTGSLEKVGGSNRLIELVERVASTASIEQVARLVMDKFLRRQLIRSGNEVIQLGF
DQGLPMEQVLDKAEQTIFAISQEKPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
PAMGKTSMVLNLTKNVAQLHNLPVCLFSLEMSKEQLTYRLLSMEVGIESSRLRTGRLRQEEWPLLGQGINTLGQLPIYID
DKPNSGVLEMRSLSRRLMAEQGKELGLIVIDYLQLMEGSTPDNRVQELSRITRGLKGMARELNVPVIALSQLSRGVESRT
NKRPMLSDLRESGSIEQDADLVLMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA
>Mature_472_residues
MVSAPLPNNGDTAAEGSRPFRKASSTSREPSFEALPDSVPPQNLEAEEAVLGGILLDPDAIGRVVDVLQPEAFYLNAHRE
IFRTALMLHSQGKPTDLTAMSAWLADTGSLEKVGGSNRLIELVERVASTASIEQVARLVMDKFLRRQLIRSGNEVIQLGF
DQGLPMEQVLDKAEQTIFAISQEKPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
PAMGKTSMVLNLTKNVAQLHNLPVCLFSLEMSKEQLTYRLLSMEVGIESSRLRTGRLRQEEWPLLGQGINTLGQLPIYID
DKPNSGVLEMRSLSRRLMAEQGKELGLIVIDYLQLMEGSTPDNRVQELSRITRGLKGMARELNVPVIALSQLSRGVESRT
NKRPMLSDLRESGSIEQDADLVLMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=464, Percent_Identity=42.8879310344828, Blast_Score=356, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52213; Mature: 52213

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAPLPNNGDTAAEGSRPFRKASSTSREPSFEALPDSVPPQNLEAEEAVLGGILLDPDA
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHCCEEECHHH
IGRVVDVLQPEAFYLNAHREIFRTALMLHSQGKPTDLTAMSAWLADTGSLEKVGGSNRLI
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCCHHHH
ELVERVASTASIEQVARLVMDKFLRRQLIRSGNEVIQLGFDQGLPMEQVLDKAEQTIFAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEEEE
SQEKPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCEEEEECC
PAMGKTSMVLNLTKNVAQLHNLPVCLFSLEMSKEQLTYRLLSMEVGIESSRLRTGRLRQE
CCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCC
EWPLLGQGINTLGQLPIYIDDKPNSGVLEMRSLSRRLMAEQGKELGLIVIDYLQLMEGST
CCCCHHHCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
PDNRVQELSRITRGLKGMARELNVPVIALSQLSRGVESRTNKRPMLSDLRESGSIEQDAD
CHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
LVLMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA
EEEEEEECCCCCCCCCCCCCHHHHEEECCCCCCEEEEEEECCCHHHHHHCCC
>Mature Secondary Structure
MVSAPLPNNGDTAAEGSRPFRKASSTSREPSFEALPDSVPPQNLEAEEAVLGGILLDPDA
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHCCEEECHHH
IGRVVDVLQPEAFYLNAHREIFRTALMLHSQGKPTDLTAMSAWLADTGSLEKVGGSNRLI
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCCHHHH
ELVERVASTASIEQVARLVMDKFLRRQLIRSGNEVIQLGFDQGLPMEQVLDKAEQTIFAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEEEE
SQEKPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCEEEEECC
PAMGKTSMVLNLTKNVAQLHNLPVCLFSLEMSKEQLTYRLLSMEVGIESSRLRTGRLRQE
CCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCC
EWPLLGQGINTLGQLPIYIDDKPNSGVLEMRSLSRRLMAEQGKELGLIVIDYLQLMEGST
CCCCHHHCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
PDNRVQELSRITRGLKGMARELNVPVIALSQLSRGVESRTNKRPMLSDLRESGSIEQDAD
CHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
LVLMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA
EEEEEEECCCCCCCCCCCCCHHHHEEECCCCCCEEEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]