Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 33864347

GI number: 33864347

Start: 2196566

End: 2197861

Strand: Reverse

Name: eno [H]

Synonym: PMT2083

Alternate gene names: 33864347

Gene position: 2197861-2196566 (Counterclockwise)

Preceding gene: 33864356

Following gene: 33864340

Centisome position: 91.16

GC content: 54.48

Gene sequence:

>1296_bases
GTGATCGATTCCCTCGACCTCGTCATTGACACCATCGTGGCCAGAGAGGTTCTGGACTCTCGCGGCAACCCCACAGTTGA
AGCAGAAGTGTTGCTCGAAGCAGGTGCTATCGGTAGGGCCATCGTGCCCAGCGGAGCAAGCACCGGTGCCCATGAGGCGC
ATGAATTAAGAGATGGCGACAGCCGCTACCTGGGCAAAGGGGTGACTAAGGCTGTGAACCATATCGAGGATCGGATTGCC
CCAGCGCTCTGTGGGATCTCTTCCCTAGATCAGGCCAGTGTTGATGGCACCATGCAAGAGCTTGATGGCAGCGACAACAA
ATCAAGCCTGGGCGCAAACGCGATCCTGGCCGTGAGCATGGCCACTGCCCGCGCGGCCGCCAATGGCTTGGGTTTGCCCC
TCTACCGCTACCTGGGTGGCCCCATGGCCTCATTGCTGCCCGTACCGCTGATGAATGTGATCAACGGCGGTGCGCATGCT
GCTAACAATCTCGATTTTCAGGAATTCATGCTGGTGCCCCATGGAGCAAGCAGCTTCCGAGAATCGCTGCGCATGGGCGC
AGAAGTATTCCACACACTCAAGGGCCTGCTCAGTGCTCAGGGGCTCTCAACGGCAGTTGGAGATGAAGGCGGCTTTGCAC
CCAATCTGGCCAACAATGATGCAGCAGGAGATCTGCTCATTCAAGCAATCGAAAAAGCAGGCTATTCGCCAGGTAAGGAT
ATTTCCTTGGCACTAGATGTAGCCAGCACAGAGTTCTATCAAGACGGCTGCTATGCCTTCGGGGGTGGTCGTTACACCAG
TACCGAAATGGTCAATGAGCTCGAAAAACTTGTTGATCGCTACCCAATTATTTCGATCGAAGATGGACTAGCAGAAGATG
ATTGGCAAGGTTGGGCTCTACTCACAAAAAAATTGGGGAAGCGTATCCAACTGATAGGTGACGATATTTTTGTAACGAGT
ACTAAGCGCCTACAGCAGGGAATTGATCAAAACGTAGCCAATTCCATCCTGATTAAAGTGAATCAGATCGGCTCGCTAAC
GGAAACGCTTCAGGCCATTGATCTTGCTGGGCGCTCTGGTTACACCAGCGTAATCAGCCACCGCAGCGGCGAAACGGAAG
ACACCACAATTGCAGATCTTGCCGTTGCCACCCGTGCTGGGCAGATCAAAACGGGCTCCCTAAGTCGCAGTGAACGGGTA
GCGAAATACAACCAACTGCTGCGAATCGAAGACGAACTCGGTAGCCAAGCGGTCTATGCCGGTGCCACAGGACAAGGGCC
ACGGGGGCGAAGCTAA

Upstream 100 bases:

>100_bases
CTACGACTGACAACAGCCTCAGCATCAGTAAAAGGGCTAGGCGGTACACAGTCCGAGGGATGGCAAACCATAGGATCCAC
GCAACTTCAATTCGTCACAG

Downstream 100 bases:

>100_bases
GCAAAGGCCTCAAAGGGGCTGGACGGGGATCACTTTGGAAGACTTTCAGCGACTCTGACTCGACAGATGATCCATGCGCG
ACTCACGTCCCAGCTTGATC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGDSRYLGKGVTKAVNHIEDRIA
PALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSMATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHA
ANNLDFQEFMLVPHGASSFRESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLANNDAAGDLLIQAIEKAGYSPGKD
ISLALDVASTEFYQDGCYAFGGGRYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWALLTKKLGKRIQLIGDDIFVTS
TKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSGYTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERV
AKYNQLLRIEDELGSQAVYAGATGQGPRGRS

Sequences:

>Translated_431_residues
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGDSRYLGKGVTKAVNHIEDRIA
PALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSMATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHA
ANNLDFQEFMLVPHGASSFRESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLANNDAAGDLLIQAIEKAGYSPGKD
ISLALDVASTEFYQDGCYAFGGGRYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWALLTKKLGKRIQLIGDDIFVTS
TKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSGYTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERV
AKYNQLLRIEDELGSQAVYAGATGQGPRGRS
>Mature_431_residues
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGDSRYLGKGVTKAVNHIEDRIA
PALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSMATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHA
ANNLDFQEFMLVPHGASSFRESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLANNDAAGDLLIQAIEKAGYSPGKD
ISLALDVASTEFYQDGCYAFGGGRYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWALLTKKLGKRIQLIGDDIFVTS
TKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSGYTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERV
AKYNQLLRIEDELGSQAVYAGATGQGPRGRS

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=52.2935779816514, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=439, Percent_Identity=51.25284738041, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=439, Percent_Identity=51.25284738041, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=439, Percent_Identity=50.1138952164009, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=437, Percent_Identity=46.4530892448513, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=28.0597014925373, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=28.0597014925373, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=28.0597014925373, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI310129182, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310110045, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310120572, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789141, Length=421, Percent_Identity=60.8076009501188, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=53.4246575342466, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=53.4246575342466, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=196, Percent_Identity=48.9795918367347, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.6535796766744, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=48.0459770114943, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=48.0459770114943, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=48.0459770114943, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=48.9607390300231, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=438, Percent_Identity=48.8584474885845, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=438, Percent_Identity=48.8584474885845, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45403; Mature: 45403

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGD
CCCHHHHHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCCEECCCCCCCCCHHHHHHCCCC
SRYLGKGVTKAVNHIEDRIAPALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHHH
ATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHAANNLDFQEFMLVPHGASSFR
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHH
ESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLANNDAAGDLLIQAIEKAGYSPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
ISLALDVASTEFYQDGCYAFGGGRYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWAL
CEEEEEEHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTKKLGKRIQLIGDDIFVTSTKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSG
HHHHHCCCEEEECCCEEEECHHHHHHHHHHHHHHHEEEEEHHHCHHHHHHHHHHHCCCCC
YTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERVAKYNQLLRIEDELGSQAVYA
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
GATGQGPRGRS
CCCCCCCCCCC
>Mature Secondary Structure
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGD
CCCHHHHHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCCEECCCCCCCCCHHHHHHCCCC
SRYLGKGVTKAVNHIEDRIAPALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHHH
ATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHAANNLDFQEFMLVPHGASSFR
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHH
ESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLANNDAAGDLLIQAIEKAGYSPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
ISLALDVASTEFYQDGCYAFGGGRYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWAL
CEEEEEEHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTKKLGKRIQLIGDDIFVTSTKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSG
HHHHHCCCEEEECCCEEEECHHHHHHHHHHHHHHHEEEEEHHHCHHHHHHHHHHHCCCCC
YTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERVAKYNQLLRIEDELGSQAVYA
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
GATGQGPRGRS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA