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The map label for this gene is tuf [H]

Identifier: 33864049

GI number: 33864049

Start: 1881556

End: 1882755

Strand: Direct

Name: tuf [H]

Synonym: PMT1782

Alternate gene names: 33864049

Gene position: 1881556-1882755 (Clockwise)

Preceding gene: 33864048

Following gene: 33864050

Centisome position: 78.04

GC content: 54.08

Gene sequence:

>1200_bases
ATGGCTCGCGAGAAGTTTGAGAGGAACAAGCCTCACGTCAACATTGGCACCATCGGCCATGTCGACCACGGCAAAACAAC
CCTGACCGCAGCCATCACAAGTGTGCTTGCGAAAAAGGGTCAAGCCAAGGTGCAAGATTACGCCGAGATCGACGGCGCGC
CTGAAGAGCGTGAGCGCGGTATCACGATCAACACCGCTCACGTGGAATACGAAACCGATGGCCGTCACTATGCACACGTG
GACTGCCCCGGCCACGCGGATTATGTCAAGAACATGATTACCGGTGCGGCCCAGATGGACGGCGCAATCCTTGTTTGCGC
AGCAACCGATGGTCCCATGGCTCAAACCAAGGAGCACATCCTTCTTGCCAAGCAGGTGGGCGTGCCTGCACTGGTTGTCG
CTCTCAACAAATGCGACATGGTCGACGACGAGGAAATTATCGAACTCGTTGAAATGGAGATCCGTGAACTCCTGAGCAGC
TACGACTTCCCTGGTGATGACATCCCCATAGTCCAAGTCTCTGGTCTCAAAGCCATTGAAGGTGAAGCTGAATGGGAAGC
AAAGATCGATGAGTTAATGGAGGCAGTTGATGCATCCATTCCAGAGCCCGAGCGAGAAATCGAAAAACCTTTCCTAATGG
CCGTAGAGGACGTTTTCTCAATCACTGGTCGTGGCACGGTGGCCACCGGACGAATCGAACGCGGCAAGGTCAAAAAAGGC
GAAGAAATTGAAATTGTCGGCATTAGAGACAGCCGCAAGACCACCGTGACCGGTGTGGAAATGTTCCGTAAAGATCTTGA
CGAAGGCTTGGCTGGAGACAATTGCGGGCTCCTACTTCGCGGCATCGAGAAGGAAGACATCGAGCGTGGCATGGTCCTCG
TCAAGCCAGGCTCAATTACACCTCACACTAAATTCGAGGGTCAGGTTTACGTCCTCAAAAAAGAAGAAGGTGGACGCCAC
ACCCCCTTCTTCGCCGGATACCGTCCACAGTTCTACATCCGAACCACTGACGTGACGGGCCAAATTACCGCCTTCACAGC
CGAAGACGGCTCCAATGTGGAGATGGTGATGCCAGGCGACAACATCAAAATGACTGGTGAATTGATCTGCCCTGTGGCCA
TCGAACAGGGCATGCGCTTCGCCATTCGCGAAGGAGGCCGCACCATCGGTGCCGGAGTTGTCTCGAAGATCATCGAATGA

Upstream 100 bases:

>100_bases
GGAAGTTCCTCGCAACGTTGCTGAAGCCATCATCTCCAAGAATCAGGGCAATTCCTGATCTCTATAACACCCACTTTCCA
CCCCCCGATTCTTTTACACA

Downstream 100 bases:

>100_bases
TCAAGAGTGGGATGATTGGGCCTTTGCCTGGTCATCCCTTAAATTCAAAGCTTCAGCCTGCATGAACGATCAACGTTCAA
TGCATCCCCAAAGAACCTCG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITSVLAKKGQAKVQDYAEIDGAPEERERGITINTAHVEYETDGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLSS
YDFPGDDIPIVQVSGLKAIEGEAEWEAKIDELMEAVDASIPEPEREIEKPFLMAVEDVFSITGRGTVATGRIERGKVKKG
EEIEIVGIRDSRKTTVTGVEMFRKDLDEGLAGDNCGLLLRGIEKEDIERGMVLVKPGSITPHTKFEGQVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTAEDGSNVEMVMPGDNIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE

Sequences:

>Translated_399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITSVLAKKGQAKVQDYAEIDGAPEERERGITINTAHVEYETDGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLSS
YDFPGDDIPIVQVSGLKAIEGEAEWEAKIDELMEAVDASIPEPEREIEKPFLMAVEDVFSITGRGTVATGRIERGKVKKG
EEIEIVGIRDSRKTTVTGVEMFRKDLDEGLAGDNCGLLLRGIEKEDIERGMVLVKPGSITPHTKFEGQVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTAEDGSNVEMVMPGDNIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE
>Mature_398_residues
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITSVLAKKGQAKVQDYAEIDGAPEERERGITINTAHVEYETDGRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLSSY
DFPGDDIPIVQVSGLKAIEGEAEWEAKIDELMEAVDASIPEPEREIEKPFLMAVEDVFSITGRGTVATGRIERGKVKKGE
EIEIVGIRDSRKTTVTGVEMFRKDLDEGLAGDNCGLLLRGIEKEDIERGMVLVKPGSITPHTKFEGQVYVLKKEEGGRHT
PFFAGYRPQFYIRTTDVTGQITAFTAEDGSNVEMVMPGDNIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=55.3884711779449, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=29.9319727891156, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=29.9319727891156, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4503475, Length=455, Percent_Identity=28.7912087912088, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=29.0465631929047, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI54607086, Length=387, Percent_Identity=28.6821705426357, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI194018522, Length=435, Percent_Identity=27.816091954023, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI194018520, Length=435, Percent_Identity=27.816091954023, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI194097354, Length=435, Percent_Identity=27.816091954023, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI46094014, Length=436, Percent_Identity=28.6697247706422, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=28.7313432835821, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI157426893, Length=274, Percent_Identity=25.9124087591241, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=237, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI53729337, Length=237, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=72.6130653266332, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=72.6130653266332, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=331, Percent_Identity=33.5347432024169, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=31.5352697095436, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789108, Length=324, Percent_Identity=29.9382716049383, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788922, Length=245, Percent_Identity=28.1632653061224, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=50.3740648379052, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.6410256410256, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=29.8850574712644, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=29.9303944315545, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566629, Length=453, Percent_Identity=28.6975717439294, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=27.1028037383178, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=31.25, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=29.4871794871795, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=26.1648745519713, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17565438, Length=345, Percent_Identity=24.6376811594203, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=177, Percent_Identity=33.8983050847458, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.4760705289673, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=30.2690582959641, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=30.2690582959641, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=450, Percent_Identity=26, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320377, Length=441, Percent_Identity=28.1179138321995, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=27.5, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=29.7520661157025, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=30.1675977653631, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=56.9620253164557, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=56.9620253164557, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=46.2915601023018, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=29.5964125560538, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=29.5964125560538, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=29.5964125560538, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=29.5964125560538, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24652838, Length=445, Percent_Identity=29.8876404494382, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17137572, Length=445, Percent_Identity=29.8876404494382, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.3531353135314, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI17137380, Length=455, Percent_Identity=27.4725274725275, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.5167785234899, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=28.3154121863799, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=28.3154121863799, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78706572, Length=260, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28572034, Length=234, Percent_Identity=31.1965811965812, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582462, Length=156, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43636; Mature: 43505

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITSVLAKKGQAKVQDYAEIDGAPEERERG
CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCC
ITINTAHVEYETDGRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLSSYDFPGDDIPIVQVSGLKAIE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GEAEWEAKIDELMEAVDASIPEPEREIEKPFLMAVEDVFSITGRGTVATGRIERGKVKKG
CCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
EEIEIVGIRDSRKTTVTGVEMFRKDLDEGLAGDNCGLLLRGIEKEDIERGMVLVKPGSIT
CEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCEEEEECCCCC
PHTKFEGQVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTAEDGSNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCC
NIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE
CEEEECEEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITSVLAKKGQAKVQDYAEIDGAPEERERG
CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCC
ITINTAHVEYETDGRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEIIELVEMEIRELLSSYDFPGDDIPIVQVSGLKAIE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GEAEWEAKIDELMEAVDASIPEPEREIEKPFLMAVEDVFSITGRGTVATGRIERGKVKKG
CCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
EEIEIVGIRDSRKTTVTGVEMFRKDLDEGLAGDNCGLLLRGIEKEDIERGMVLVKPGSIT
CEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCEEEEECCCCC
PHTKFEGQVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTAEDGSNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCC
NIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVSKIIE
CEEEECEEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA