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The map label for this gene is kdsA

Identifier: 33864191

GI number: 33864191

Start: 2033006

End: 2033857

Strand: Direct

Name: kdsA

Synonym: PMT1925

Alternate gene names: 33864191

Gene position: 2033006-2033857 (Clockwise)

Preceding gene: 33864185

Following gene: 33864198

Centisome position: 84.33

GC content: 55.99

Gene sequence:

>852_bases
ATGGCTGCCCGCAGCATCGCCCTCGGCACCATTCGTTTTGCCAACGATGCACCGTTCGTGCTGATTGGAGGCATCAATGT
GCTCGAGTCGCGGGAATTTGCACTTGAGGTTGCTGGCCATTACAAAACGGTTTGCACCAAGCTCGGAATTCCCCTGGTGT
TTAAGGCTTCCTTCGACAAGGCCAACCGCTCCTCCATTCACTCCTACCGAGGGCCTGGCCTCAGGGAAGGGCTCGAGATT
CTTCAAGTGGTGAAAGACACCCACGGGATCCCCGTGATTACCGATGTGCACAGCCCGGAAGAAGCAACCCCAGCGGCAGA
AGTCTGCGACATCATCCAGTTGCCCGCCTTCCTCGCGAGACAAACCGATCTCATTGAAGCAATGGCCAAAACTGGCGCAG
TCATCAACATCAAAAAGCCGCAGTTTCTCAGCCCATCGCAAATGTCAAACGTGGTGGAAAAGTTCCGCGAATGCGGCAAC
GAGAATTTGCTTATCTGCGAGCGGGGAAGCAACTTCGGCTATGACAATCTTGTGGTTGACATGCTCGCCTTCGGAGTAAT
GAAGCACTGCTGCAATGACCTACCCCTGATTTTTGATGTGACCCACGCGCTCCAGTGTCGCGACCCAAGTGGAGCTGCCT
CCGGTGGCCGCCGCAGCCAGGTGGTCGATCTAGCACGTTCTGGCATGGCAGTAGGCCTGGCAGGACTTTTCCTAGAATCT
CACCCAGACCCCGACAAGGCGCGCTGCGATGGTCCAAGTGCGTTGCCACTGGCGCTGCTTGAACCTTTTCTCCAGCAGGT
CAAAGCGATCGATGAGGTTGTGAAAGCACTCCCAACTCTCTCGGTGAGCTAG

Upstream 100 bases:

>100_bases
GGGATTGGAGCCGTCAACATCCGCCTTCATCACGTGATGGATTGAGATTAAGGCTGCTACTGCAAGAGCCCTAGGCTCCT
AAACATCAAAGCTTTAAGCC

Downstream 100 bases:

>100_bases
TTAAACAAACTGAAGATCAACTCTAAGTTGATTGCTGCCTACTGCTGACTGGAAGTCACCCATTGAGCATTCAAGGCAGC
CACCCCAGGTGCCAAGACCA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAARSIALGTIRFANDAPFVLIGGINVLESREFALEVAGHYKTVCTKLGIPLVFKASFDKANRSSIHSYRGPGLREGLEI
LQVVKDTHGIPVITDVHSPEEATPAAEVCDIIQLPAFLARQTDLIEAMAKTGAVINIKKPQFLSPSQMSNVVEKFRECGN
ENLLICERGSNFGYDNLVVDMLAFGVMKHCCNDLPLIFDVTHALQCRDPSGAASGGRRSQVVDLARSGMAVGLAGLFLES
HPDPDKARCDGPSALPLALLEPFLQQVKAIDEVVKALPTLSVS

Sequences:

>Translated_283_residues
MAARSIALGTIRFANDAPFVLIGGINVLESREFALEVAGHYKTVCTKLGIPLVFKASFDKANRSSIHSYRGPGLREGLEI
LQVVKDTHGIPVITDVHSPEEATPAAEVCDIIQLPAFLARQTDLIEAMAKTGAVINIKKPQFLSPSQMSNVVEKFRECGN
ENLLICERGSNFGYDNLVVDMLAFGVMKHCCNDLPLIFDVTHALQCRDPSGAASGGRRSQVVDLARSGMAVGLAGLFLES
HPDPDKARCDGPSALPLALLEPFLQQVKAIDEVVKALPTLSVS
>Mature_282_residues
AARSIALGTIRFANDAPFVLIGGINVLESREFALEVAGHYKTVCTKLGIPLVFKASFDKANRSSIHSYRGPGLREGLEIL
QVVKDTHGIPVITDVHSPEEATPAAEVCDIIQLPAFLARQTDLIEAMAKTGAVINIKKPQFLSPSQMSNVVEKFRECGNE
NLLICERGSNFGYDNLVVDMLAFGVMKHCCNDLPLIFDVTHALQCRDPSGAASGGRRSQVVDLARSGMAVGLAGLFLESH
PDPDKARCDGPSALPLALLEPFLQQVKAIDEVVKALPTLSVS

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=283, Percent_Identity=68.5512367491166, Blast_Score=423, Evalue=1e-120,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_PROMM (Q7V4M4)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895751.1
- ProteinModelPortal:   Q7V4M4
- SMR:   Q7V4M4
- STRING:   Q7V4M4
- GeneID:   1729196
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1925
- NMPDR:   fig|74547.1.peg.1918
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   QLILCDR
- ProtClustDB:   PRK05198
- BioCyc:   PMAR74547:PMT1925-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30435; Mature: 30303

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARSIALGTIRFANDAPFVLIGGINVLESREFALEVAGHYKTVCTKLGIPLVFKASFDK
CCCCCEEEEEEEECCCCCEEEECCCHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSIHSYRGPGLREGLEILQVVKDTHGIPVITDVHSPEEATPAAEVCDIIQLPAFLAR
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
QTDLIEAMAKTGAVINIKKPQFLSPSQMSNVVEKFRECGNENLLICERGSNFGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
MLAFGVMKHCCNDLPLIFDVTHALQCRDPSGAASGGRRSQVVDLARSGMAVGLAGLFLES
HHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHC
HPDPDKARCDGPSALPLALLEPFLQQVKAIDEVVKALPTLSVS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AARSIALGTIRFANDAPFVLIGGINVLESREFALEVAGHYKTVCTKLGIPLVFKASFDK
CCCCEEEEEEEECCCCCEEEECCCHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSIHSYRGPGLREGLEILQVVKDTHGIPVITDVHSPEEATPAAEVCDIIQLPAFLAR
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
QTDLIEAMAKTGAVINIKKPQFLSPSQMSNVVEKFRECGNENLLICERGSNFGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
MLAFGVMKHCCNDLPLIFDVTHALQCRDPSGAASGGRRSQVVDLARSGMAVGLAGLFLES
HHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHC
HPDPDKARCDGPSALPLALLEPFLQQVKAIDEVVKALPTLSVS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642