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The map label for this gene is leuD [H]

Identifier: 33864110

GI number: 33864110

Start: 1950686

End: 1951255

Strand: Reverse

Name: leuD [H]

Synonym: PMT1843

Alternate gene names: 33864110

Gene position: 1951255-1950686 (Counterclockwise)

Preceding gene: 33864111

Following gene: 33864109

Centisome position: 80.94

GC content: 52.81

Gene sequence:

>570_bases
ATGTTGCGTGGTGACGACATTGACACGGATCGGATCATCCCAGCCAGGTTTCTTAAATGTGTGAGTTTCGAGGCGCTGGG
AGATCAGGTTTTTGAGGATGATCGCTTGGAACTGGGTGGTTCACATCCCTTCGACCTGAAGGTTCATCAGGGTGCTTCGA
TCTTGGTTGTTAATGATAATTTTGGCTGTGGTTCTAGTCGTGAACATGCACCGCAAGCTTTGATGCGCTGGGGTGTCCGT
GCGCTGATTGGACAGAGCTTTGCAGAAATCTTCTATGGCAACTGTCTAGCGCTGGGAATTCCATGTGCAACTGCCGCCTT
TGCTCAGATTGATGCCTTGCAGCAGGCGGTAGCAGCGGATCCGTCGCAGTGCTGGGGCTTCAATCTTCAGGCTCAGAAGT
TGACGAGTGCTGCGGGTTCATGGGATTTACAGATTGAGTCTGGCCCTCGCGAGATGCTGCTTAGCGGACGTTGGGATGCC
ACATCGCAGTTAGTGGCTCAGGACGCAGCCCTAAAGCGCACGATGGCAGACCTCCCATACCTCAATGATTTTGTGGCGTC
TCTGCATTGA

Upstream 100 bases:

>100_bases
TAATCAGCAACACTGTTCCTTCTAAAAGCTTCCACCAATGACGATTGAGGCTATACCTTTCCCCCATGGCTCTATAGACC
AGGTTCGGGGCCGTGCCGTG

Downstream 100 bases:

>100_bases
ATGTTGTTTGTTGAGGAAGCGGAAGATTGAATTTTTAGTGATTTGCTGCGGCAGATTATTTGCAGCGTGTCTATGAAAAG
AGAAAGCGAGCTGGTCATGG

Product: aconitate hydratase, C-terminal

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVNDNFGCGSSREHAPQALMRWGVR
ALIGQSFAEIFYGNCLALGIPCATAAFAQIDALQQAVAADPSQCWGFNLQAQKLTSAAGSWDLQIESGPREMLLSGRWDA
TSQLVAQDAALKRTMADLPYLNDFVASLH

Sequences:

>Translated_189_residues
MLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVNDNFGCGSSREHAPQALMRWGVR
ALIGQSFAEIFYGNCLALGIPCATAAFAQIDALQQAVAADPSQCWGFNLQAQKLTSAAGSWDLQIESGPREMLLSGRWDA
TSQLVAQDAALKRTMADLPYLNDFVASLH
>Mature_189_residues
MLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVNDNFGCGSSREHAPQALMRWGVR
ALIGQSFAEIFYGNCLALGIPCATAAFAQIDALQQAVAADPSQCWGFNLQAQKLTSAAGSWDLQIESGPREMLLSGRWDA
TSQLVAQDAALKRTMADLPYLNDFVASLH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=180, Percent_Identity=35, Blast_Score=106, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=40.7766990291262, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20556; Mature: 20556

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVNDN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEECCCEEEEEECC
FGCGSSREHAPQALMRWGVRALIGQSFAEIFYGNCLALGIPCATAAFAQIDALQQAVAAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHCC
PSQCWGFNLQAQKLTSAAGSWDLQIESGPREMLLSGRWDATSQLVAQDAALKRTMADLPY
CHHHCCCCCHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCH
LNDFVASLH
HHHHHHHCC
>Mature Secondary Structure
MLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVNDN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEECCCEEEEEECC
FGCGSSREHAPQALMRWGVRALIGQSFAEIFYGNCLALGIPCATAAFAQIDALQQAVAAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHCC
PSQCWGFNLQAQKLTSAAGSWDLQIESGPREMLLSGRWDATSQLVAQDAALKRTMADLPY
CHHHCCCCCHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCH
LNDFVASLH
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]