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The map label for this gene is cysK2 [H]

Identifier: 33864243

GI number: 33864243

Start: 2087928

End: 2088896

Strand: Reverse

Name: cysK2 [H]

Synonym: PMT1978

Alternate gene names: 33864243

Gene position: 2088896-2087928 (Counterclockwise)

Preceding gene: 33864244

Following gene: 33864242

Centisome position: 86.64

GC content: 58.2

Gene sequence:

>969_bases
ATGCCCATTGCTGCGGACATCACGGCCCTGGTTGGCCGAACCCCAATAGTGCGGCTGAATCGCTTGCCACAGTTGAGTGG
TTGCCATGCGGAGGTGCTGGCCAAGTTGGAAAGCTTCAACCCCACTGCCTCGGTTAAAGACCGCATCGCGGGGGCCATGA
TTCAAGCAGCGGAGGAAGCTGGAACCATTGCTCCGGGGCGCACCGTGTTGGTGGAACCCACCAGTGGCAACACTGGGATC
GCCTTGGCCATGGTGGCTGCCGCCCGAGGCTATCGCCTCATTCTCACCATGCCGGACACGATGAGTACAGAGCGTCGCGC
CATGCTTAGGGCCTATGGCGCTGAACTGCAACTCACCCCAGGCAGCGATGGAATGACTGGTTCCATTGAGTTGGCAAAGG
AGTTGGTGGCAACCATCCCTGAGGCTTATCTGCTGCAGCAGTTTGATAATCCTGCGAATCCGCAGGTGCATGAGCGTACA
ACCGCAGAGGAGATCTGGAATGATTGCGAGGGGCGGTTGGATGGTCTGATCACTGGAGTAGGAACGGGGGGCACACTCAC
AGGTTGTGCGCGTTTGCTCAAGCAACGCAATCCAAGCCTGCGAGTGTTTGCGGTAGAGCCAGCGTTAAGCCCTGTTTTGG
CAGGCGGGAGCCCTGCTCCCCATCGCATTCAGGGGATTGGTGCTGGCTTCATTCCTAGCGTTCTTGATCTCTCTTTGATC
GATGAGATCCTGCCGGTCTCTGATGACGACGCGATGGAGATGGGTCGGCGCTTGGCGAGGGAGGAGGGCTTGCTAAGTGG
GGTAAGCAGTGGCGCTGCTGTTGCGGCTGCACTGCAAGTGGGTAGTAGGGCTGAGATGGTGGGCAAGCGTCTTGTTGTTG
TCTTGGCAAGTTTTGGTGAGCGTTATCTATCAACGCCGATGTTTAGCGCTGCTTCGGCGGTGCCGGCGCAGCGTGATGGA
TACCTTTGA

Upstream 100 bases:

>100_bases
CTCGATCAATTTCAGCCCTCCGGAAACGGGGGGCTTTTTCATGGCTTGATCCCCATCGCTTTGTTTGGATGGGTCCGTGG
CTCTCCCTTCACTATCGTTT

Downstream 100 bases:

>100_bases
TGTCTTCAAAGCCGCTGGCTGCTATGGCTGCAGATCCTTACCAGGTGTTGGGAGTAACCTCCACCGCTAGCTCGGCTGAG
ATCAAGGCTGCCTATCGCCA

Product: O-acetylserine (thiol)-lyase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPIAADITALVGRTPIVRLNRLPQLSGCHAEVLAKLESFNPTASVKDRIAGAMIQAAEEAGTIAPGRTVLVEPTSGNTGI
ALAMVAAARGYRLILTMPDTMSTERRAMLRAYGAELQLTPGSDGMTGSIELAKELVATIPEAYLLQQFDNPANPQVHERT
TAEEIWNDCEGRLDGLITGVGTGGTLTGCARLLKQRNPSLRVFAVEPALSPVLAGGSPAPHRIQGIGAGFIPSVLDLSLI
DEILPVSDDDAMEMGRRLAREEGLLSGVSSGAAVAAALQVGSRAEMVGKRLVVVLASFGERYLSTPMFSAASAVPAQRDG
YL

Sequences:

>Translated_322_residues
MPIAADITALVGRTPIVRLNRLPQLSGCHAEVLAKLESFNPTASVKDRIAGAMIQAAEEAGTIAPGRTVLVEPTSGNTGI
ALAMVAAARGYRLILTMPDTMSTERRAMLRAYGAELQLTPGSDGMTGSIELAKELVATIPEAYLLQQFDNPANPQVHERT
TAEEIWNDCEGRLDGLITGVGTGGTLTGCARLLKQRNPSLRVFAVEPALSPVLAGGSPAPHRIQGIGAGFIPSVLDLSLI
DEILPVSDDDAMEMGRRLAREEGLLSGVSSGAAVAAALQVGSRAEMVGKRLVVVLASFGERYLSTPMFSAASAVPAQRDG
YL
>Mature_321_residues
PIAADITALVGRTPIVRLNRLPQLSGCHAEVLAKLESFNPTASVKDRIAGAMIQAAEEAGTIAPGRTVLVEPTSGNTGIA
LAMVAAARGYRLILTMPDTMSTERRAMLRAYGAELQLTPGSDGMTGSIELAKELVATIPEAYLLQQFDNPANPQVHERTT
AEEIWNDCEGRLDGLITGVGTGGTLTGCARLLKQRNPSLRVFAVEPALSPVLAGGSPAPHRIQGIGAGFIPSVLDLSLID
EILPVSDDDAMEMGRRLAREEGLLSGVSSGAAVAAALQVGSRAEMVGKRLVVVLASFGERYLSTPMFSAASAVPAQRDGY
L

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=42.5806451612903, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=42.5806451612903, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=42.5806451612903, Blast_Score=240, Evalue=1e-63,
Organism=Escherichia coli, GI1788754, Length=316, Percent_Identity=54.4303797468354, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=44.1471571906354, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=52.3333333333333, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17562970, Length=299, Percent_Identity=52.1739130434783, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=53.0944625407166, Blast_Score=312, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=52.901023890785, Blast_Score=307, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=50.1672240802676, Blast_Score=276, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=51.6431924882629, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17534315, Length=306, Percent_Identity=40.5228758169935, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=310, Percent_Identity=38.7096774193548, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=47.8991596638655, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321594, Length=287, Percent_Identity=39.0243902439024, Blast_Score=212, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=33.0275229357798, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643623, Length=313, Percent_Identity=39.9361022364217, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI20129101, Length=313, Percent_Identity=39.9361022364217, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33725; Mature: 33594

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIAADITALVGRTPIVRLNRLPQLSGCHAEVLAKLESFNPTASVKDRIAGAMIQAAEEA
CCCHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GTIAPGRTVLVEPTSGNTGIALAMVAAARGYRLILTMPDTMSTERRAMLRAYGAELQLTP
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEECC
GSDGMTGSIELAKELVATIPEAYLLQQFDNPANPQVHERTTAEEIWNDCEGRLDGLITGV
CCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEC
GTGGTLTGCARLLKQRNPSLRVFAVEPALSPVLAGGSPAPHRIQGIGAGFIPSVLDLSLI
CCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHH
DEILPVSDDDAMEMGRRLAREEGLLSGVSSGAAVAAALQVGSRAEMVGKRLVVVLASFGE
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RYLSTPMFSAASAVPAQRDGYL
HHHCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PIAADITALVGRTPIVRLNRLPQLSGCHAEVLAKLESFNPTASVKDRIAGAMIQAAEEA
CCHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GTIAPGRTVLVEPTSGNTGIALAMVAAARGYRLILTMPDTMSTERRAMLRAYGAELQLTP
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEECC
GSDGMTGSIELAKELVATIPEAYLLQQFDNPANPQVHERTTAEEIWNDCEGRLDGLITGV
CCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEC
GTGGTLTGCARLLKQRNPSLRVFAVEPALSPVLAGGSPAPHRIQGIGAGFIPSVLDLSLI
CCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHH
DEILPVSDDDAMEMGRRLAREEGLLSGVSSGAAVAAALQVGSRAEMVGKRLVVVLASFGE
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RYLSTPMFSAASAVPAQRDGYL
HHHCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]