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The map label for this gene is purB [H]

Identifier: 33863751

GI number: 33863751

Start: 1566257

End: 1567552

Strand: Direct

Name: purB [H]

Synonym: PMT1484

Alternate gene names: 33863751

Gene position: 1566257-1567552 (Clockwise)

Preceding gene: 33863750

Following gene: 33863752

Centisome position: 64.97

GC content: 55.94

Gene sequence:

>1296_bases
TTGATCGAGCGCTACACACTGCCCGAAATGGGCGAGATTTGGACCGACAGGGCCAAGTATCAAAGCTGGCTAGATGTAGA
GATCGCCGCTTGTGAGGCCAACTGTCAACTGGGGAAGATCCCAGCGGCTGAAATGCAGCAGATTCGTGAACGCGCAACCT
TCGAGCCACAGCGCATCTTGGAGATTGAGGCAGAGGTTCGCCACGACGTCATCGCCTTCCTGACCAACGTTAATGAACAC
GTAGGGGATGCCGGCCGCTACATCCACGTCGGCATGACCAGCAGCGATGTGCTGGATACGGGTCTGGCCCTGCAGTTAAA
AAACTCTGTGGCATTGCTAAAACAAGAACTGGGCAGCCTTCAAGAAGCGATCCGCAGCTTGGCAGTGGAGCACAAGGGCA
CAGTCATGATCGGCCGCTCCCATGCCATCCATGGCGAACCCATCACCTTCGGTTTCAAACTGGCCGGTTGGCTAGCAGAA
ACAATGCGCAATGCCGAGCGACTGGAAAGGCTGGAGAGGGATGTGGCTGTAGGCCAGATCAGTGGCGCCATGGGCACCTA
CGCCAATACCGATCCAAAGGTTGAGCAGCTCACATGCGAGCGCCTTTGCCTCATCCCAGACACCGCTAGTACCCAGGTCA
TCTCTCGCGATCGTCATGCGGACTATGTACAGACCCTGGCATTAGTGGGGGCGTCTCTAGATCGATTCGCGACAGAGATC
CGCAACTTGCAGCGAACCGATGTACTGGAAGTGGAGGAGAGCTTTGCTAAAGGACAAAAGGGAAGTTCGGCGATGCCACA
TAAACGCAACCCGATTCGGGCTGAGCGGATTAGTGGTCTTGCAAGGGTCCTACGCAGCTATGTCGTCGCAGCACTCGAAA
ACGTGGCCCTCTGGCATGAGCGTGATATCAGTCACAGCTCCACTGAGCGAATGATGCTGCCGGATTGCTCCGTCACACTC
CACTTCATGTTGCGAGAGATGACCCAAGTCGTGCAGGGCCTTGGTATCTACCCAGCAAACATGCGTCGCAACATGAATAT
CTATGGCGGCGTGGTGTTCAGTCAGCGGGTGCTATTGGCGCTTGTTGAGAACGGCATGAGCAGAGAAGATGCCTACAGTG
TTGTCCAGCGCAACGCCCATGCTGCGTGGAATACCGAAGGGGGTAACTTCCGCGCCAATCTTGAGGCCGATCCTGAAGTA
TCGACCCTTCTCAATGCCAAGGCGCTAGCCGAATGCTTCAGCACAGAGCTACACCAAGCCAACCTGGACGTGATCTGGCA
ACGGCTCGGACTCTGA

Upstream 100 bases:

>100_bases
CTGGGGGCAACATGTTCATTGGGAAGTTGAACACCGCCGACTCAAGTCAGCCTGAATCCAAAGACTTAAGTTCAAGAATC
AACCGCCCATCTGTGAGCCC

Downstream 100 bases:

>100_bases
ATCCGATGTTCTGGACGTCTAAACCGATTGGAATGATTTGAGTCGTTTAAGTCTCGCCAGACTTTTTACCATCATTGAAT
GGGTTCTAACGCGCTCTCAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGEIWTDRAKYQSWLDVEIAACEANCQLGKIPAAEMQQIRERATFEPQRILEIEAEVRHDVIAFLTNVNEH
VGDAGRYIHVGMTSSDVLDTGLALQLKNSVALLKQELGSLQEAIRSLAVEHKGTVMIGRSHAIHGEPITFGFKLAGWLAE
TMRNAERLERLERDVAVGQISGAMGTYANTDPKVEQLTCERLCLIPDTASTQVISRDRHADYVQTLALVGASLDRFATEI
RNLQRTDVLEVEESFAKGQKGSSAMPHKRNPIRAERISGLARVLRSYVVAALENVALWHERDISHSSTERMMLPDCSVTL
HFMLREMTQVVQGLGIYPANMRRNMNIYGGVVFSQRVLLALVENGMSREDAYSVVQRNAHAAWNTEGGNFRANLEADPEV
STLLNAKALAECFSTELHQANLDVIWQRLGL

Sequences:

>Translated_431_residues
MIERYTLPEMGEIWTDRAKYQSWLDVEIAACEANCQLGKIPAAEMQQIRERATFEPQRILEIEAEVRHDVIAFLTNVNEH
VGDAGRYIHVGMTSSDVLDTGLALQLKNSVALLKQELGSLQEAIRSLAVEHKGTVMIGRSHAIHGEPITFGFKLAGWLAE
TMRNAERLERLERDVAVGQISGAMGTYANTDPKVEQLTCERLCLIPDTASTQVISRDRHADYVQTLALVGASLDRFATEI
RNLQRTDVLEVEESFAKGQKGSSAMPHKRNPIRAERISGLARVLRSYVVAALENVALWHERDISHSSTERMMLPDCSVTL
HFMLREMTQVVQGLGIYPANMRRNMNIYGGVVFSQRVLLALVENGMSREDAYSVVQRNAHAAWNTEGGNFRANLEADPEV
STLLNAKALAECFSTELHQANLDVIWQRLGL
>Mature_431_residues
MIERYTLPEMGEIWTDRAKYQSWLDVEIAACEANCQLGKIPAAEMQQIRERATFEPQRILEIEAEVRHDVIAFLTNVNEH
VGDAGRYIHVGMTSSDVLDTGLALQLKNSVALLKQELGSLQEAIRSLAVEHKGTVMIGRSHAIHGEPITFGFKLAGWLAE
TMRNAERLERLERDVAVGQISGAMGTYANTDPKVEQLTCERLCLIPDTASTQVISRDRHADYVQTLALVGASLDRFATEI
RNLQRTDVLEVEESFAKGQKGSSAMPHKRNPIRAERISGLARVLRSYVVAALENVALWHERDISHSSTERMMLPDCSVTL
HFMLREMTQVVQGLGIYPANMRRNMNIYGGVVFSQRVLLALVENGMSREDAYSVVQRNAHAAWNTEGGNFRANLEADPEV
STLLNAKALAECFSTELHQANLDVIWQRLGL

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=409, Percent_Identity=29.0953545232274, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI183227688, Length=386, Percent_Identity=29.2746113989637, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1790398, Length=260, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787376, Length=277, Percent_Identity=27.4368231046931, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=406, Percent_Identity=26.3546798029557, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=329, Percent_Identity=27.6595744680851, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=409, Percent_Identity=28.8508557457213, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24647570, Length=420, Percent_Identity=25.952380952381, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48151; Mature: 48151

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGEIWTDRAKYQSWLDVEIAACEANCQLGKIPAAEMQQIRERATFEPQRIL
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHH
EIEAEVRHDVIAFLTNVNEHVGDAGRYIHVGMTSSDVLDTGLALQLKNSVALLKQELGSL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
QEAIRSLAVEHKGTVMIGRSHAIHGEPITFGFKLAGWLAETMRNAERLERLERDVAVGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGTYANTDPKVEQLTCERLCLIPDTASTQVISRDRHADYVQTLALVGASLDRFATEI
CCCCCCCCCCCCCHHHHHHHHEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEESFAKGQKGSSAMPHKRNPIRAERISGLARVLRSYVVAALENVALWHE
HHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSTERMMLPDCSVTLHFMLREMTQVVQGLGIYPANMRRNMNIYGGVVFSQRVLLA
CCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
LVENGMSREDAYSVVQRNAHAAWNTEGGNFRANLEADPEVSTLLNAKALAECFSTELHQA
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
NLDVIWQRLGL
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGEIWTDRAKYQSWLDVEIAACEANCQLGKIPAAEMQQIRERATFEPQRIL
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHH
EIEAEVRHDVIAFLTNVNEHVGDAGRYIHVGMTSSDVLDTGLALQLKNSVALLKQELGSL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
QEAIRSLAVEHKGTVMIGRSHAIHGEPITFGFKLAGWLAETMRNAERLERLERDVAVGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGTYANTDPKVEQLTCERLCLIPDTASTQVISRDRHADYVQTLALVGASLDRFATEI
CCCCCCCCCCCCCHHHHHHHHEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEESFAKGQKGSSAMPHKRNPIRAERISGLARVLRSYVVAALENVALWHE
HHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSTERMMLPDCSVTLHFMLREMTQVVQGLGIYPANMRRNMNIYGGVVFSQRVLLA
CCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
LVENGMSREDAYSVVQRNAHAAWNTEGGNFRANLEADPEVSTLLNAKALAECFSTELHQA
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
NLDVIWQRLGL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]