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The map label for this gene is leuC [H]

Identifier: 33864111

GI number: 33864111

Start: 1951315

End: 1952751

Strand: Reverse

Name: leuC [H]

Synonym: PMT1844

Alternate gene names: 33864111

Gene position: 1952751-1951315 (Counterclockwise)

Preceding gene: 33864112

Following gene: 33864110

Centisome position: 81.0

GC content: 55.6

Gene sequence:

>1437_bases
TTGAGCTCCGGCACCCTCTACGACAAGGTGTGGGATCTCCATCGGGTGGCTGACCTGCCTGGTGGATCCACTCAGTTATT
TGTGGGTTTGCATTTGATCCATGAGGTCACCAGCCCGCAGGCTTTCGCTGCTTTGCAGGACAAGGGTTTGCCGGTGCGGT
GCCCTGAGCGCACCGTGGCGACTGTTGATCACATCGTGCCGACGATTAGTCAGAAGCGTCCGTTTGCTGACCCTTTGGCT
GAGGAGATGCTCAGCACCTTGGAACGAAACTGTGCCAACTACGGGATTGTTCTTTGCGGCCTTGGCAGTGGCCGCCAGGG
CATCGTGCATGTGATAGCTCCTGAGTTGGGGCTGACCCAGCCGGGCATGACTGTGGCTTGTGGTGACTCACACACGTCCA
CTCATGGGGCGTTTGGCGCCATTGCGTTCGGCATCGGCACCAGCCAGGTGCGGGATGTGCTTGCTAGTCAGAGTCTTGCC
ATGAACAAGCTCAAGGTGCGCCGGATCTGGGTCGACGGTCAGCTTGGTTCCGGTGTTTATGCCAAGGATTTGATCCTTCA
TGTGATCCGAAGCCTTGGGGTCAAAGCTGGCGTGGGCTATGCCTATGAGTTCGCCGGACCAGCCATTGATGTGCTCTCTA
TGGAAGAACGCATGACCCTCTGCAATATGGCGATTGAGGGAGGCGCCCGCTGCGGATATGTCAATCCTGATGGGGTCACT
TTTGACTATTTGCAAGGACGGTTTTATGCGCCTACTGGGGAGGCTTTGCATCGGGCAGTGGCCTGGTGGAGGAGCTTGGC
CAGTGATCCAAATGCTGTTTTTGATGATGAGGTGACCTTTGATGCGGCCAGTATTGCGCCCATGGTGACCTGGGGCATCA
CTCCCGGTCAGGGCATTGCTGTAGACGAATCGGTACCGACTGCTGATTCGCTTGAGCCAAGTGAACGTCCAATCTCCGAG
GAAGCTTGTCGCTATATGGATTTGGAGCCGGGAATGGCGATTGAAGGGGTTCCAGTTGATGTTTGTTTTATTGGCAGTTG
CACCAATGGTCGTCTTAGTGATTTAAAGGCTGCTGCCGCTATCGCCAAGGGCCGCCATGTAGCACAAGGGATCAAGGCCT
TTGTGGTGCCAGGATCGGAACAAGTCGCCAGGGCTGCGGAAGCAGAGGGGCTTGATGGTGTTTTTCGGAAGGCTGGATTT
GAATGGCGTGAACCAGGCTGTTCCATGTGTCTGGCAATGAATCCTGATCGTCTTGAGGGGCGTCAGATCAGTGCAAGTTC
CAGTAACAGGAATTTCAAGGGGCGTCAGGGTTCGTCCCGAGGCCGCACCTTGTTGATGAGCCCCGCAATGGTGGCTGCTG
CTGCCATTACCGGTCAGGTCACTGATGTGCGCAAGCTAATCAGCAACACTGTTCCTTCTAAAAGCTTCCACCAATGA

Upstream 100 bases:

>100_bases
AGGTTGGCAATTCAGGAGCTGAGTGTGGTTCGCAGTCTCGATCAGCTGCGCAGGCTCCTGCTGGATGGGAGCTAAGGTTT
TCGGCTACTGCCTCTGAACC

Downstream 100 bases:

>100_bases
CGATTGAGGCTATACCTTTCCCCCATGGCTCTATAGACCAGGTTCGGGGCCGTGCCGTGATGTTGCGTGGTGACGACATT
GACACGGATCGGATCATCCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVATVDHIVPTISQKRPFADPLA
EEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLA
MNKLKVRRIWVDGQLGSGVYAKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
FDYLQGRFYAPTGEALHRAVAWWRSLASDPNAVFDDEVTFDAASIAPMVTWGITPGQGIAVDESVPTADSLEPSERPISE
EACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAAIAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGF
EWREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ

Sequences:

>Translated_478_residues
MSSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVATVDHIVPTISQKRPFADPLA
EEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLA
MNKLKVRRIWVDGQLGSGVYAKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
FDYLQGRFYAPTGEALHRAVAWWRSLASDPNAVFDDEVTFDAASIAPMVTWGITPGQGIAVDESVPTADSLEPSERPISE
EACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAAIAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGF
EWREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ
>Mature_477_residues
SSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVATVDHIVPTISQKRPFADPLAE
EMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAM
NKLKVRRIWVDGQLGSGVYAKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVTF
DYLQGRFYAPTGEALHRAVAWWRSLASDPNAVFDDEVTFDAASIAPMVTWGITPGQGIAVDESVPTADSLEPSERPISEE
ACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAAIAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGFE
WREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=397, Percent_Identity=28.9672544080605, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI8659555, Length=480, Percent_Identity=25.2083333333333, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI41352693, Length=413, Percent_Identity=24.455205811138, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=52.6997840172786, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=27.5482093663912, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564738, Length=443, Percent_Identity=28.6681715575621, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149337, Length=443, Percent_Identity=28.6681715575621, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=321, Percent_Identity=29.595015576324, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=401, Percent_Identity=25.9351620947631, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=50.6382978723404, Blast_Score=453, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322261, Length=404, Percent_Identity=29.4554455445545, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323335, Length=396, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320440, Length=458, Percent_Identity=25.5458515283843, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI281365315, Length=395, Percent_Identity=29.3670886075949, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=395, Percent_Identity=29.3670886075949, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=29.3670886075949, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=394, Percent_Identity=27.4111675126904, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24645686, Length=489, Percent_Identity=25.7668711656442, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.4150943396226, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50892; Mature: 50760

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVA
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHH
TVDHIVPTISQKRPFADPLAEEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAMNKLKVRRIWVDGQLGSGVY
CCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHH
AKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
HHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCE
FDYLQGRFYAPTGEALHRAVAWWRSLASDPNAVFDDEVTFDAASIAPMVTWGITPGQGIA
EHHHCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHCEEEEECCCCCCCEE
VDESVPTADSLEPSERPISEEACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAA
ECCCCCCCCCCCCCCCCCCHHHHHEECCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHH
IAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGFEWREPGCSMCLAMNPDRLEG
HHHCHHHHHCCEEEEECCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVA
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHH
TVDHIVPTISQKRPFADPLAEEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAMNKLKVRRIWVDGQLGSGVY
CCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHH
AKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
HHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCE
FDYLQGRFYAPTGEALHRAVAWWRSLASDPNAVFDDEVTFDAASIAPMVTWGITPGQGIA
EHHHCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHCEEEEECCCCCCCEE
VDESVPTADSLEPSERPISEEACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAA
ECCCCCCCCCCCCCCCCCCHHHHHEECCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHH
IAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGFEWREPGCSMCLAMNPDRLEG
HHHCHHHHHCCEEEEECCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA