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The map label for this gene is mnmG [H]

Identifier: 33864441

GI number: 33864441

Start: 2303752

End: 2305713

Strand: Direct

Name: mnmG [H]

Synonym: PMT2177

Alternate gene names: 33864441

Gene position: 2303752-2305713 (Clockwise)

Preceding gene: 33864440

Following gene: 33864442

Centisome position: 95.56

GC content: 56.68

Gene sequence:

>1962_bases
ATGCCCTTCAGTGCTCCCCCAACCGAACACTTCGACGTCATCATCGTCGGCGGTGGGCACGCCGGTTGTGAAGCCGCGAT
TACGGCAGCGCGCTTAGGCCTAAGTACAGCTTTATTCACCCTCAACCTCGATCGGATTGCCTGGCAACCGTGTAATCCCG
CAGTGGGAGGGCCAGCCAAGAGTCAACTCGTGCATGAAGTCGATGCCCTTGGGGGGGTCATCGGACGCCTGGCCGATGCG
ACGGCGCTGCAAAAACGTGTGCTGAATGCAAGCCGCGGCCCTGCGGTCTGGGCCTTACGCGCCCAGACCGACAAACGCCT
CTATTCACGCCAAATGCTGCAGTTGCTTCAACAAACAGCCAACCTCTCTCTAAGAGAAGCGATGGTGACTGGCCTCGAAG
TCAAAGGTGATCCATCGGGTGGCGGGGAGCACTGGGAGCCAGCTCAAGGACATGCAGCACAAATCACAGGCGTACGGACC
TATTTCGGCAGTATTTATCGGGCCCAAGCCGTCGTGCTAACCACCGGCACGTTCCTTGGTGGTCAGATCTGGGTTGGCAA
TCAATCCATGCCGGCAGGTCGTGCCGGCGAGCAAGCCGCAGAAGGGCTCACCGAAGCCCTCGAGTCACTTGGCTTCCAAA
CCAATCGCCTCAAGACAGGCACTCCGGCCAGAGTCGATCGACGCAGCATTGCTCTCGATCAACTGGAAGAGCAACCCAGC
GATGCCGCTGATCGCTTCTTCTCCTTCGACCCAACCGCCTGGGTTAGCGGTGAACAGATGAGCTGCCACATCACCCGCAC
CACCGCATCAACCCATCAACTGATCAAAGAGAACCTCGAACTAACGCCTATCTATGGAGGCTTTCTCGACAGCAAAGGGC
CGCGTTACTGCCCATCAATCGAAGACAAGATCGTCCGTTTTGCTGACAAAGACTCTCACCAGATTTTTCTCGAACCGGAA
GGTCGCGACACGCCAGAAATTTATGTGCAGGGATTCTCCACCGGACTACCTGAGCGGTTGCAACTCGACTTGCTACGCAC
CCTGCCAGGGCTTGAACAATGCGTCATGCTGCGGCCTGCCTATGCAGTCGATTACGACTACCTTCCCGCCACACAGCTCA
GTCCTTCATTGCAAACCAAACGCGTCAAAGGGCTTTTCACTGCTGGGCAACTGAATGGCACAACCGGCTATGAAGAAGCC
GCCGCCCAAGGGCTGGTTGCCGGTCTCAATGCAGCCCGTCTCGTCCAAGGCCAAGAGCAAGTGCAATTTCCGCGCGAGGG
CAGCTATATCGGCACAATGATTGACGATCTTGTCAGCAAGGATCTACACGAGCCGTACCGGGTGCTCACCAGTCGAAGCG
AATACCGCCTGATTTTGCGGGGCGACAACGCAGACCGACGCCTCACGCCTCTGGGCTATCAGCTAGGCCTGATTGATGCA
CGCCGTTGGCAGCTGTTTCAACGCAAACAAACCGCTCTAGAGGATGAAAAACAACGACTCGAAAAGCAGCGACTCAAAGC
TAGCGATCCAGCCGCACCGGCCCTCGAGGCCAAAACAGGCGCAACAATCAAAGGATCCATAACTCTGGCCGATCTGCTCC
GACGTCCTGGCGTGCGTAGTGCTGATCTGATCGAGCATGGCCTAGTCGATCCAGAGCTTGCCCTAGGCGTAAGAGAAGGA
GCAGAAATTGATATCAAGTACAGCGGTTACCTACAACGGCAGCAACAACAAATTGATCAATTGAAGCGGCAGAGCCAAAG
ACGCCTACCTGCAAATCTCGACTACGCCAACATCAGCACCCTCTCTAAGGAAGCCCGCGAAAAACTAACCGCTGTAGGGC
CACTCAATTTCGCTCAGGCCAGCCAGATCCCCGGTGTTAGCAAGGCAGATCTCACCGCCCTGTTGGTCTGGCTGGAACTG
CAGAAGCGTCGAACCCTCGCCGCCTCTGGTCACGATCGATAA

Upstream 100 bases:

>100_bases
ACTACTGTTCGAGCCTCAATTCACACGGTTCCGCAACCTGGCGGCCTAAAGCAGCAATACATTGATGATTTCTACTGTTC
TCTAGAACGTCACGGGCCCC

Downstream 100 bases:

>100_bases
CGTTGGATGTCATTGGAAACGAACGGTGCCCTCTCAGCTCACCACCTCGCGGGCCTACTGGAACCTACGAGCTGAAAAGG
TGATGGACCGCGTCTTCCAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MPFSAPPTEHFDVIIVGGGHAGCEAAITAARLGLSTALFTLNLDRIAWQPCNPAVGGPAKSQLVHEVDALGGVIGRLADA
TALQKRVLNASRGPAVWALRAQTDKRLYSRQMLQLLQQTANLSLREAMVTGLEVKGDPSGGGEHWEPAQGHAAQITGVRT
YFGSIYRAQAVVLTTGTFLGGQIWVGNQSMPAGRAGEQAAEGLTEALESLGFQTNRLKTGTPARVDRRSIALDQLEEQPS
DAADRFFSFDPTAWVSGEQMSCHITRTTASTHQLIKENLELTPIYGGFLDSKGPRYCPSIEDKIVRFADKDSHQIFLEPE
GRDTPEIYVQGFSTGLPERLQLDLLRTLPGLEQCVMLRPAYAVDYDYLPATQLSPSLQTKRVKGLFTAGQLNGTTGYEEA
AAQGLVAGLNAARLVQGQEQVQFPREGSYIGTMIDDLVSKDLHEPYRVLTSRSEYRLILRGDNADRRLTPLGYQLGLIDA
RRWQLFQRKQTALEDEKQRLEKQRLKASDPAAPALEAKTGATIKGSITLADLLRRPGVRSADLIEHGLVDPELALGVREG
AEIDIKYSGYLQRQQQQIDQLKRQSQRRLPANLDYANISTLSKEAREKLTAVGPLNFAQASQIPGVSKADLTALLVWLEL
QKRRTLAASGHDR

Sequences:

>Translated_653_residues
MPFSAPPTEHFDVIIVGGGHAGCEAAITAARLGLSTALFTLNLDRIAWQPCNPAVGGPAKSQLVHEVDALGGVIGRLADA
TALQKRVLNASRGPAVWALRAQTDKRLYSRQMLQLLQQTANLSLREAMVTGLEVKGDPSGGGEHWEPAQGHAAQITGVRT
YFGSIYRAQAVVLTTGTFLGGQIWVGNQSMPAGRAGEQAAEGLTEALESLGFQTNRLKTGTPARVDRRSIALDQLEEQPS
DAADRFFSFDPTAWVSGEQMSCHITRTTASTHQLIKENLELTPIYGGFLDSKGPRYCPSIEDKIVRFADKDSHQIFLEPE
GRDTPEIYVQGFSTGLPERLQLDLLRTLPGLEQCVMLRPAYAVDYDYLPATQLSPSLQTKRVKGLFTAGQLNGTTGYEEA
AAQGLVAGLNAARLVQGQEQVQFPREGSYIGTMIDDLVSKDLHEPYRVLTSRSEYRLILRGDNADRRLTPLGYQLGLIDA
RRWQLFQRKQTALEDEKQRLEKQRLKASDPAAPALEAKTGATIKGSITLADLLRRPGVRSADLIEHGLVDPELALGVREG
AEIDIKYSGYLQRQQQQIDQLKRQSQRRLPANLDYANISTLSKEAREKLTAVGPLNFAQASQIPGVSKADLTALLVWLEL
QKRRTLAASGHDR
>Mature_652_residues
PFSAPPTEHFDVIIVGGGHAGCEAAITAARLGLSTALFTLNLDRIAWQPCNPAVGGPAKSQLVHEVDALGGVIGRLADAT
ALQKRVLNASRGPAVWALRAQTDKRLYSRQMLQLLQQTANLSLREAMVTGLEVKGDPSGGGEHWEPAQGHAAQITGVRTY
FGSIYRAQAVVLTTGTFLGGQIWVGNQSMPAGRAGEQAAEGLTEALESLGFQTNRLKTGTPARVDRRSIALDQLEEQPSD
AADRFFSFDPTAWVSGEQMSCHITRTTASTHQLIKENLELTPIYGGFLDSKGPRYCPSIEDKIVRFADKDSHQIFLEPEG
RDTPEIYVQGFSTGLPERLQLDLLRTLPGLEQCVMLRPAYAVDYDYLPATQLSPSLQTKRVKGLFTAGQLNGTTGYEEAA
AQGLVAGLNAARLVQGQEQVQFPREGSYIGTMIDDLVSKDLHEPYRVLTSRSEYRLILRGDNADRRLTPLGYQLGLIDAR
RWQLFQRKQTALEDEKQRLEKQRLKASDPAAPALEAKTGATIKGSITLADLLRRPGVRSADLIEHGLVDPELALGVREGA
EIDIKYSGYLQRQQQQIDQLKRQSQRRLPANLDYANISTLSKEAREKLTAVGPLNFAQASQIPGVSKADLTALLVWLELQ
KRRTLAASGHDR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=655, Percent_Identity=40.4580152671756, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI19882217, Length=680, Percent_Identity=38.9705882352941, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI183227703, Length=695, Percent_Identity=38.1294964028777, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI2367273, Length=632, Percent_Identity=45.253164556962, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17534255, Length=633, Percent_Identity=38.7045813586098, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321202, Length=654, Percent_Identity=40.3669724770642, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24658174, Length=647, Percent_Identity=40.9582689335394, Blast_Score=429, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71501; Mature: 71370

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSAPPTEHFDVIIVGGGHAGCEAAITAARLGLSTALFTLNLDRIAWQPCNPAVGGPAK
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEHHHHCCCCCCCCCCCCHH
SQLVHEVDALGGVIGRLADATALQKRVLNASRGPAVWALRAQTDKRLYSRQMLQLLQQTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHH
NLSLREAMVTGLEVKGDPSGGGEHWEPAQGHAAQITGVRTYFGSIYRAQAVVLTTGTFLG
CCHHHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHEEEEEEECCEEC
GQIWVGNQSMPAGRAGEQAAEGLTEALESLGFQTNRLKTGTPARVDRRSIALDQLEEQPS
CEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
DAADRFFSFDPTAWVSGEQMSCHITRTTASTHQLIKENLELTPIYGGFLDSKGPRYCPSI
HHHHHHCCCCCCCEECCCCEEEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
EDKIVRFADKDSHQIFLEPEGRDTPEIYVQGFSTGLPERLQLDLLRTLPGLEQCVMLRPA
HHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCC
YAVDYDYLPATQLSPSLQTKRVKGLFTAGQLNGTTGYEEAAAQGLVAGLNAARLVQGQEQ
CEECCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCHHH
VQFPREGSYIGTMIDDLVSKDLHEPYRVLTSRSEYRLILRGDNADRRLTPLGYQLGLIDA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCEEEECCCH
RRWQLFQRKQTALEDEKQRLEKQRLKASDPAAPALEAKTGATIKGSITLADLLRRPGVRS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCEEHHHHHHCCCCCC
ADLIEHGLVDPELALGVREGAEIDIKYSGYLQRQQQQIDQLKRQSQRRLPANLDYANIST
HHHHHHCCCCHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LSKEAREKLTAVGPLNFAQASQIPGVSKADLTALLVWLELQKRRTLAASGHDR
HHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PFSAPPTEHFDVIIVGGGHAGCEAAITAARLGLSTALFTLNLDRIAWQPCNPAVGGPAK
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEHHHHCCCCCCCCCCCCHH
SQLVHEVDALGGVIGRLADATALQKRVLNASRGPAVWALRAQTDKRLYSRQMLQLLQQTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHH
NLSLREAMVTGLEVKGDPSGGGEHWEPAQGHAAQITGVRTYFGSIYRAQAVVLTTGTFLG
CCHHHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHEEEEEEECCEEC
GQIWVGNQSMPAGRAGEQAAEGLTEALESLGFQTNRLKTGTPARVDRRSIALDQLEEQPS
CEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
DAADRFFSFDPTAWVSGEQMSCHITRTTASTHQLIKENLELTPIYGGFLDSKGPRYCPSI
HHHHHHCCCCCCCEECCCCEEEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
EDKIVRFADKDSHQIFLEPEGRDTPEIYVQGFSTGLPERLQLDLLRTLPGLEQCVMLRPA
HHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCC
YAVDYDYLPATQLSPSLQTKRVKGLFTAGQLNGTTGYEEAAAQGLVAGLNAARLVQGQEQ
CEECCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCHHH
VQFPREGSYIGTMIDDLVSKDLHEPYRVLTSRSEYRLILRGDNADRRLTPLGYQLGLIDA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCEEEECCCH
RRWQLFQRKQTALEDEKQRLEKQRLKASDPAAPALEAKTGATIKGSITLADLLRRPGVRS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCEEHHHHHHCCCCCC
ADLIEHGLVDPELALGVREGAEIDIKYSGYLQRQQQQIDQLKRQSQRRLPANLDYANIST
HHHHHHCCCCHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LSKEAREKLTAVGPLNFAQASQIPGVSKADLTALLVWLELQKRRTLAASGHDR
HHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA