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The map label for this gene is slt [H]

Identifier: 33864091

GI number: 33864091

Start: 1932930

End: 1934963

Strand: Direct

Name: slt [H]

Synonym: PMT1824

Alternate gene names: 33864091

Gene position: 1932930-1934963 (Clockwise)

Preceding gene: 33864089

Following gene: 33864093

Centisome position: 80.18

GC content: 56.49

Gene sequence:

>2034_bases
GTGATCGCGAGCCCCTTTCGCGGATTCCCTCTACTCGGAGGTACAGCCCTACTCAGCTTGGCGGCAAGCCTCGCTGGGCA
AGCCCTTTTAAAACCACAGCTCAAGCGACTTACGCCAAGAATGTCGCAAACAGAGCTCTGGCAGGCCTATCGCTGGTCAA
TCGATCCCCAGCAACGCCGAGATGCCTCATTGCTGCTCGTGAGCAAGACAAAAGGATCAACCCTTAGGCATCAGAGGCTG
TTGGCCGGCCAAGGCTGGGGGAACAGCCCCTTAGCAGCCGTTGCCCTCAAGCTGCAAGCCCAAACAGCCGAGCGTCTTGG
GGAAAAATCAAAGGCCAATCATCTTTGGCAAAGCCTGCTGAAACGTTTTCCAAACACGACGACGGCTGCTGATGCGTATT
ACAGCCTCGGGCGCCATAACCCTGAACTGCGTCAGCAACTCCTTCAGCAACATCCTGCTCATCCAGCGGCACTAGCCAGC
GCAATTGAACTGGATACCAGCAACAGTCGCCATCAAAGAGCCATTCATCTCACGCGTTGGGGGGTCTTCTGGCCAGGTGC
CGCAGAAGTCCTACGCAGCGCCTGCAGCGCTCCGGCAAGGAACGGACATTCAAACCAAGAGCGACAAGAACTAGCCCTTG
GTCTTGCACAACTAGGCGACGGCAGAACAGCCCTGAGCTGCCTACAAGAACAACCCCCTGATCCTGCAGCAGCCCTAGCC
ATTGGCCAGACACTGCTACGAGGCAATAAGGAGGAACAACTCCAGGGCGAATCTCTGCTCTTGAAGCTGGCTCAAGATGA
TCCAAAGGCTGCTGAAAGCCTTGAAGCGGTGAGCCTGCTCAGTGAACCTCTTCATCCGAAGGCGACTCTGCTGAAGTCCT
TGCCGCCTGCCCTTGCTGATCGTTCCCCCAGCGTGGCGGCAGCCCGCGTACGCCTCGAAAATGGGATCCAAGCAGACGAG
GTGTTTCAACGCTGGCCTGATAGTCCTGCTAGCTGGCAACTGCAATGGGATCTGAGCCGAGACGCTCTGCTGAAAGAGCA
ATGGAACAAGGCCCAATCGATTCTCGAAAGCATCCCAAGCCAAAAATTGCCGGAGCCCCTCGCCGCTAGGCAACAGTTCT
GGATCGGATTCACTGCCTCCAAACAGGGTCGCAATCGAGAGGCCACCCAGATCTGGGAAAAGCTGCTCCGATCCCATCCC
AATGGGTACTACACCTGGCGCGCCCAGGCACGCCTTGGTCGAGCTGATCTTCCTGCACTGCAAGGGCAACCCGTATCAAT
TGCGAACCCTGAGCTCCCATCCTGGACCCCACTAGGCAGTGGAGAAAAGCTTGTAGACGAACTCTGGCGACTTGGTCTCG
CAATGGAGGCCTGGGAAACGTGGCGGACTCTGAGGGTTGAAGTCGATCGCGCACAACATGATCCCAACACCAGGATGGTG
GAAGGGCGGCTCAGGATGGCCGTCGGCGATAACTGGACGGGGCTAAGTCAGCTCTGGCGAACAAGCCTGCGACTCGTAGG
AGAGGACTGCCACACACGCCAACTGCTCCATCACAGCCAACATCCATATCGCTTTTGGCCTGAGATCAAAAAAGCGAGTC
ACCGCAGTGACGTCCGAGCTGAACTATTGCTGGCCATAGCAAAGCAAGAATCACGCTTCTCGCCAGGCGTGGAGTCTCCA
GTAGGCGCAATAGGGCTGATGCAACTGATGCCGGAAACAGCGGCAGAACTCAGCGATAGCCCTCTGAACAAGCAAGTGCT
GTCCACTCCTGAAACCAATATTGAACTTGGGGCCCGTTACCTAGCAATGCTGCTGAAGCAATGGCAAGGCAATCCATGGC
TGGCAGTGGGCAGTTACAACGCAGGGCCCGCTGCAGTAGCCGACTGGCTAACCCCAGAACTGGCTGCGGACCCTGAGCTC
TGGGTAGAACGAATCCCTTACCCAGAGACACGCCTGTATACAAAAAAAGTTCTCGGCAACCTCTGGAGCTATTTAGGCCT
ATCCAGCAAAAAGCTTTGCGAGACTGAGATGTAA

Upstream 100 bases:

>100_bases
TCAGAGCGGTGAGCCTGAGCAAGCGGCATCAATCCTTGCGAAGACTGCATCACACAACTGAACTTCATCACCACAGAATG
CCAACAGTTTCTGCTCTGGC

Downstream 100 bases:

>100_bases
AGGCATAGAGATCTGCTGGCCAATCCATATCCCGCCAAGCAAAATCAACCCCAGAGCAATCGCCATCACAACCGGTGGTG
GAACCACACGAATGCGAGTG

Product: soluble lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MIASPFRGFPLLGGTALLSLAASLAGQALLKPQLKRLTPRMSQTELWQAYRWSIDPQQRRDASLLLVSKTKGSTLRHQRL
LAGQGWGNSPLAAVALKLQAQTAERLGEKSKANHLWQSLLKRFPNTTTAADAYYSLGRHNPELRQQLLQQHPAHPAALAS
AIELDTSNSRHQRAIHLTRWGVFWPGAAEVLRSACSAPARNGHSNQERQELALGLAQLGDGRTALSCLQEQPPDPAAALA
IGQTLLRGNKEEQLQGESLLLKLAQDDPKAAESLEAVSLLSEPLHPKATLLKSLPPALADRSPSVAAARVRLENGIQADE
VFQRWPDSPASWQLQWDLSRDALLKEQWNKAQSILESIPSQKLPEPLAARQQFWIGFTASKQGRNREATQIWEKLLRSHP
NGYYTWRAQARLGRADLPALQGQPVSIANPELPSWTPLGSGEKLVDELWRLGLAMEAWETWRTLRVEVDRAQHDPNTRMV
EGRLRMAVGDNWTGLSQLWRTSLRLVGEDCHTRQLLHHSQHPYRFWPEIKKASHRSDVRAELLLAIAKQESRFSPGVESP
VGAIGLMQLMPETAAELSDSPLNKQVLSTPETNIELGARYLAMLLKQWQGNPWLAVGSYNAGPAAVADWLTPELAADPEL
WVERIPYPETRLYTKKVLGNLWSYLGLSSKKLCETEM

Sequences:

>Translated_677_residues
MIASPFRGFPLLGGTALLSLAASLAGQALLKPQLKRLTPRMSQTELWQAYRWSIDPQQRRDASLLLVSKTKGSTLRHQRL
LAGQGWGNSPLAAVALKLQAQTAERLGEKSKANHLWQSLLKRFPNTTTAADAYYSLGRHNPELRQQLLQQHPAHPAALAS
AIELDTSNSRHQRAIHLTRWGVFWPGAAEVLRSACSAPARNGHSNQERQELALGLAQLGDGRTALSCLQEQPPDPAAALA
IGQTLLRGNKEEQLQGESLLLKLAQDDPKAAESLEAVSLLSEPLHPKATLLKSLPPALADRSPSVAAARVRLENGIQADE
VFQRWPDSPASWQLQWDLSRDALLKEQWNKAQSILESIPSQKLPEPLAARQQFWIGFTASKQGRNREATQIWEKLLRSHP
NGYYTWRAQARLGRADLPALQGQPVSIANPELPSWTPLGSGEKLVDELWRLGLAMEAWETWRTLRVEVDRAQHDPNTRMV
EGRLRMAVGDNWTGLSQLWRTSLRLVGEDCHTRQLLHHSQHPYRFWPEIKKASHRSDVRAELLLAIAKQESRFSPGVESP
VGAIGLMQLMPETAAELSDSPLNKQVLSTPETNIELGARYLAMLLKQWQGNPWLAVGSYNAGPAAVADWLTPELAADPEL
WVERIPYPETRLYTKKVLGNLWSYLGLSSKKLCETEM
>Mature_677_residues
MIASPFRGFPLLGGTALLSLAASLAGQALLKPQLKRLTPRMSQTELWQAYRWSIDPQQRRDASLLLVSKTKGSTLRHQRL
LAGQGWGNSPLAAVALKLQAQTAERLGEKSKANHLWQSLLKRFPNTTTAADAYYSLGRHNPELRQQLLQQHPAHPAALAS
AIELDTSNSRHQRAIHLTRWGVFWPGAAEVLRSACSAPARNGHSNQERQELALGLAQLGDGRTALSCLQEQPPDPAAALA
IGQTLLRGNKEEQLQGESLLLKLAQDDPKAAESLEAVSLLSEPLHPKATLLKSLPPALADRSPSVAAARVRLENGIQADE
VFQRWPDSPASWQLQWDLSRDALLKEQWNKAQSILESIPSQKLPEPLAARQQFWIGFTASKQGRNREATQIWEKLLRSHP
NGYYTWRAQARLGRADLPALQGQPVSIANPELPSWTPLGSGEKLVDELWRLGLAMEAWETWRTLRVEVDRAQHDPNTRMV
EGRLRMAVGDNWTGLSQLWRTSLRLVGEDCHTRQLLHHSQHPYRFWPEIKKASHRSDVRAELLLAIAKQESRFSPGVESP
VGAIGLMQLMPETAAELSDSPLNKQVLSTPETNIELGARYLAMLLKQWQGNPWLAVGSYNAGPAAVADWLTPELAADPEL
WVERIPYPETRLYTKKVLGNLWSYLGLSSKKLCETEM

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=140, Percent_Identity=40, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75414; Mature: 75414

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASPFRGFPLLGGTALLSLAASLAGQALLKPQLKRLTPRMSQTELWQAYRWSIDPQQRR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCC
DASLLLVSKTKGSTLRHQRLLAGQGWGNSPLAAVALKLQAQTAERLGEKSKANHLWQSLL
CCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KRFPNTTTAADAYYSLGRHNPELRQQLLQQHPAHPAALASAIELDTSNSRHQRAIHLTRW
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHEEEEEEC
GVFWPGAAEVLRSACSAPARNGHSNQERQELALGLAQLGDGRTALSCLQEQPPDPAAALA
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
IGQTLLRGNKEEQLQGESLLLKLAQDDPKAAESLEAVSLLSEPLHPKATLLKSLPPALAD
HHHHHHCCCCHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCC
RSPSVAAARVRLENGIQADEVFQRWPDSPASWQLQWDLSRDALLKEQWNKAQSILESIPS
CCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
QKLPEPLAARQQFWIGFTASKQGRNREATQIWEKLLRSHPNGYYTWRAQARLGRADLPAL
CCCCCHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
QGQPVSIANPELPSWTPLGSGEKLVDELWRLGLAMEAWETWRTLRVEVDRAQHDPNTRMV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCEE
EGRLRMAVGDNWTGLSQLWRTSLRLVGEDCHTRQLLHHSQHPYRFWPEIKKASHRSDVRA
CCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHH
ELLLAIAKQESRFSPGVESPVGAIGLMQLMPETAAELSDSPLNKQVLSTPETNIELGARY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHH
LAMLLKQWQGNPWLAVGSYNAGPAAVADWLTPELAADPELWVERIPYPETRLYTKKVLGN
HHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCHHCCCHHHHHHHCCCCHHHHHHHHHHHH
LWSYLGLSSKKLCETEM
HHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MIASPFRGFPLLGGTALLSLAASLAGQALLKPQLKRLTPRMSQTELWQAYRWSIDPQQRR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCC
DASLLLVSKTKGSTLRHQRLLAGQGWGNSPLAAVALKLQAQTAERLGEKSKANHLWQSLL
CCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KRFPNTTTAADAYYSLGRHNPELRQQLLQQHPAHPAALASAIELDTSNSRHQRAIHLTRW
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHEEEEEEC
GVFWPGAAEVLRSACSAPARNGHSNQERQELALGLAQLGDGRTALSCLQEQPPDPAAALA
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
IGQTLLRGNKEEQLQGESLLLKLAQDDPKAAESLEAVSLLSEPLHPKATLLKSLPPALAD
HHHHHHCCCCHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCC
RSPSVAAARVRLENGIQADEVFQRWPDSPASWQLQWDLSRDALLKEQWNKAQSILESIPS
CCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
QKLPEPLAARQQFWIGFTASKQGRNREATQIWEKLLRSHPNGYYTWRAQARLGRADLPAL
CCCCCHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
QGQPVSIANPELPSWTPLGSGEKLVDELWRLGLAMEAWETWRTLRVEVDRAQHDPNTRMV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCEE
EGRLRMAVGDNWTGLSQLWRTSLRLVGEDCHTRQLLHHSQHPYRFWPEIKKASHRSDVRA
CCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHH
ELLLAIAKQESRFSPGVESPVGAIGLMQLMPETAAELSDSPLNKQVLSTPETNIELGARY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHH
LAMLLKQWQGNPWLAVGSYNAGPAAVADWLTPELAADPELWVERIPYPETRLYTKKVLGN
HHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCHHCCCHHHHHHHCCCCHHHHHHHHHHHH
LWSYLGLSSKKLCETEM
HHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]