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The map label for this gene is amt1 [C]

Identifier: 33864120

GI number: 33864120

Start: 1960347

End: 1961825

Strand: Direct

Name: amt1 [C]

Synonym: PMT1853

Alternate gene names: 33864120

Gene position: 1960347-1961825 (Clockwise)

Preceding gene: 33864119

Following gene: 33864121

Centisome position: 81.31

GC content: 56.73

Gene sequence:

>1479_bases
ATGACAACTGCTCCGCTTCCGCGCTCCAGGCGGCGCACGGTACGCCTCCAGGAGGCCAGCCTCCTGGAGGGGCCAATGCT
CCTCCTGCGCAGCATCCGTGGCTTCAGCTCCAATCGCTCACTGACGTGGCTGGCTTGCGTGCCCTTGGCTCTCTTCGGCC
TAGGACTCTTCAACCTCTCGGCCCATGCCGCCGAGATGCCAGAACTGAATGCTGCCTTCCTAGCCAACAATCTCTGGCTT
CTGGTTGCAACGATTCTGGTGATCTTCATGAATGCCGGCTTCGCGATGGTCGAAGCAGGAATGTGTCGTCAAAAAAACGC
GGTCAACATTCTCGCCAAAAACCTATTTGTCTTTGCCCTAGCGGTATCGTCGTACTGGTTCATCGGCTATTCGCTGATGT
ACGGCGATCCTGTTTCGGCAGGATGGCTCTATTTCAACGGCCTGTTCTTTGATCCTGCTGTAACCCCTGAACTGATCAGC
GAAGCAGGTCTTGTCCCAAGCGTCGACTTCCTCTTCCAAGCAGCCTTTGCAGGCACAGCTGCAACTATCGTCTCAGGACT
CGTGGCAGAGCGCGTCAAGTTCGGCGAGTTCGTGGTGTTCTCCCTAGTGCTAACTGGTTTCATTTACCCAATCGCTGGCA
GCTGGGAATGGAACGGGGGCTGGCTCAACACTGCCTTTGGCGAAGGCGTTGAATTCATTGATTTCGCAGGCTCCTCGATC
GTTCACTCTGTCGGTGCCTGGGCAGGTCTGGTGGGAGCAATGCTGCTCGGTCCGCGCATCGGCAAATTTGTCGGTGGCAA
GGCCCAAGCGATTCCAGGACACAACATGTCCATCGCCACCCTTGGCGCTCTAATCCTCTGGATCGGCTGGTATGGCTTTA
ATCCCGGTTCCCAGCTCGCCATGGATCAATGGGTCCCCTACGTGGCTGTAACAACCACACTCGCAGCTGCAGGTGGCGCC
ATCGGCGCAACGATCATTTCCACCCTCTGCTCCGGCAAGCCTGATCTAACGATGATCATCAACGGCATCCTTGCCGGCCT
AGTGAGCATTACAGCTGGTTGTGGCAATCTCACGCTGGTAGGGGCCTGGGTTGCAGGCCTAATCGGTGGTTTCATTGTGG
TGTTCTCGGTCTCAGCCCTTGACTCTTTAGGCATCGACGATCCAGTTGGAGCTTTCTCTGTCCACGGCATCTGTGGCATC
TGGGGAACCCTCGTGATCGGCCTATGGGGCTTTGACATCCAAGGTGATGGTTCCGCCCTTGGGCTATTCGTTGGGGGTGG
CGCAAGTCAGCTTTGGGCTCAATTTGTAGGCTGCGCTGCCTACGCCATCTGGACCGTGGTGACCTGTTGGATTGCCTGGT
CTGTCATCGGAGCACTGTTTGGCGGTATCCGCGTAACAGAGGCTGAGGAAATCGAAGGTCTTGATATCGGTGAGCACGGT
ATGGAGGCTTATCCCGACTTCGCCTCAGCCGGCAACTAA

Upstream 100 bases:

>100_bases
TTCGTATCAACATGAACATAAAAAAATCCCAAGTTGAGCAAGTTTCTCCTTTGAGCTATCACAAAGGTTTCCAGCTCGTT
TTGCAGATCTGCTTCTTCCA

Downstream 100 bases:

>100_bases
ACCCTGATCAACAACCTGAATCCCATTAAGCCCGACCCTGTGCCGGGCTTTTTTGTGCTTCAAAAGTGTTGAGCTGCCCA
TAGAGTCAGGAGATTGCTGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTTAPLPRSRRRTVRLQEASLLEGPMLLLRSIRGFSSNRSLTWLACVPLALFGLGLFNLSAHAAEMPELNAAFLANNLWL
LVATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFALAVSSYWFIGYSLMYGDPVSAGWLYFNGLFFDPAVTPELIS
EAGLVPSVDFLFQAAFAGTAATIVSGLVAERVKFGEFVVFSLVLTGFIYPIAGSWEWNGGWLNTAFGEGVEFIDFAGSSI
VHSVGAWAGLVGAMLLGPRIGKFVGGKAQAIPGHNMSIATLGALILWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAGGA
IGATIISTLCSGKPDLTMIINGILAGLVSITAGCGNLTLVGAWVAGLIGGFIVVFSVSALDSLGIDDPVGAFSVHGICGI
WGTLVIGLWGFDIQGDGSALGLFVGGGASQLWAQFVGCAAYAIWTVVTCWIAWSVIGALFGGIRVTEAEEIEGLDIGEHG
MEAYPDFASAGN

Sequences:

>Translated_492_residues
MTTAPLPRSRRRTVRLQEASLLEGPMLLLRSIRGFSSNRSLTWLACVPLALFGLGLFNLSAHAAEMPELNAAFLANNLWL
LVATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFALAVSSYWFIGYSLMYGDPVSAGWLYFNGLFFDPAVTPELIS
EAGLVPSVDFLFQAAFAGTAATIVSGLVAERVKFGEFVVFSLVLTGFIYPIAGSWEWNGGWLNTAFGEGVEFIDFAGSSI
VHSVGAWAGLVGAMLLGPRIGKFVGGKAQAIPGHNMSIATLGALILWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAGGA
IGATIISTLCSGKPDLTMIINGILAGLVSITAGCGNLTLVGAWVAGLIGGFIVVFSVSALDSLGIDDPVGAFSVHGICGI
WGTLVIGLWGFDIQGDGSALGLFVGGGASQLWAQFVGCAAYAIWTVVTCWIAWSVIGALFGGIRVTEAEEIEGLDIGEHG
MEAYPDFASAGN
>Mature_491_residues
TTAPLPRSRRRTVRLQEASLLEGPMLLLRSIRGFSSNRSLTWLACVPLALFGLGLFNLSAHAAEMPELNAAFLANNLWLL
VATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFALAVSSYWFIGYSLMYGDPVSAGWLYFNGLFFDPAVTPELISE
AGLVPSVDFLFQAAFAGTAATIVSGLVAERVKFGEFVVFSLVLTGFIYPIAGSWEWNGGWLNTAFGEGVEFIDFAGSSIV
HSVGAWAGLVGAMLLGPRIGKFVGGKAQAIPGHNMSIATLGALILWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAGGAI
GATIISTLCSGKPDLTMIINGILAGLVSITAGCGNLTLVGAWVAGLIGGFIVVFSVSALDSLGIDDPVGAFSVHGICGIW
GTLVIGLWGFDIQGDGSALGLFVGGGASQLWAQFVGCAAYAIWTVVTCWIAWSVIGALFGGIRVTEAEEIEGLDIGEHGM
EAYPDFASAGN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=415, Percent_Identity=34.4578313253012, Blast_Score=178, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI32564042, Length=455, Percent_Identity=34.2857142857143, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=34.331797235023, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=32.0093457943925, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=33.4821428571429, Blast_Score=188, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6325396, Length=426, Percent_Identity=29.1079812206573, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=27.790973871734, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=436, Percent_Identity=29.3577981651376, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078314, Length=427, Percent_Identity=34.8946135831382, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51611; Mature: 51479

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPLPRSRRRTVRLQEASLLEGPMLLLRSIRGFSSNRSLTWLACVPLALFGLGLFNLS
CCCCCCCCCHHHEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCC
AHAAEMPELNAAFLANNLWLLVATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFAL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AVSSYWFIGYSLMYGDPVSAGWLYFNGLFFDPAVTPELISEAGLVPSVDFLFQAAFAGTA
HHHHHHHHHEEEEECCCCCCCEEEEECEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
ATIVSGLVAERVKFGEFVVFSLVLTGFIYPIAGSWEWNGGWLNTAFGEGVEFIDFAGSSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEECHHCCCCHHHHHCCHHH
VHSVGAWAGLVGAMLLGPRIGKFVGGKAQAIPGHNMSIATLGALILWIGWYGFNPGSQLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
MDQWVPYVAVTTTLAAAGGAIGATIISTLCSGKPDLTMIINGILAGLVSITAGCGNLTLV
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
GAWVAGLIGGFIVVFSVSALDSLGIDDPVGAFSVHGICGIWGTLVIGLWGFDIQGDGSAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCEE
GLFVGGGASQLWAQFVGCAAYAIWTVVTCWIAWSVIGALFGGIRVTEAEEIEGLDIGEHG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHCCCCCCCCCC
MEAYPDFASAGN
HHHCCCCCCCCC
>Mature Secondary Structure 
TTAPLPRSRRRTVRLQEASLLEGPMLLLRSIRGFSSNRSLTWLACVPLALFGLGLFNLS
CCCCCCCCHHHEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCC
AHAAEMPELNAAFLANNLWLLVATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFAL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AVSSYWFIGYSLMYGDPVSAGWLYFNGLFFDPAVTPELISEAGLVPSVDFLFQAAFAGTA
HHHHHHHHHEEEEECCCCCCCEEEEECEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
ATIVSGLVAERVKFGEFVVFSLVLTGFIYPIAGSWEWNGGWLNTAFGEGVEFIDFAGSSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEECHHCCCCHHHHHCCHHH
VHSVGAWAGLVGAMLLGPRIGKFVGGKAQAIPGHNMSIATLGALILWIGWYGFNPGSQLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
MDQWVPYVAVTTTLAAAGGAIGATIISTLCSGKPDLTMIINGILAGLVSITAGCGNLTLV
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
GAWVAGLIGGFIVVFSVSALDSLGIDDPVGAFSVHGICGIWGTLVIGLWGFDIQGDGSAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCEE
GLFVGGGASQLWAQFVGCAAYAIWTVVTCWIAWSVIGALFGGIRVTEAEEIEGLDIGEHG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHCCCCCCCCCC
MEAYPDFASAGN
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]