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The map label for this gene is pyrF

Identifier: 33863669

GI number: 33863669

Start: 1488637

End: 1489374

Strand: Reverse

Name: pyrF

Synonym: PMT1402

Alternate gene names: 33863669

Gene position: 1489374-1488637 (Counterclockwise)

Preceding gene: 33863670

Following gene: 33863663

Centisome position: 61.78

GC content: 57.59

Gene sequence:

>738_bases
ATGACCTCGCCATTGACTCCTGCCGATCAACTGATCATTGCTCTTGATGGCATGGAGCGGGCTGAGGCCCTTGCATTCAT
CTCAAAGTTGCCAGAGGTCAGTTGGGTCAAGGTGGGACTGGAACTGTTTGTTAGTGCGGGGCCAGAGGTTTTGGCTGATC
TACGGGAGCAAGGGTTGCAGATTTTTCTTGATCTAAAGTTTCACGATATCCCTGCCACCATTGCAGGAGCTTGTCGCCGA
GCGGCGAGCTTTGGCGCTGAGTTGATCACGGTGCATGCCTGTGCTGGCCGTGCTGCTCTTGCTGAAGCTCAGGCTGCTGC
TTGCGAGGGGGCTGCGGAGGTTGGCTTGTCAGCACCCCGGCTGTTGGCCGTTACTGTGCTCACCAGTTGGGATCAGAAGC
GTCTAGCGAATGAGCTGTGCATTCCTCAGTCTCTTCAGGCCAGGGTGGAGTGGTTGGCTGAGTTGGCAGCGGAGTCAGGG
TTGGGCGGCTGCGTGTGTTCTCCCTGGGAGGTCAGCGGCTTACGCCGACTTCATCCCTCGCCATTTGAGTTGGTGACTCC
TGGTATTCGCCCCTCGGGGGCTGAATTGGCGGATCAGGTCAGGGTCATGAGTCCTGCAGCGGCGTTGAATGCAGGAGCTT
CACGTTTGGTGATTGGCAGGCCGATTACCCGTGCTGTTGATCCGGCAGAAGCGTTTGCACGCTGCTGCCTTGAGATTGAA
CAGCGTCAGCTGGACTGA

Upstream 100 bases:

>100_bases
AATTCACTGACTCCAGCCTGCTGATGGGCAAGGTGCTGCAAGTGGGCAAGAAAACCTTTCGTCGTTTGACGCGTTGAGTA
AAGGACCAACACCCGCTGTG

Downstream 100 bases:

>100_bases
CCGAGGCGAGGTTCGGTGCCCGCCAGCAAGCGTTGCAGATTGGAGCGGTGTCGCCACACCACCATTGCCATGGCGGCAAA
GGCCACCGCCAGGTAAGCCG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLQIFLDLKFHDIPATIAGACRR
AASFGAELITVHACAGRAALAEAQAAACEGAAEVGLSAPRLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESG
LGGCVCSPWEVSGLRRLHPSPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCLEIE
QRQLD

Sequences:

>Translated_245_residues
MTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLQIFLDLKFHDIPATIAGACRR
AASFGAELITVHACAGRAALAEAQAAACEGAAEVGLSAPRLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESG
LGGCVCSPWEVSGLRRLHPSPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCLEIE
QRQLD
>Mature_244_residues
TSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLQIFLDLKFHDIPATIAGACRRA
ASFGAELITVHACAGRAALAEAQAAACEGAAEVGLSAPRLLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESGL
GGCVCSPWEVSGLRRLHPSPFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCLEIEQ
RQLD

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=223, Percent_Identity=44.3946188340807, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PROMM (Q7V5Y2)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895229.1
- ProteinModelPortal:   Q7V5Y2
- STRING:   Q7V5Y2
- GeneID:   1727479
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1402
- NMPDR:   fig|74547.1.peg.1396
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   PMAR74547:PMT1402-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25910; Mature: 25779

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 66-66 BINDING 16-16 BINDING 37-37 BINDING 128-128 BINDING 190-190 BINDING 199-199 BINDING 219-219 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLQ
CCCCCCCHHHEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCE
IFLDLKFHDIPATIAGACRRAASFGAELITVHACAGRAALAEAQAAACEGAAEVGLSAPR
EEEEEEECCCCHHHHHHHHHHHHCCHHHEEEEHHCCHHHHHHHHHHHHCCHHHHCCCCCH
LLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESGLGGCVCSPWEVSGLRRLHPS
HHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCC
PFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCLEIE
CHHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHH
QRQLD
HHCCC
>Mature Secondary Structure 
TSPLTPADQLIIALDGMERAEALAFISKLPEVSWVKVGLELFVSAGPEVLADLREQGLQ
CCCCCCHHHEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCE
IFLDLKFHDIPATIAGACRRAASFGAELITVHACAGRAALAEAQAAACEGAAEVGLSAPR
EEEEEEECCCCHHHHHHHHHHHHCCHHHEEEEHHCCHHHHHHHHHHHHCCHHHHCCCCCH
LLAVTVLTSWDQKRLANELCIPQSLQARVEWLAELAAESGLGGCVCSPWEVSGLRRLHPS
HHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCC
PFELVTPGIRPSGAELADQVRVMSPAAALNAGASRLVIGRPITRAVDPAEAFARCCLEIE
CHHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHH
QRQLD
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642