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The map label for this gene is tgt [H]

Identifier: 33864129

GI number: 33864129

Start: 1969507

End: 1970625

Strand: Direct

Name: tgt [H]

Synonym: PMT1862

Alternate gene names: 33864129

Gene position: 1969507-1970625 (Clockwise)

Preceding gene: 33864127

Following gene: 33864130

Centisome position: 81.69

GC content: 55.41

Gene sequence:

>1119_bases
GTGTTTGATTTTCAGATCAACGCCCACTGTTCACACACACGTGCTCGCGTTGGCTGCTTCCGCACTCCTCATGGTTCTGT
CAACACACCTCGTTTCATGCCTGTGGGCACGTTGGCAACGGTGAAAGGCATCACAGCCACTCAACTGGCCGACACCGGAG
CTCAGATGGTGTTAGCCAACACCTATCACCTCCATCTTCAGCCAGGCGAGGGGATCGTGGCCGATGCCGGTGGCCTGCAT
CGCTTCATGGGCTGGGACCGCCCACTGCTTACCGACTCAGGTGGCTTTCAGATTTTCAGCCTGGCAGACCTGAATCGTAT
TGATGATCACGGGGTCGTTTTTCGCAACCCTCGTAATGGCAGCCAGATTGAACTCACGCCAGAACGAGCCATCGAAATTC
AAATGGCCCTTGGTGCTGATGTCGCTATGGCCTTCGACCAATGCCCCCCATACCCAGCCAGCGAAAGCGATGTAGAGACT
GCCTGTAAGCGCACCCATGCATGGCTGGAACGCTGCTCGAACACCCATCAACACGCAAATCAAGCCCTATTCGGCATTGT
TCAGGGAGGGTGTTTTCCCCACCTCAGAGAGCAAAGTGCTCAAATCGTGGCCAGCTTTGGTTTGCCTGGCATCGCCATCG
GTGGCGTCAGCGTGGGTGAACCGGTTGAAGACATTCATCGCATCGTACGCCAGGTTTCTCCTCTGCTTCCCCAGGATCGC
CCCCGCTATCTGATGGGGATTGGCACCCTGCGAGAAATAGCGATTGCAGTTGCCAGCGGTATCGATCTATTCGACTGTGT
GCTGCCTACCCGCCTGGGAAGACATGGCACAGCACTTGTCGCTGGCGAGCGCTGGAATTTACGCAATGCCCGCTTTCGTG
AAGATCACACACCCCTAGATCAAAGCTGTACCTGTACAGCCTGCCGCCATCACAGCCGGGCTTATCTGCATCACTTGATT
CGCAACGAGGAGCTCCTAGGGCTCACGCTTCTAAGCCTGCACAACCTCACCCAGCTGATCAGATTCACCTCAGCTATTAG
CCAGGCAATTCAAGATGACTGTTTTTCAGAGGATTTCGCTCCTTGGCAACCGGACTCAGCAGCACATCACACGTGGTAA

Upstream 100 bases:

>100_bases
GCGCGCAAGATCTCCAACCTCCAGCCAAAGGCAAAGCCCAACGTAGGGTCATGATCGGCCTGGTGCCCTGTGGCGCTGAA
GTATCGCCGTAGCACTAGCC

Downstream 100 bases:

>100_bases
CGTCCATGAAACGCAGCTGAGCAACTAGGGATGGCAGCCTTCACTCTCGATCTGATGGCCCAACTGCCAGAGGCCTATCA
GGCCTACGCTCCAGCAGTGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFDFQINAHCSHTRARVGCFRTPHGSVNTPRFMPVGTLATVKGITATQLADTGAQMVLANTYHLHLQPGEGIVADAGGLH
RFMGWDRPLLTDSGGFQIFSLADLNRIDDHGVVFRNPRNGSQIELTPERAIEIQMALGADVAMAFDQCPPYPASESDVET
ACKRTHAWLERCSNTHQHANQALFGIVQGGCFPHLREQSAQIVASFGLPGIAIGGVSVGEPVEDIHRIVRQVSPLLPQDR
PRYLMGIGTLREIAIAVASGIDLFDCVLPTRLGRHGTALVAGERWNLRNARFREDHTPLDQSCTCTACRHHSRAYLHHLI
RNEELLGLTLLSLHNLTQLIRFTSAISQAIQDDCFSEDFAPWQPDSAAHHTW

Sequences:

>Translated_372_residues
MFDFQINAHCSHTRARVGCFRTPHGSVNTPRFMPVGTLATVKGITATQLADTGAQMVLANTYHLHLQPGEGIVADAGGLH
RFMGWDRPLLTDSGGFQIFSLADLNRIDDHGVVFRNPRNGSQIELTPERAIEIQMALGADVAMAFDQCPPYPASESDVET
ACKRTHAWLERCSNTHQHANQALFGIVQGGCFPHLREQSAQIVASFGLPGIAIGGVSVGEPVEDIHRIVRQVSPLLPQDR
PRYLMGIGTLREIAIAVASGIDLFDCVLPTRLGRHGTALVAGERWNLRNARFREDHTPLDQSCTCTACRHHSRAYLHHLI
RNEELLGLTLLSLHNLTQLIRFTSAISQAIQDDCFSEDFAPWQPDSAAHHTW
>Mature_372_residues
MFDFQINAHCSHTRARVGCFRTPHGSVNTPRFMPVGTLATVKGITATQLADTGAQMVLANTYHLHLQPGEGIVADAGGLH
RFMGWDRPLLTDSGGFQIFSLADLNRIDDHGVVFRNPRNGSQIELTPERAIEIQMALGADVAMAFDQCPPYPASESDVET
ACKRTHAWLERCSNTHQHANQALFGIVQGGCFPHLREQSAQIVASFGLPGIAIGGVSVGEPVEDIHRIVRQVSPLLPQDR
PRYLMGIGTLREIAIAVASGIDLFDCVLPTRLGRHGTALVAGERWNLRNARFREDHTPLDQSCTCTACRHHSRAYLHHLI
RNEELLGLTLLSLHNLTQLIRFTSAISQAIQDDCFSEDFAPWQPDSAAHHTW

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=43.3802816901408, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786607, Length=355, Percent_Identity=45.6338028169014, Blast_Score=327, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17544680, Length=344, Percent_Identity=42.1511627906977, Blast_Score=280, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=227, Percent_Identity=24.2290748898678, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=45.0704225352113, Blast_Score=321, Evalue=4e-88,
Organism=Drosophila melanogaster, GI21355311, Length=259, Percent_Identity=26.2548262548263, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41008; Mature: 41008

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFQINAHCSHTRARVGCFRTPHGSVNTPRFMPVGTLATVKGITATQLADTGAQMVLAN
CCEEEECCCCCCCHHHHCEEECCCCCCCCCCEEECCHHHHHCCCHHHHHHHCCCEEEEEE
TYHLHLQPGEGIVADAGGLHRFMGWDRPLLTDSGGFQIFSLADLNRIDDHGVVFRNPRNG
EEEEEEECCCCEEECCCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCCCCEEEECCCCC
SQIELTPERAIEIQMALGADVAMAFDQCPPYPASESDVETACKRTHAWLERCSNTHQHAN
CEEEECHHHEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
QALFGIVQGGCFPHLREQSAQIVASFGLPGIAIGGVSVGEPVEDIHRIVRQVSPLLPQDR
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCC
PRYLMGIGTLREIAIAVASGIDLFDCVLPTRLGRHGTALVAGERWNLRNARFREDHTPLD
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
QSCTCTACRHHSRAYLHHLIRNEELLGLTLLSLHNLTQLIRFTSAISQAIQDDCFSEDFA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWQPDSAAHHTW
CCCCCCCCCCCC
>Mature Secondary Structure
MFDFQINAHCSHTRARVGCFRTPHGSVNTPRFMPVGTLATVKGITATQLADTGAQMVLAN
CCEEEECCCCCCCHHHHCEEECCCCCCCCCCEEECCHHHHHCCCHHHHHHHCCCEEEEEE
TYHLHLQPGEGIVADAGGLHRFMGWDRPLLTDSGGFQIFSLADLNRIDDHGVVFRNPRNG
EEEEEEECCCCEEECCCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCCCCEEEECCCCC
SQIELTPERAIEIQMALGADVAMAFDQCPPYPASESDVETACKRTHAWLERCSNTHQHAN
CEEEECHHHEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
QALFGIVQGGCFPHLREQSAQIVASFGLPGIAIGGVSVGEPVEDIHRIVRQVSPLLPQDR
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCC
PRYLMGIGTLREIAIAVASGIDLFDCVLPTRLGRHGTALVAGERWNLRNARFREDHTPLD
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
QSCTCTACRHHSRAYLHHLIRNEELLGLTLLSLHNLTQLIRFTSAISQAIQDDCFSEDFA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWQPDSAAHHTW
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA