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The map label for this gene is pyrB [H]

Identifier: 33864074

GI number: 33864074

Start: 1907751

End: 1908797

Strand: Reverse

Name: pyrB [H]

Synonym: PMT1807

Alternate gene names: 33864074

Gene position: 1908797-1907751 (Counterclockwise)

Preceding gene: 33864075

Following gene: 33864070

Centisome position: 79.17

GC content: 55.3

Gene sequence:

>1047_bases
ATGAGCGGCTGGTCTCATCGTCACATCCTTGATCTGGCCAGCTTCTCCCTTGAGGATTACAGCTCTGTTCTGGAGTTAGC
GCATCGCTTCCGCTCCATGCCAGTCACGGGTGCACGCAAGTTGCCGGCGTTGCAGGGGCGTTTGGTGGCAACGTTGTTTT
TTGAGCCCAGTACGCGTACCCGAAGCAGCTTTGAGTTGGCGGCCCGTCGTCTTTCGGCTGATGTGCAGAGTTTCACTCCA
GCTAGCAGTTCCCTCAGCAAGGGAGAGACTTTGCTTGATACAGCACGCACCTATGTCGCAATGGGCGCTGATGTGCTGGT
GGTGCGTCATGGGTCGACCAGTGTTCCCGAACAACTTGCGTGTGCTTTAGATCGTTCAGGCGAACGCACGGCCGTGCTCA
ATGGTGGCGATGGGTTGCATAGCCATCCCAGCCAGGGATTGCTTGATCTCTACACTCTCGCCCATCATTTTGATCCGGAT
CACCCTCTGCCTGAAGCCATACAAGGCCGAAGGATCGTGATCGTTGGAGATGTCCTTCACTCGCGCGTGGCGCGATCCAA
TCTTTGGGCACTCACTGCTTGCGGTGCTGATGTTGTGTTGTGTGGTCCGCCGAGCCTGGTCCCTCAAGATTTTGTCGCTT
TTGTCGAGGCGCCACCTCCTGGTCAGGCCCATGACCCTGTTCTACATCGTGGTTGTGTGGAGGTGGTGCGAACCCTTGAG
GAGGCGTTGCCTGGTGCGGATGCGGTGATGACTTTGAGGTTGCAGAAGGAAAGGATGCATCAGCATTTGCTTACTGATCT
AAACCGCTTTCATCGTGATTATGGGCTCACCCACGAGCGCCTGAAGTTATGCGGGAAGCCTGTACCTCTGCTCCATCCTG
GGCCTGTGAACAGAGGGGTTGAGTTGGGTGGATCATTACTGGATGATCACTCCATCAGTCTTGTCGAAGAGCAGGTGCGC
AATGGCATTCCTATCCGCATGGCCTTGCTTTATTTGATGGCAGCTTTTGAGTCTTCCCCAGATCCTTCCTTAGAAGCCAT
CGGCTGA

Upstream 100 bases:

>100_bases
GTTGGTATCTCAAGAACTGTCGCAGTATTAGTCGCCGTGCCAAGGGAGATCGCACGCCGCCTTTGCAACAAGCCTGGGCT
CCTGTTGCCAGTGATGGCCA

Downstream 100 bases:

>100_bases
GGAGCTATGCCTGCGATCTGAGTGCTGTAGTACTTCATGGCGGCTTGTAGTGCTGCGTCCGGTGCTGCGATTGAGGGGCC
TAAACCAGGCTGCATTGGGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRTRSSFELAARRLSADVQSFTP
ASSSLSKGETLLDTARTYVAMGADVLVVRHGSTSVPEQLACALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHHFDPD
HPLPEAIQGRRIVIVGDVLHSRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQAHDPVLHRGCVEVVRTLE
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGVELGGSLLDDHSISLVEEQVR
NGIPIRMALLYLMAAFESSPDPSLEAIG

Sequences:

>Translated_348_residues
MSGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRTRSSFELAARRLSADVQSFTP
ASSSLSKGETLLDTARTYVAMGADVLVVRHGSTSVPEQLACALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHHFDPD
HPLPEAIQGRRIVIVGDVLHSRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQAHDPVLHRGCVEVVRTLE
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGVELGGSLLDDHSISLVEEQVR
NGIPIRMALLYLMAAFESSPDPSLEAIG
>Mature_347_residues
SGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRTRSSFELAARRLSADVQSFTPA
SSSLSKGETLLDTARTYVAMGADVLVVRHGSTSVPEQLACALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHHFDPDH
PLPEAIQGRRIVIVGDVLHSRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQAHDPVLHRGCVEVVRTLEE
ALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGVELGGSLLDDHSISLVEEQVRN
GIPIRMALLYLMAAFESSPDPSLEAIG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=325, Percent_Identity=33.2307692307692, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI2367364, Length=338, Percent_Identity=29.8816568047337, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790703, Length=302, Percent_Identity=28.1456953642384, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786469, Length=253, Percent_Identity=30.0395256916996, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=29.3577981651376, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322331, Length=329, Percent_Identity=27.9635258358663, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24642586, Length=334, Percent_Identity=30.2395209580838, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37879; Mature: 37748

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRT
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEEECCCCCC
RSSFELAARRLSADVQSFTPASSSLSKGETLLDTARTYVAMGADVLVVRHGSTSVPEQLA
HHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
CALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHHFDPDHPLPEAIQGRRIVIVGDVLH
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHH
SRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQAHDPVLHRGCVEVVRTLE
HHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGV
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCH
ELGGSLLDDHSISLVEEQVRNGIPIRMALLYLMAAFESSPDPSLEAIG
HHCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRT
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEEECCCCCC
RSSFELAARRLSADVQSFTPASSSLSKGETLLDTARTYVAMGADVLVVRHGSTSVPEQLA
HHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
CALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHHFDPDHPLPEAIQGRRIVIVGDVLH
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHH
SRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQAHDPVLHRGCVEVVRTLE
HHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGV
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCH
ELGGSLLDDHSISLVEEQVRNGIPIRMALLYLMAAFESSPDPSLEAIG
HHCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA