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The map label for this gene is recF

Identifier: 33863982

GI number: 33863982

Start: 1817124

End: 1818221

Strand: Direct

Name: recF

Synonym: PMT1715

Alternate gene names: 33863982

Gene position: 1817124-1818221 (Clockwise)

Preceding gene: 33863981

Following gene: 33863983

Centisome position: 75.37

GC content: 56.65

Gene sequence:

>1098_bases
CTGCAGGGGTATCGCAATTACAGCAGGCTTCAGCTGGAGTTGACGGAAAATCGGCTGCTGGTCATTGGCCCCAACGGCAT
CGGCAAATCCAACCTTCTCGAAGCCATAGAGCTACTCGGCAGCCTGCGCTCGCATCGCGCCAGCAGCGATCAAGACTTGA
TCCACTGGGAAGAACAGCGGGCCCTACTTCGCGCCATCGCTGATGACACTGAGAAGCTCGAACTGGAATTGCGGCGGCAA
GGAGGTCGACAAGCCCGCCGCAACGGCAAAACGCTCACCCGCCAATTGGATCTAATAGGTCCACTGCGTTGCGTGGGGTT
TAGCGCTCTCGATCTCAATCTCGTTCGAGGCGAGCCTGCCCTGCGGCGCCAATGGCTTGATCGCGTCGTACAACAACTTG
AACCGATCTATTCAGATCTGATCAGTCGCTTCAACAAGCTGCTGCGTCAGCGAAGCCAGCTGTGGCGTCAGTGGCGCCAC
ATCCCGATTCAGGAGCGTGATTCCCTCTTGGATGCCTTCGATGTACAGATGGCTTTAGTCAGCACACGCATTCATCGACG
TCGCAGTCGAGCTCTCGCCCGCCTTGAACCACTTGCAGCCCGATGGCAGGAAACCCTAAGCAAACACAAGGAACGGTTGC
GACTCGACTATCAGCCAGGCAGCCAACTCGAAGGTGAGGAAGCGGAAGAACCTTGGAGGTTGGCGATCGAAACGCAACTC
CTCGGCCAGCGCAGTGAGGAGGAACGGTTAGGAAGTTGCCGGATCGGTCCTCACCGCGACGAGGTGAGATTGCTACTCAA
CGATTCAGAAGCGCGCCGCTTTGGCTCAGCAGGACAACAACGAACCGTGGTGCTGGCGCTCAAGCTTGCCGAACTAGAAC
TCGTAGGCGAACTCTGCGGTGAACCCCCTCTGCTGTTATTGGATGACGTGCTTGCGGAACTGGATCCCGGCAGACAGTTG
CTGCTGCTCGAGGCTGTAGGAGAGAAACATCAGTGCCTCGTTAGCGCGACCCATTTGGAAGGCTTCCAGGATGAATGGCA
ACAACAATCACAGATCATCGAAGCAGGATCTTTAAATCGATCGGAAGAGGTCCGCTAA

Upstream 100 bases:

>100_bases
CGGCTGGATGCCGCTATATGAGAGCAACTCTCAAGCCCCCTCAATCAAGGTACCTTAACCTCATGCCGCTCCAAGACCGA
TTCGGCTTCAACAGCTCGAA

Downstream 100 bases:

>100_bases
GGTGGCGAGCTGATTTTTATTGCCTTGATGGAGAGAACCCTGTCTTGCTTGCATCCCAATCCAGGATGGGAAGACACCAA
TTGCCAAGACAACACAAGCT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MQGYRNYSRLQLELTENRLLVIGPNGIGKSNLLEAIELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKLELELRRQ
GGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRH
IPIQERDSLLDAFDVQMALVSTRIHRRRSRALARLEPLAARWQETLSKHKERLRLDYQPGSQLEGEEAEEPWRLAIETQL
LGQRSEEERLGSCRIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAELDPGRQL
LLLEAVGEKHQCLVSATHLEGFQDEWQQQSQIIEAGSLNRSEEVR

Sequences:

>Translated_365_residues
MQGYRNYSRLQLELTENRLLVIGPNGIGKSNLLEAIELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKLELELRRQ
GGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRH
IPIQERDSLLDAFDVQMALVSTRIHRRRSRALARLEPLAARWQETLSKHKERLRLDYQPGSQLEGEEAEEPWRLAIETQL
LGQRSEEERLGSCRIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAELDPGRQL
LLLEAVGEKHQCLVSATHLEGFQDEWQQQSQIIEAGSLNRSEEVR
>Mature_365_residues
MQGYRNYSRLQLELTENRLLVIGPNGIGKSNLLEAIELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKLELELRRQ
GGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRH
IPIQERDSLLDAFDVQMALVSTRIHRRRSRALARLEPLAARWQETLSKHKERLRLDYQPGSQLEGEEAEEPWRLAIETQL
LGQRSEEERLGSCRIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAELDPGRQL
LLLEAVGEKHQCLVSATHLEGFQDEWQQQSQIIEAGSLNRSEEVR

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=370, Percent_Identity=25.9459459459459, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_PROMM (Q7V559)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895542.1
- ProteinModelPortal:   Q7V559
- SMR:   Q7V559
- STRING:   Q7V559
- GeneID:   1728079
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1715
- NMPDR:   fig|74547.1.peg.1709
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK919804
- BioCyc:   PMAR74547:PMT1715-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42064; Mature: 42064

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGYRNYSRLQLELTENRLLVIGPNGIGKSNLLEAIELLGSLRSHRASSDQDLIHWEEQR
CCCCCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ALLRAIADDTEKLELELRRQGGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPA
HHHHHHHCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCHHHHHCCHHHEEEEECCCHH
LRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAFDVQMALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
STRIHRRRSRALARLEPLAARWQETLSKHKERLRLDYQPGSQLEGEEAEEPWRLAIETQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHH
LGQRSEEERLGSCRIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCG
HCCCCCHHHCCCCCCCCCCCHHEEEECCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHC
EPPLLLLDDVLAELDPGRQLLLLEAVGEKHQCLVSATHLEGFQDEWQQQSQIIEAGSLNR
CCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEHHHHCCHHHHHHHHHHHHHHCCCCC
SEEVR
CCCCC
>Mature Secondary Structure
MQGYRNYSRLQLELTENRLLVIGPNGIGKSNLLEAIELLGSLRSHRASSDQDLIHWEEQR
CCCCCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ALLRAIADDTEKLELELRRQGGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPA
HHHHHHHCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCHHHHHCCHHHEEEEECCCHH
LRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAFDVQMALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
STRIHRRRSRALARLEPLAARWQETLSKHKERLRLDYQPGSQLEGEEAEEPWRLAIETQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHH
LGQRSEEERLGSCRIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCG
HCCCCCHHHCCCCCCCCCCCHHEEEECCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHC
EPPLLLLDDVLAELDPGRQLLLLEAVGEKHQCLVSATHLEGFQDEWQQQSQIIEAGSLNR
CCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEHHHHCCHHHHHHHHHHHHHHCCCCC
SEEVR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642