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The map label for this gene is fusA [H]

Identifier: 33864048

GI number: 33864048

Start: 1879438

End: 1881513

Strand: Direct

Name: fusA [H]

Synonym: PMT1781

Alternate gene names: 33864048

Gene position: 1879438-1881513 (Clockwise)

Preceding gene: 33864047

Following gene: 33864049

Centisome position: 77.96

GC content: 52.6

Gene sequence:

>2076_bases
GTGGCTCGCGCCTTTCCCCTGGAACGCGTCAGAAATATCGGGATTGCCGCCCATATTGACGCCGGCAAAACCACCTGTAC
GGAACGCATCCTTTTCTATTCCGGTGTGGTTCACAAGATGGGCGAGGTGCATGACGGCGCCGCAGTCACCGACTGGATGG
CTCAGGAGCGCGAGCGCGGAATCACCATTACAGCGGCTGCGATCTCGACAACCTGGAACGATCACCGCATCAATATCATT
GACACCCCAGGGCACGTTGACTTCACCATTGAAGTGGAGCGCTCGATGCGAGTGCTCGATGGTGTAATCGCCGTGTTTTG
TGCGGTAGGTGGCGTGCAACCTCAATCAGAAACCGTCTGGCGGCAAGCCGATCGCTATTCCGTTCCACGGATGGTTTTTG
TCAACAAAATGGACCGCACCGGGGCCGACTTCCTCAAGGTTCACGGACAGATCAAGAATCGCCTCAAAGCCAACGCCATC
CCAATCCAGCTGCCTATTGGTGCAGAGGGAGATCTCAGCGGCATTATTGACCTCGTTAAAAACAAAGCCTTTATTTATAA
GGACGATCTCGGCAAGGAAATCGAAGAAACAGAGATCCCAGACCATATGAAAGAGATTGCAGCTGAATGGCGATCCAAGC
TGATGGAATGTGTTGCTGAAACCGACGAAGAACTGATTGAAGTTTTTCTAGAGACAGAAGAACTAAGCGAAGCCCAACTT
GCATCAGGCATTCGCGAAGGGGTTCTCAATCACGGCCTAGTACCTTTGCTGTGTGGATCTGCCTTCAAGAACAAGGGCGT
TCAACTGCTATTGGATGCAGTGGTGGACTACCTACCCGCTCCTGTTGATGTACCCCCGATTCAAGGTTTACTTCCCAATG
GCAAGGAGGCAGTAAGGCCATCCGATGACAACGCACCATTTAGTGCTTTGGCCTTCAAGGTGATGTCGGACCCCTACGGC
AAGCTCACCTTCGTGCGTATGTATTCAGGGGTGCTCGAGAAAGGCAGTTATGTGCTGAACTCCACGAAGAACGAGAAAGA
ACGAATTTCCCGACTGATCATTCTCAAGGCCGATGACCGTGAAGAGGTCGATGCACTACGCGCTGGAGATCTCGGTGCTG
TTCTAGGTCTGAAGAACACCACGACAGGCGACACCCTTTGCACGACTGACGATCCAATTGTTCTAGAAACCCTCTACATC
CCCGAGCCAGTGATATCGGTGGCTGTTGAGCCCAAAACCAAGGGTGATATGGAAAAACTTTCCAAAGCCCTGCTTTCATT
AGCGGAAGAGGATCCCACCTTCCGCGTCAGCACAGACCCTGAGACCAGTCAAACCGTGATTGCTGGTATGGGCGAACTCC
ACCTTGAAATTCTCGTGGATCGCATGCTGCGAGAATTCAAGGTGGAGGCCAACATCGGCGCTCCTCAGGTCTCCTACAGG
GAAACCATTCGTGCCAGCTCTAAAGGCGAGGGCAAGTTTGCCCGTCAAACAGGTGGTAAAGGCCAATACGGCCACGTAGT
AATCGAAATGGAGCCAGGCGAACCTGGTTCTGGCTTTGAATTCGTGAACAAGATTGTCGGAGGCATCGTTCCCAAGGAAT
ACATCAAGCCTGCCGAATCAGGCATGCGTGAAACATGCGAATCCGGCGTTATCGCTGGCTATCCGCTCATCGATGTGAAA
GTCACCATGGTTGACGGGTCTTACCACGATGTGGACTCATCCGAGATGGCTTTCAAAATCGCCGGATCGATGGCTTTCAA
AGATGGCGTCAAGAAGTGCAATCCTGTGCTGCTTGAACCGATGATGAAGGTTGAGGTCGAGATCCCCGAGGATTTCCTCG
GTGCGATCATCGGCGATCTGTCCTCCCGGCGAGGTCAGGTTGAAGGCCAGTCCATTGACGATGGACTCTCTAAAGTGCAG
TCCAAAGTGCCTCTAGCCGAAATGTTCGGTTACGCCACCCAGCTCCGATCTATGACTCAGGGTCGGGGTATCTTCTCGAT
GGAGTTCAGCCATTACGAGGAAGTTCCTCGCAACGTTGCTGAAGCCATCATCTCCAAGAATCAGGGCAATTCCTGA

Upstream 100 bases:

>100_bases
GCTACTGAACATGGCCAAGCCCTGACGGCACTGAACCGTTCCAACGGCTGACCTGTAGAGTCGCACCCGCCTTTTAACGC
CAAACCCCGGAGAGTTTCCT

Downstream 100 bases:

>100_bases
TCTCTATAACACCCACTTTCCACCCCCCGATTCTTTTACACAATGGCTCGCGAGAAGTTTGAGAGGAACAAGCCTCACGT
CAACATTGGCACCATCGGCC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARAFPLERVRNIGIAAHIDAGKTTCTERILFYSGVVHKMGEVHDGAAVTDWMAQERERGITITAAAISTTWNDHRINII
DTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVWRQADRYSVPRMVFVNKMDRTGADFLKVHGQIKNRLKANAI
PIQLPIGAEGDLSGIIDLVKNKAFIYKDDLGKEIEETEIPDHMKEIAAEWRSKLMECVAETDEELIEVFLETEELSEAQL
ASGIREGVLNHGLVPLLCGSAFKNKGVQLLLDAVVDYLPAPVDVPPIQGLLPNGKEAVRPSDDNAPFSALAFKVMSDPYG
KLTFVRMYSGVLEKGSYVLNSTKNEKERISRLIILKADDREEVDALRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLYI
PEPVISVAVEPKTKGDMEKLSKALLSLAEEDPTFRVSTDPETSQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
ETIRASSKGEGKFARQTGGKGQYGHVVIEMEPGEPGSGFEFVNKIVGGIVPKEYIKPAESGMRETCESGVIAGYPLIDVK
VTMVDGSYHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEPMMKVEVEIPEDFLGAIIGDLSSRRGQVEGQSIDDGLSKVQ
SKVPLAEMFGYATQLRSMTQGRGIFSMEFSHYEEVPRNVAEAIISKNQGNS

Sequences:

>Translated_691_residues
MARAFPLERVRNIGIAAHIDAGKTTCTERILFYSGVVHKMGEVHDGAAVTDWMAQERERGITITAAAISTTWNDHRINII
DTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVWRQADRYSVPRMVFVNKMDRTGADFLKVHGQIKNRLKANAI
PIQLPIGAEGDLSGIIDLVKNKAFIYKDDLGKEIEETEIPDHMKEIAAEWRSKLMECVAETDEELIEVFLETEELSEAQL
ASGIREGVLNHGLVPLLCGSAFKNKGVQLLLDAVVDYLPAPVDVPPIQGLLPNGKEAVRPSDDNAPFSALAFKVMSDPYG
KLTFVRMYSGVLEKGSYVLNSTKNEKERISRLIILKADDREEVDALRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLYI
PEPVISVAVEPKTKGDMEKLSKALLSLAEEDPTFRVSTDPETSQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
ETIRASSKGEGKFARQTGGKGQYGHVVIEMEPGEPGSGFEFVNKIVGGIVPKEYIKPAESGMRETCESGVIAGYPLIDVK
VTMVDGSYHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEPMMKVEVEIPEDFLGAIIGDLSSRRGQVEGQSIDDGLSKVQ
SKVPLAEMFGYATQLRSMTQGRGIFSMEFSHYEEVPRNVAEAIISKNQGNS
>Mature_690_residues
ARAFPLERVRNIGIAAHIDAGKTTCTERILFYSGVVHKMGEVHDGAAVTDWMAQERERGITITAAAISTTWNDHRINIID
TPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVWRQADRYSVPRMVFVNKMDRTGADFLKVHGQIKNRLKANAIP
IQLPIGAEGDLSGIIDLVKNKAFIYKDDLGKEIEETEIPDHMKEIAAEWRSKLMECVAETDEELIEVFLETEELSEAQLA
SGIREGVLNHGLVPLLCGSAFKNKGVQLLLDAVVDYLPAPVDVPPIQGLLPNGKEAVRPSDDNAPFSALAFKVMSDPYGK
LTFVRMYSGVLEKGSYVLNSTKNEKERISRLIILKADDREEVDALRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLYIP
EPVISVAVEPKTKGDMEKLSKALLSLAEEDPTFRVSTDPETSQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYRE
TIRASSKGEGKFARQTGGKGQYGHVVIEMEPGEPGSGFEFVNKIVGGIVPKEYIKPAESGMRETCESGVIAGYPLIDVKV
TMVDGSYHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEPMMKVEVEIPEDFLGAIIGDLSSRRGQVEGQSIDDGLSKVQS
KVPLAEMFGYATQLRSMTQGRGIFSMEFSHYEEVPRNVAEAIISKNQGNS

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=694, Percent_Identity=43.2276657060519, Blast_Score=577, Evalue=1e-164,
Organism=Homo sapiens, GI19923640, Length=730, Percent_Identity=39.4520547945205, Blast_Score=477, Evalue=1e-134,
Organism=Homo sapiens, GI25306287, Length=729, Percent_Identity=37.3113854595336, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI25306283, Length=445, Percent_Identity=43.1460674157303, Blast_Score=318, Evalue=8e-87,
Organism=Homo sapiens, GI157426893, Length=156, Percent_Identity=38.4615384615385, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI4503483, Length=474, Percent_Identity=24.8945147679325, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=38.0597014925373, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=38.135593220339, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=38.135593220339, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=38.135593220339, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1789738, Length=699, Percent_Identity=60.9442060085837, Blast_Score=873, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=473, Percent_Identity=28.1183932346723, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=41.1428571428571, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=35.5263157894737, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=680, Percent_Identity=42.0588235294118, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17556745, Length=694, Percent_Identity=27.6657060518732, Blast_Score=273, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17506493, Length=487, Percent_Identity=25.8726899383984, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=154, Percent_Identity=38.3116883116883, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=31.3432835820896, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=31.3432835820896, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17552882, Length=135, Percent_Identity=34.0740740740741, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=690, Percent_Identity=41.8840579710145, Blast_Score=558, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6322359, Length=794, Percent_Identity=33.2493702770781, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324707, Length=148, Percent_Identity=40.5405405405405, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=148, Percent_Identity=40.5405405405405, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=156, Percent_Identity=36.5384615384615, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=32.8767123287671, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=694, Percent_Identity=43.8040345821326, Blast_Score=594, Evalue=1e-170,
Organism=Drosophila melanogaster, GI221458488, Length=725, Percent_Identity=33.2413793103448, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24585711, Length=486, Percent_Identity=25.3086419753086, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585713, Length=486, Percent_Identity=25.3086419753086, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585709, Length=486, Percent_Identity=25.3086419753086, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI78706572, Length=144, Percent_Identity=38.1944444444444, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574573, Length=141, Percent_Identity=36.1702127659575, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21357743, Length=137, Percent_Identity=31.3868613138686, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75639; Mature: 75508

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAFPLERVRNIGIAAHIDAGKTTCTERILFYSGVVHKMGEVHDGAAVTDWMAQERERG
CCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
ITITAAAISTTWNDHRINIIDTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVW
EEEEEEEEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RQADRYSVPRMVFVNKMDRTGADFLKVHGQIKNRLKANAIPIQLPIGAEGDLSGIIDLVK
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
NKAFIYKDDLGKEIEETEIPDHMKEIAAEWRSKLMECVAETDEELIEVFLETEELSEAQL
CCCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGIREGVLNHGLVPLLCGSAFKNKGVQLLLDAVVDYLPAPVDVPPIQGLLPNGKEAVRP
HHHHHHHHHHCCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SDDNAPFSALAFKVMSDPYGKLTFVRMYSGVLEKGSYVLNSTKNEKERISRLIILKADDR
CCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCEEECCCCCHHHHCCEEEEEECCCH
EEVDALRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLYIPEPVISVAVEPKTKGDMEKL
HHHHHHHCCCCCEEEECCCCCCCCEEECCCCCEEEEEEECCCCHHEEEECCCCCCCHHHH
SKALLSLAEEDPTFRVSTDPETSQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ETIRASSKGEGKFARQTGGKGQYGHVVIEMEPGEPGSGFEFVNKIVGGIVPKEYIKPAES
HHHHCCCCCCCCCHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCHHHH
GMRETCESGVIAGYPLIDVKVTMVDGSYHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEP
HHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHCC
MMKVEVEIPEDFLGAIIGDLSSRRGQVEGQSIDDGLSKVQSKVPLAEMFGYATQLRSMTQ
CCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRGIFSMEFSHYEEVPRNVAEAIISKNQGNS
CCCEEEECHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARAFPLERVRNIGIAAHIDAGKTTCTERILFYSGVVHKMGEVHDGAAVTDWMAQERERG
CCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
ITITAAAISTTWNDHRINIIDTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVW
EEEEEEEEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RQADRYSVPRMVFVNKMDRTGADFLKVHGQIKNRLKANAIPIQLPIGAEGDLSGIIDLVK
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
NKAFIYKDDLGKEIEETEIPDHMKEIAAEWRSKLMECVAETDEELIEVFLETEELSEAQL
CCCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGIREGVLNHGLVPLLCGSAFKNKGVQLLLDAVVDYLPAPVDVPPIQGLLPNGKEAVRP
HHHHHHHHHHCCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SDDNAPFSALAFKVMSDPYGKLTFVRMYSGVLEKGSYVLNSTKNEKERISRLIILKADDR
CCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCEEECCCCCHHHHCCEEEEEECCCH
EEVDALRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLYIPEPVISVAVEPKTKGDMEKL
HHHHHHHCCCCCEEEECCCCCCCCEEECCCCCEEEEEEECCCCHHEEEECCCCCCCHHHH
SKALLSLAEEDPTFRVSTDPETSQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ETIRASSKGEGKFARQTGGKGQYGHVVIEMEPGEPGSGFEFVNKIVGGIVPKEYIKPAES
HHHHCCCCCCCCCHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCHHHH
GMRETCESGVIAGYPLIDVKVTMVDGSYHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEP
HHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHCC
MMKVEVEIPEDFLGAIIGDLSSRRGQVEGQSIDDGLSKVQSKVPLAEMFGYATQLRSMTQ
CCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRGIFSMEFSHYEEVPRNVAEAIISKNQGNS
CCCEEEECHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA