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The map label for this gene is trpB

Identifier: 33864292

GI number: 33864292

Start: 2138965

End: 2140275

Strand: Reverse

Name: trpB

Synonym: PMT2027

Alternate gene names: 33864292

Gene position: 2140275-2138965 (Counterclockwise)

Preceding gene: 33864295

Following gene: 33864290

Centisome position: 88.78

GC content: 56.83

Gene sequence:

>1311_bases
TTGCTGCTTGGGGTGATTCTTTCGATTCCTAGGCTGGTGTTGTTCTTGCCTGGCGTACCGGTGACCAGCACTCTGCCCTC
TCAGCCCAAAGATGCGGACCTCGCTGTGAGTGCGCGACCTTCTGCCCAGGGGCGTTTCGGCAGATATGGGGGCCAGTACG
TGCCCGAAACCTTGATGCCTGCCTTGGCTGAACTGGAGCAAGCAGCTGCGCTGGCATGGAAAGATCCTGCCTTCACTGCT
GAGTTGGATCGGTTGCTGCGTAGCTATGTGGGTCGGGCGACGCCGCTTTATGAGGCTGAGCGCCTCACAGCTCATTACTG
CCGTTCAGACGGAGGACCCAGGATCTGGCTTAAGCGTGAGGATCTCAATCACACAGGCGCCCACAAAATCAATAACGCTT
TGGGTCAGGCTCTGTTGGCGTTACGCATGGGTAAAAAGCGGATCATTGCCGAAACAGGTGCGGGTCAACACGGTGTGGCC
ACGGCCACGGTGTGTGCCCGTTTTGGTCTTGAGTGTGTCGTCTACATGGGGGCTGAGGACATGCGCCGTCAGGCGTTGAA
TGTGTTCCGCATGCGTCTGCTGGGAGCCACAGTGCAGCCTGTCACCGCAGGCACGGCCACGCTGAAGGACGCGACGAGTG
AGGCGATTCGTGATTGGGTCACCAATGTGGAAACCACCCATTACATCCTTGGTTCGGTGGCTGGTCCCCATCCCTATCCG
ATGCTTGTTCGCGACTTTCATGCCGTGATCGGTCAGGAGGCCAGGCAGCAGTGTGCTGAGGCTTTCGGTCGCTTGCCTGA
TGTTCTTTTGGCGTGCGTTGGTGGTGGGTCCAATGCCATGGGTTTGTTTCATCCCTTCGTGCAGGACCGCTCTGTGCGCA
TGATTGGAGTGGAAGCTGCTGGTGATGGTGTTGAAACTGGTCGTCATGCGGCAACAATCACCGAAGGTCGAGTAGGCGTC
TTGCATGGAGCGATGAGTTTGTTGCTTCAGGATGATGAAGGTCAGGTGCAAGAGGCGCATTCGATTAGTGCCGGCCTCGA
CTACCCAGGAGTGGGTCCAGAACACAGCTACCTGTGTGAGATTGGTCGGGCGGAGTATGCCGCGGTTAGCGATCAGCAGG
CTTTGGATGCATTGCGCTTGGTGAGTGAACTAGAAGGAATTATTCCTGCATTAGAGACGGCCCATGCCTTTGCTTGGTTA
GAGACGCTTTGCCCCACTCTTGCCGCTGGAACAGAAGTGGTAATCAATTGCTCTGGCCGCGGCGATAAGGACGTCAACAC
CGTGGCAGAGAAACTGGGCAATCAGCTTTGA

Upstream 100 bases:

>100_bases
TTTCGTTGTTGGTCTGGCTTGGGCAGGGCCCATGGGGCGGATGGTTGAGTTGGCTGGCAAAGACTCCCTCTAACTTTGCG
GCTTCAAACGAAAGCACTCT

Downstream 100 bases:

>100_bases
ATGAAAACTGCAGCCATGCGGTAATAGGCGTTTTGCTTGAGCCATGGCCTTGCGGGATCAGAGCTCGATCATTAGCAAGG
GGTTAACGAGTAACTGATCA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLLGVILSIPRLVLFLPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKDPAFTA
ELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVA
TATVCARFGLECVVYMGAEDMRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYP
MLVRDFHAVIGQEARQQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQDRSVRMIGVEAAGDGVETGRHAATITEGRVGV
LHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWL
ETLCPTLAAGTEVVINCSGRGDKDVNTVAEKLGNQL

Sequences:

>Translated_436_residues
MLLGVILSIPRLVLFLPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKDPAFTA
ELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVA
TATVCARFGLECVVYMGAEDMRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYP
MLVRDFHAVIGQEARQQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQDRSVRMIGVEAAGDGVETGRHAATITEGRVGV
LHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWL
ETLCPTLAAGTEVVINCSGRGDKDVNTVAEKLGNQL
>Mature_436_residues
MLLGVILSIPRLVLFLPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKDPAFTA
ELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVA
TATVCARFGLECVVYMGAEDMRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYP
MLVRDFHAVIGQEARQQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQDRSVRMIGVEAAGDGVETGRHAATITEGRVGV
LHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWL
ETLCPTLAAGTEVVINCSGRGDKDVNTVAEKLGNQL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=388, Percent_Identity=53.3505154639175, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6321412, Length=396, Percent_Identity=60.3535353535353, Blast_Score=480, Evalue=1e-136,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_PROMM (Q7TUL2)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895852.1
- ProteinModelPortal:   Q7TUL2
- SMR:   Q7TUL2
- STRING:   Q7TUL2
- GeneID:   1729339
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT2027
- NMPDR:   fig|74547.1.peg.2019
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   PMAR74547:PMT2027-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 46619; Mature: 46619

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGVILSIPRLVLFLPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMP
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHH
ALAELEQAAALAWKDPAFTAELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKRE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
DLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVYMGAED
CCCCCCHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHCCCEEEEECHHH
MRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYP
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCH
MLVRDFHAVIGQEARQQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQDRSVRMIGVEAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEEC
GDGVETGRHAATITEGRVGVLHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCE
CCCCCCCCCCEEECCCCHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
IGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVINCSGR
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GDKDVNTVAEKLGNQL
CCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLLGVILSIPRLVLFLPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMP
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHH
ALAELEQAAALAWKDPAFTAELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKRE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
DLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVYMGAED
CCCCCCHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHCCCEEEEECHHH
MRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYP
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCH
MLVRDFHAVIGQEARQQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQDRSVRMIGVEAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEEC
GDGVETGRHAATITEGRVGVLHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCE
CCCCCCCCCCEEECCCCHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
IGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVINCSGR
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GDKDVNTVAEKLGNQL
CCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642