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The map label for this gene is tyrS

Identifier: 33863670

GI number: 33863670

Start: 1489398

End: 1490624

Strand: Reverse

Name: tyrS

Synonym: PMT1403

Alternate gene names: 33863670

Gene position: 1490624-1489398 (Counterclockwise)

Preceding gene: 33863671

Following gene: 33863669

Centisome position: 61.83

GC content: 53.95

Gene sequence:

>1227_bases
ATGCCGGCCTGGTTGCAGCGGGGCATGGTTGACTTGTTCCCTTCAGGCCAATGGGGGGATGCTGATCAGCAACTTGCGAC
TCGTCTTGATGAGGCTCGAGAACAAAACCGACCCCTGAGGGTCAAGCTTGGTATTGATCCCACTGGTCGTGATATCCATC
TGGGACACAGCATCCTGTTTCGCAAACTGAGAGCTTTTCAGGATGCGGGGCATACAGCAGTGTTGATTATTGGCGATTTC
ACTGCACGAATCGGTGACCCAACCGGCAAGAGCAGCACCAGAGTGCAACTCACCTCTGAGCAGATTGAGGCCAATGCAAC
GACCTATCTTGCACAGCTGGGTCAGGGGCAGTCAGCGGAAAAAGCGTTGCTCGATTTCACAACCCCTGGGCGCCTGGAAG
TGCGACGTAACACTGAGTGGTTGGCTGACTTGGATTTGCCTGAGGTGATTGGTTTGTTGGGCACTGCCACGGTTGGGCAA
ATGCTGGCTAAAGAAGACTTTGGCAATCGTTATGGCTCAGGTGTTCCGATAGCTCTGCATGAGTTTCTTTATCCCCTCTT
GCAGGGTTACGACTCGGTAGCGGTGCAGGCAGATGTTGAACTTGGGGGGACTGATCAGAAATTCAATGTCGCCATGGGGC
GGGATCTTCAGCGTCATTTCGATCAGCGCCCCCAGTTTGGGCTCCTACTCCCAATTCTGGCTGGGCTGGATGGGGTGCAG
AAGATGAGCAAGAGCCTCTCTAACACTGTGGGACTGAATGAGGACCCACTTTCCATGTATTCCAAGTTGGAGAAGGTTCC
TGATGCCCTTGTAAGCAGTTACGTGGTTTTGCTTACCGATCTCGACCCAGAAGCTTTACCTGTCAATCCGCGTGAACGGC
AGAAGGCCATGGCCATTGCGGTCACGGCCAGCCGTCATGGCATCGCGGCGGCTGAAGCCGCCCAGAATGATGCAGCTAGA
TTGGTTTCCGGCTCTCAAGATGACGCTGCATCTGTGCCAGAAGCGTTTCTTTCGGATGTGAATTTTCCGGCCAAGGCCTT
TTATCTGCTCAGTGCGATTGGCCTTTGTGCGAGCAGTAGCGAGGCTCGGCGTCAAATCAAGGGTGGTGCGGTACGCCTGG
ATGGCGAAAAAATTACCGATCCCAATTTGGAATTCACTGACTCCAGCCTGCTGATGGGCAAGGTGCTGCAAGTGGGCAAG
AAAACCTTTCGTCGTTTGACGCGTTGA

Upstream 100 bases:

>100_bases
TTGAGCAGGACTCTCGATGAAAGTGATGACTGGCTGACTCGCTACCCGTACGATCCGCTCAGCTAGAGATCCTCTAACGA
TGCCGGAGACGCCGATCACC

Downstream 100 bases:

>100_bases
GTAAAGGACCAACACCCGCTGTGATGACCTCGCCATTGACTCCTGCCGATCAACTGATCATTGCTCTTGATGGCATGGAG
CGGGCTGAGGCCCTTGCATT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPAWLQRGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILFRKLRAFQDAGHTAVLIIGDF
TARIGDPTGKSSTRVQLTSEQIEANATTYLAQLGQGQSAEKALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQ
MLAKEDFGNRYGSGVPIALHEFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
KMSKSLSNTVGLNEDPLSMYSKLEKVPDALVSSYVVLLTDLDPEALPVNPRERQKAMAIAVTASRHGIAAAEAAQNDAAR
LVSGSQDDAASVPEAFLSDVNFPAKAFYLLSAIGLCASSSEARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGK
KTFRRLTR

Sequences:

>Translated_408_residues
MPAWLQRGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILFRKLRAFQDAGHTAVLIIGDF
TARIGDPTGKSSTRVQLTSEQIEANATTYLAQLGQGQSAEKALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQ
MLAKEDFGNRYGSGVPIALHEFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
KMSKSLSNTVGLNEDPLSMYSKLEKVPDALVSSYVVLLTDLDPEALPVNPRERQKAMAIAVTASRHGIAAAEAAQNDAAR
LVSGSQDDAASVPEAFLSDVNFPAKAFYLLSAIGLCASSSEARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGK
KTFRRLTR
>Mature_407_residues
PAWLQRGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILFRKLRAFQDAGHTAVLIIGDFT
ARIGDPTGKSSTRVQLTSEQIEANATTYLAQLGQGQSAEKALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQM
LAKEDFGNRYGSGVPIALHEFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQK
MSKSLSNTVGLNEDPLSMYSKLEKVPDALVSSYVVLLTDLDPEALPVNPRERQKAMAIAVTASRHGIAAAEAAQNDAARL
VSGSQDDAASVPEAFLSDVNFPAKAFYLLSAIGLCASSSEARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGKK
TFRRLTR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=27.7915632754342, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1787925, Length=412, Percent_Identity=28.6407766990291, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=384, Percent_Identity=23.6979166666667, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325160, Length=412, Percent_Identity=25.2427184466019, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=25.8706467661692, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_PROMM (Q7TUT0)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895230.1
- ProteinModelPortal:   Q7TUT0
- SMR:   Q7TUT0
- STRING:   Q7TUT0
- GeneID:   1727678
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1403
- NMPDR:   fig|74547.1.peg.1397
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- ProtClustDB:   PRK05912
- BioCyc:   PMAR74547:PMT1403-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44323; Mature: 44192

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAWLQRGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILF
CCCHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHH
RKLRAFQDAGHTAVLIIGDFTARIGDPTGKSSTRVQLTSEQIEANATTYLAQLGQGQSAE
HHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHH
KALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQMLAKEDFGNRYGSGVPIALH
HHHHCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
HHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
KMSKSLSNTVGLNEDPLSMYSKLEKVPDALVSSYVVLLTDLDPEALPVNPRERQKAMAIA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHCEEEEE
VTASRHGIAAAEAAQNDAARLVSGSQDDAASVPEAFLSDVNFPAKAFYLLSAIGLCASSS
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
EARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGKKTFRRLTR
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAWLQRGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILF
CCHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHH
RKLRAFQDAGHTAVLIIGDFTARIGDPTGKSSTRVQLTSEQIEANATTYLAQLGQGQSAE
HHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHH
KALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQMLAKEDFGNRYGSGVPIALH
HHHHCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
HHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
KMSKSLSNTVGLNEDPLSMYSKLEKVPDALVSSYVVLLTDLDPEALPVNPRERQKAMAIA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHCEEEEE
VTASRHGIAAAEAAQNDAARLVSGSQDDAASVPEAFLSDVNFPAKAFYLLSAIGLCASSS
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
EARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGKKTFRRLTR
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642