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The map label for this gene is prfA

Identifier: 33864028

GI number: 33864028

Start: 1851246

End: 1852343

Strand: Direct

Name: prfA

Synonym: PMT1761

Alternate gene names: 33864028

Gene position: 1851246-1852343 (Clockwise)

Preceding gene: 33864027

Following gene: 33864030

Centisome position: 76.79

GC content: 54.83

Gene sequence:

>1098_bases
ATGGACTCTTCAACCCTCATCACTCGGCTGGAAACAGCCAAGACCAGTTTTCAAAGTCTGGAGCGCCAGCTAGCTGATCC
CGATGTGGCAGCTGATCCCAAACTTCTTGAAAGTATTGCGCGAGAAAGAGCACGACTGGAACCCCTCGTACTCGATTTTG
AAGCCCTCCAGCATGTGGAGCAGGAATGGCAAGAGACCAAGCAGCTACTACGAGAAAGTAGGGGTGATGACGCCATGGAG
TCTTTAGCCCAGGAAGAGTTGCAACAACTAGAGGCACGCAAAACAGTCTTGGTGAGACGCCTCACCCTGGCCCTACTCCC
AAGTGATCCTCGTGATCAACGCAGCGTCATGCTGGAGATCCGCGCCGGTGCAGGCGGAAACGAAGCCTGCCTATGGGCAG
GTGATTTAGCCCGCATGTATGAGCGCTACGGTCAACGCATTGGCTGGTCAGTCCAACCGTTGAGTGCAACAGAGGCCGAT
TTGGGGGGATTCCGTGAGCTCATCCTCTCAATCAAAGGCGATGCTGTCTTCAGCCAACTGAAATTCGAAGCCGGCGTGCA
TCGAGTTCAACGAGTTCCTGCTACAGAATCCCAAGGTCGAGTGCACACCTCCACAGCAACAGTGGCTGTGATGCCAGAAG
CTGATGCAGTGGAAGTGCAGATCGATCCCAGCGAACTAGAAATCAGCACTGCTCGCTCAGGCGGGGCTGGCGGACAAAAT
GTCAACAAGGTTGAAACGGCAGTGGACCTGCTCCACAAGCCCACCGGTATCAGGGTGTTCTGCACCCAGCAACGCTCCCA
ACTGCAGAACCGAGAACGTGCCATGGAAATCCTGAGAGCGAAGTTGCTGGAAAGACAAATTGCAGAAGCCAACGCGCGTG
AAAGCTCAACGAGAAGAGCCCAAGTGGGAACTGGCGACCGCAGCGAAAAGATCCGCACCTACAACTACAAAGACAATCGC
ACCACTGACCACCGATTAGGACGCAATTTCTCCCTCGAGCCAGTGCTGACTGGCCAGCTGGAAGAGCTGATCGGCGCCTG
CATTGCCGAAGAGCAACGCCATCAACTGGAAGAACTCAGTCATCAGGAGGAAGACTGA

Upstream 100 bases:

>100_bases
AGACGCCAAAAACAACAACAAGGACAACACCTCTAAAGAAGACTGAGCTGTTGTCCTCAAAGACTTCGCCGGGTGCCAAT
AACATCCGCTGTTGATGGGT

Downstream 100 bases:

>100_bases
CATTGAGGCTGGTATCACCAGAGCCCCAGCCGATTACATACTTTTCCCACTCGCTGTGTCGGCCCGAGTGAATCTGTCGA
GTCGCCTCAAAACTGAGACT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MDSSTLITRLETAKTSFQSLERQLADPDVAADPKLLESIARERARLEPLVLDFEALQHVEQEWQETKQLLRESRGDDAME
SLAQEELQQLEARKTVLVRRLTLALLPSDPRDQRSVMLEIRAGAGGNEACLWAGDLARMYERYGQRIGWSVQPLSATEAD
LGGFRELILSIKGDAVFSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADAVEVQIDPSELEISTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTQQRSQLQNRERAMEILRAKLLERQIAEANARESSTRRAQVGTGDRSEKIRTYNYKDNR
TTDHRLGRNFSLEPVLTGQLEELIGACIAEEQRHQLEELSHQEED

Sequences:

>Translated_365_residues
MDSSTLITRLETAKTSFQSLERQLADPDVAADPKLLESIARERARLEPLVLDFEALQHVEQEWQETKQLLRESRGDDAME
SLAQEELQQLEARKTVLVRRLTLALLPSDPRDQRSVMLEIRAGAGGNEACLWAGDLARMYERYGQRIGWSVQPLSATEAD
LGGFRELILSIKGDAVFSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADAVEVQIDPSELEISTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTQQRSQLQNRERAMEILRAKLLERQIAEANARESSTRRAQVGTGDRSEKIRTYNYKDNR
TTDHRLGRNFSLEPVLTGQLEELIGACIAEEQRHQLEELSHQEED
>Mature_365_residues
MDSSTLITRLETAKTSFQSLERQLADPDVAADPKLLESIARERARLEPLVLDFEALQHVEQEWQETKQLLRESRGDDAME
SLAQEELQQLEARKTVLVRRLTLALLPSDPRDQRSVMLEIRAGAGGNEACLWAGDLARMYERYGQRIGWSVQPLSATEAD
LGGFRELILSIKGDAVFSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADAVEVQIDPSELEISTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTQQRSQLQNRERAMEILRAKLLERQIAEANARESSTRRAQVGTGDRSEKIRTYNYKDNR
TTDHRLGRNFSLEPVLTGQLEELIGACIAEEQRHQLEELSHQEED

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=308, Percent_Identity=41.2337662337662, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI34577120, Length=325, Percent_Identity=36.9230769230769, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI166795305, Length=216, Percent_Identity=39.8148148148148, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=44.3213296398892, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=32.7536231884058, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=266, Percent_Identity=37.593984962406, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=38.2636655948553, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI19921226, Length=324, Percent_Identity=39.8148148148148, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PROMM (Q7V518)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895588.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q7V518
- SMR:   Q7V518
- STRING:   Q7V518
- GeneID:   1729137
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1761
- NMPDR:   fig|74547.1.peg.1755
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   PMAR74547:PMT1761-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40916; Mature: 40916

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSTLITRLETAKTSFQSLERQLADPDVAADPKLLESIARERARLEPLVLDFEALQHVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEECHHHHHHHH
QEWQETKQLLRESRGDDAMESLAQEELQQLEARKTVLVRRLTLALLPSDPRDQRSVMLEI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
RAGAGGNEACLWAGDLARMYERYGQRIGWSVQPLSATEADLGGFRELILSIKGDAVFSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADAVEVQIDPSELEISTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCC
VNKVETAVDLLHKPTGIRVFCTQQRSQLQNRERAMEILRAKLLERQIAEANARESSTRRA
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
QVGTGDRSEKIRTYNYKDNRTTDHRLGRNFSLEPVLTGQLEELIGACIAEEQRHQLEELS
CCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HQEED
CCCCC
>Mature Secondary Structure
MDSSTLITRLETAKTSFQSLERQLADPDVAADPKLLESIARERARLEPLVLDFEALQHVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEECHHHHHHHH
QEWQETKQLLRESRGDDAMESLAQEELQQLEARKTVLVRRLTLALLPSDPRDQRSVMLEI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
RAGAGGNEACLWAGDLARMYERYGQRIGWSVQPLSATEADLGGFRELILSIKGDAVFSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADAVEVQIDPSELEISTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCC
VNKVETAVDLLHKPTGIRVFCTQQRSQLQNRERAMEILRAKLLERQIAEANARESSTRRA
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
QVGTGDRSEKIRTYNYKDNRTTDHRLGRNFSLEPVLTGQLEELIGACIAEEQRHQLEELS
CCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HQEED
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642