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The map label for this gene is lipA

Identifier: 33864042

GI number: 33864042

Start: 1870538

End: 1871422

Strand: Direct

Name: lipA

Synonym: PMT1775

Alternate gene names: 33864042

Gene position: 1870538-1871422 (Clockwise)

Preceding gene: 33864039

Following gene: 33864045

Centisome position: 77.59

GC content: 56.5

Gene sequence:

>885_bases
ATGACTGGAAAAGCCTTACTGAAACCCCACTGGTTGCGCGTCAAGGCTCCACAACAGCAACGCATCGGCGAGGTAGCCGA
GCTGCTGAAGGGCCTCAATCTCAAGACGGTGTGTCAAGAAGCCAGCTGCCCGAATATCGGGGAGTGCTTCGCAGGAGGAA
CTGCCACCTTCTTGATCATGGGGCCGGGCTGCACCCGCGCTTGCCCTTACTGCGACATCGACTTTGACAAAAGCCCACGC
TCCCTAGACCCCACTGAGCCAGAGCGCCTAAGCGAAGCGGTGGCTCAGATGAACCTTCGTCATGTTGTCATCACTTCAGT
TAACCGTGATGACCTCAGCGACGGGGGAGCCAGTCAATTCGTCGCATGCATCGAGGCCATTCGCCAACGTTCTCCCCTAA
CCACAATTGAGCTGCTAATTCCTGATTTCAACGGCAATTGGGATGCCCTCGCCAAGGTAATGGGAGCAGCTCCGGAGGTG
CTCAACCACAACATTGAGACCGTCCCCCGGCTCTACAAACGGGCTCGTCCACAAGCTGACTACATGCGATCTCTTGAGCT
GTTGTATCAAGTGCGGCAAGGCTGGCCGAAGGTCTATAGCAAGTCCGGCCTGATGGTGGGGCTAGGTGAAACAGATGCTG
AGGTAATTGAGGTCCTAGAGGATCTACGCCGTCACCGAGTCGACATAGTGACCATCGGCCAATACCTATCGCCAAGCCCA
AAACATCTTCCAGTCGATCGCTTTGTGACACCAGAACAGTTCGAATATTTCCGCTCTATGGGGGAAACTGAGCTGGGGTT
CCTGCAAGTGGTTAGTACCCCTCTCACACGCAGCAGCTACCACGCCGGCGAGGTCCAGCGGCTGATGGCCGCCCACCCAC
GCTGA

Upstream 100 bases:

>100_bases
ACGAAGTGCGCTACCAAGCTGCGCTACATCCCGATAGGCTCACTGTAGAAGGTGAAGTCGCCTGGAACTGCTGCTGCCAG
TGGGTTCCTGAACTGCTCTC

Downstream 100 bases:

>100_bases
ACTCAAGCCATGAGCTCCCCACACAGAGGGATCCACTGCTGCAGATCCCATTTAACTAAACCCTCAACAATCATCGGGTC
CTGTTCGACTAAGCGTTTGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTGKALLKPHWLRVKAPQQQRIGEVAELLKGLNLKTVCQEASCPNIGECFAGGTATFLIMGPGCTRACPYCDIDFDKSPR
SLDPTEPERLSEAVAQMNLRHVVITSVNRDDLSDGGASQFVACIEAIRQRSPLTTIELLIPDFNGNWDALAKVMGAAPEV
LNHNIETVPRLYKRARPQADYMRSLELLYQVRQGWPKVYSKSGLMVGLGETDAEVIEVLEDLRRHRVDIVTIGQYLSPSP
KHLPVDRFVTPEQFEYFRSMGETELGFLQVVSTPLTRSSYHAGEVQRLMAAHPR

Sequences:

>Translated_294_residues
MTGKALLKPHWLRVKAPQQQRIGEVAELLKGLNLKTVCQEASCPNIGECFAGGTATFLIMGPGCTRACPYCDIDFDKSPR
SLDPTEPERLSEAVAQMNLRHVVITSVNRDDLSDGGASQFVACIEAIRQRSPLTTIELLIPDFNGNWDALAKVMGAAPEV
LNHNIETVPRLYKRARPQADYMRSLELLYQVRQGWPKVYSKSGLMVGLGETDAEVIEVLEDLRRHRVDIVTIGQYLSPSP
KHLPVDRFVTPEQFEYFRSMGETELGFLQVVSTPLTRSSYHAGEVQRLMAAHPR
>Mature_293_residues
TGKALLKPHWLRVKAPQQQRIGEVAELLKGLNLKTVCQEASCPNIGECFAGGTATFLIMGPGCTRACPYCDIDFDKSPRS
LDPTEPERLSEAVAQMNLRHVVITSVNRDDLSDGGASQFVACIEAIRQRSPLTTIELLIPDFNGNWDALAKVMGAAPEVL
NHNIETVPRLYKRARPQADYMRSLELLYQVRQGWPKVYSKSGLMVGLGETDAEVIEVLEDLRRHRVDIVTIGQYLSPSPK
HLPVDRFVTPEQFEYFRSMGETELGFLQVVSTPLTRSSYHAGEVQRLMAAHPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=288, Percent_Identity=42.3611111111111, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=40.8, Blast_Score=212, Evalue=2e-55,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=50.179211469534, Blast_Score=292, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=255, Percent_Identity=45.0980392156863, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=40.4255319148936, Blast_Score=233, Evalue=2e-62,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=42.756183745583, Blast_Score=240, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA1_PROMM (Q7V507)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895602.1
- ProteinModelPortal:   Q7V507
- STRING:   Q7V507
- GeneID:   1728731
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1775
- NMPDR:   fig|74547.1.peg.1769
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   PMAR74547:PMT1775-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32818; Mature: 32687

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKALLKPHWLRVKAPQQQRIGEVAELLKGLNLKTVCQEASCPNIGECFAGGTATFLIM
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHCCCCEEEEEE
GPGCTRACPYCDIDFDKSPRSLDPTEPERLSEAVAQMNLRHVVITSVNRDDLSDGGASQF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHH
VACIEAIRQRSPLTTIELLIPDFNGNWDALAKVMGAAPEVLNHNIETVPRLYKRARPQAD
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCHHH
YMRSLELLYQVRQGWPKVYSKSGLMVGLGETDAEVIEVLEDLRRHRVDIVTIGQYLSPSP
HHHHHHHHHHHHHCCHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECHHHCCCC
KHLPVDRFVTPEQFEYFRSMGETELGFLQVVSTPLTRSSYHAGEVQRLMAAHPR
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TGKALLKPHWLRVKAPQQQRIGEVAELLKGLNLKTVCQEASCPNIGECFAGGTATFLIM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHCCCCEEEEEE
GPGCTRACPYCDIDFDKSPRSLDPTEPERLSEAVAQMNLRHVVITSVNRDDLSDGGASQF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHH
VACIEAIRQRSPLTTIELLIPDFNGNWDALAKVMGAAPEVLNHNIETVPRLYKRARPQAD
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCHHH
YMRSLELLYQVRQGWPKVYSKSGLMVGLGETDAEVIEVLEDLRRHRVDIVTIGQYLSPSP
HHHHHHHHHHHHHCCHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECHHHCCCC
KHLPVDRFVTPEQFEYFRSMGETELGFLQVVSTPLTRSSYHAGEVQRLMAAHPR
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642