Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 33864403

GI number: 33864403

Start: 2250652

End: 2252139

Strand: Reverse

Name: gatB [H]

Synonym: PMT2139

Alternate gene names: 33864403

Gene position: 2252139-2250652 (Counterclockwise)

Preceding gene: 33864410

Following gene: 33864399

Centisome position: 93.42

GC content: 54.1

Gene sequence:

>1488_bases
ATGACATTGGCCACCAGCTCGGCTAACGGCGCATGGGAAGCCGTGATCGGTCTGGAGACCCACGTGCAGCTAGGCACCAA
TAGCAAAATCTTCACATGCGCTTCCACGACCTTTGGCGACGATCCCAACACCCACATCGACCCTGTTGTGTGTGGCCTGC
CTGGCACGTTGCCGGTGCTGAATCAGATGGTGTTGGAGTACGCGGTCAAGGCGGCGATGGCACTCAACCTCAACATTGCT
GAGCACAGCAAGTTTGATCGCAAACAGTATTTCTATCCCGATCTACCGAAGAACTATCAGATCTCTCAATTTGATGAGCC
GATTGCAGAAGAAGGTTGGATTGAGGTGGAAGTTGCGGAGAAGGGGAAGGACACTTATCTCAAGCGTATTGGCATCGAGC
GTCTCCATATGGAGGAGGATGCCGGCAAGCTCGTTCATGCTGGTAGCGATCGGCTCGCTGGCTCCACCCACTCCCTGGTG
GATTACAACCGGGCTGGCGTTGCGTTAGCCGAAATTGTTAGCAAGCCTGATCTGCGCACTGGGCGTGAGGCGGCGGAATA
TGCCTCGGAGATTCGCAGGATCATGCGTTATCTAGGGGTATCTGATGGAAACATGCAGGAAGGTTCTTTGCGTTGTGATG
TCAATATCTCCGTGCGCCAAGGAGCCGATGCTCCCTTTGGCACCAAGGTTGAGATCAAAAACATGAACTCGTTTTCGGCA
ATCCAGAAAGCTTGTGAGTACGAGATTCAGCGCCAGATCAAGGTCTATGAGGCCGGTGAAGCAGTGGTGCAGGAAACCCG
CCTTTGGGATGAGGGCAAGCAACTCACCAAGAGCATGCGTTCGAAGGAAGGCAGCAGCGATTACCGCTATTTTCCGGACC
CTGATCTAGGTCCGATTGAGGTGATGGCATCGGTACGTGAGGGTTGGCGAGATGAACTACCCGAGTTACCGGCAGCGAAG
CGTCATCGCTATGCCGAAGAGTTTGGTTTGTCGGTGTATGACGCCAGGGTGCTTACTGATGAGTATCCGATGGCGGAATA
CTTCGAGGCGGCAGTGGCGGCAGGTGCCGAGGCGAAGGGAGTGGCGAATTGGATCCAAGGCGATCTTGCTGCTTACGTGA
ATGCCAATCGTATTTCTTATTCCACGTTGCCCTTTCGACCGGAGCAGTTGGCAGAGATGGTGCAACTGATCGACGGCGGC
AAAATCAGCGGCAAGATCGCCAAGGAAATCTTGCCTGAGTTGCTGGAGAAGGGCGGCTCACCCGAGGCGATTGTTGATCA
GCGTGGGCTTGGGATGATCAGTGATCCGGCGGCTATCACAACGATTGTGGAGGAATTGTTGGCCGTTCATCCTCAGGAAG
TAGAGGCCTTCCGCGGCGGAAAGACCAAGCTGCAGAGTTTCTTTGTTGGTCAACTGATGAAAAAGACTGGCGGTAAAGCC
GATCCCAAGCTCGCCAATCAGATTCTCAGCAAGAAGTTGAAGGGTTGA

Upstream 100 bases:

>100_bases
TCCAGATCAGTTCAGTGTTTCAGCCTGTTGACCCAACTGCGGCCATGTGAGGGAACCTTTCCATAGGATCAAGAACATAT
TTAGTGATTGGAGATCGGCA

Downstream 100 bases:

>100_bases
TTCCCTTGGCAGCTTAATCAAAAGCTAGCAATAGTTCTTTTGTTAATGCGGTAGATCGAACTCATATAACTTGTTTGACT
GATGAGAAATATATGCTATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTLATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVLNQMVLEYAVKAAMALNLNIA
EHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAEKGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLV
DYNRAGVALAEIVSKPDLRTGREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
IQKACEYEIQRQIKVYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVMASVREGWRDELPELPAAK
RHRYAEEFGLSVYDARVLTDEYPMAEYFEAAVAAGAEAKGVANWIQGDLAAYVNANRISYSTLPFRPEQLAEMVQLIDGG
KISGKIAKEILPELLEKGGSPEAIVDQRGLGMISDPAAITTIVEELLAVHPQEVEAFRGGKTKLQSFFVGQLMKKTGGKA
DPKLANQILSKKLKG

Sequences:

>Translated_495_residues
MTLATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVLNQMVLEYAVKAAMALNLNIA
EHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAEKGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLV
DYNRAGVALAEIVSKPDLRTGREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
IQKACEYEIQRQIKVYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVMASVREGWRDELPELPAAK
RHRYAEEFGLSVYDARVLTDEYPMAEYFEAAVAAGAEAKGVANWIQGDLAAYVNANRISYSTLPFRPEQLAEMVQLIDGG
KISGKIAKEILPELLEKGGSPEAIVDQRGLGMISDPAAITTIVEELLAVHPQEVEAFRGGKTKLQSFFVGQLMKKTGGKA
DPKLANQILSKKLKG
>Mature_494_residues
TLATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVLNQMVLEYAVKAAMALNLNIAE
HSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAEKGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLVD
YNRAGVALAEIVSKPDLRTGREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSAI
QKACEYEIQRQIKVYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVMASVREGWRDELPELPAAKR
HRYAEEFGLSVYDARVLTDEYPMAEYFEAAVAAGAEAKGVANWIQGDLAAYVNANRISYSTLPFRPEQLAEMVQLIDGGK
ISGKIAKEILPELLEKGGSPEAIVDQRGLGMISDPAAITTIVEELLAVHPQEVEAFRGGKTKLQSFFVGQLMKKTGGKAD
PKLANQILSKKLKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=506, Percent_Identity=36.3636363636364, Blast_Score=315, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI115534094, Length=304, Percent_Identity=39.8026315789474, Blast_Score=211, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=503, Percent_Identity=30.8151093439364, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45550808, Length=500, Percent_Identity=34.4, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54501; Mature: 54370

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVL
CCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCHHHHH
NQMVLEYAVKAAMALNLNIAEHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAE
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECC
KGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLVDYNRAGVALAEIVSKPDLRT
CCHHHHHHHHCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCC
GREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHH
IQKACEYEIQRQIKVYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VMASVREGWRDELPELPAAKRHRYAEEFGLSVYDARVLTDEYPMAEYFEAAVAAGAEAKG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEHEEECCCCCHHHHHHHHHHCCCCHHH
VANWIQGDLAAYVNANRISYSTLPFRPEQLAEMVQLIDGGKISGKIAKEILPELLEKGGS
HHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PEAIVDQRGLGMISDPAAITTIVEELLAVHPQEVEAFRGGKTKLQSFFVGQLMKKTGGKA
CCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
DPKLANQILSKKLKG
CHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVL
CCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCHHHHH
NQMVLEYAVKAAMALNLNIAEHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAE
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECC
KGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLVDYNRAGVALAEIVSKPDLRT
CCHHHHHHHHCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCC
GREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHH
IQKACEYEIQRQIKVYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VMASVREGWRDELPELPAAKRHRYAEEFGLSVYDARVLTDEYPMAEYFEAAVAAGAEAKG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEHEEECCCCCHHHHHHHHHHCCCCHHH
VANWIQGDLAAYVNANRISYSTLPFRPEQLAEMVQLIDGGKISGKIAKEILPELLEKGGS
HHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PEAIVDQRGLGMISDPAAITTIVEELLAVHPQEVEAFRGGKTKLQSFFVGQLMKKTGGKA
CCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
DPKLANQILSKKLKG
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA