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The map label for this gene is ribE [H]

Identifier: 33863613

GI number: 33863613

Start: 1431042

End: 1431704

Strand: Reverse

Name: ribE [H]

Synonym: PMT1346

Alternate gene names: 33863613

Gene position: 1431704-1431042 (Counterclockwise)

Preceding gene: 33863616

Following gene: 33863612

Centisome position: 59.39

GC content: 57.01

Gene sequence:

>663_bases
ATGTTTACGGGGCTAGTTCAGGCGGTGGGACGGGTGCAGCGTCGAGCGACGGGCTTGTTGGTGCAAGGCTGTTCCCCATT
AGCACCTCTGGCAGAGGGAGACAGTGTGGCTGTTGATGGGGTCTGCCTGACGGTGGCTGAATTGATCGCGGATGGTTTTC
GTGCTGATGTCAGTGAGGAGACGCTTTGTCGAACCAGCCTTGGGGCCAAGGCGGATCGCGGTGGGGTTGTGAACCTTGAA
CCAGCCCTGCGCTTCTGCGACCGTCTCGGAGGCCATCTGGTCAGTGGCCATGTTGATGGATCTGGAGAGGTGGTGGCTCT
GGATGCTCGCTCAACGTCCTGGTATCTGGAGGTCTGCTGGCAAGATCCTGCCTTCGGACGTTATGTCTGCGAAAAAGCCA
GCATTGCTTTAGATGGCATCAGTCTCACTGTTGCGGGATGCGTTGAAGACGGCAGCCGATTCTGGATAGCGGTGATAGCT
CATACCTGGACGAGCACGTCTTTGCAACATTTGGAGGTTGGAGCTGTGGTGAATCTGGAGGCCGATCTGTTGGCCAAATA
CACCGAGCGTCTTTTGGCAGGGGCGCCTGATTTACCAATGGCAAGCAACCGGAACACTCAAGAGATCTCAACGGAGTGGC
TTGCCAGCCATGGGTGGAGCTGA

Upstream 100 bases:

>100_bases
ACGCGTCGAGTGCGAGACCGGCAACGCTCATCTCGACCACTGACCCTCCATCCCCCTTTTGTCCGATTATGGCCAGTGAT
GTTCCCACATACGGCCTGCC

Downstream 100 bases:

>100_bases
GTCGCTACGTCTTTTGCACAGGGATCTTACAAAGCATGGCCGGATCAGGAGACCCTCAATAGCGTTCAGATGCTGTTCAT
TTTGAACATTTCATGACCGT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MFTGLVQAVGRVQRRATGLLVQGCSPLAPLAEGDSVAVDGVCLTVAELIADGFRADVSEETLCRTSLGAKADRGGVVNLE
PALRFCDRLGGHLVSGHVDGSGEVVALDARSTSWYLEVCWQDPAFGRYVCEKASIALDGISLTVAGCVEDGSRFWIAVIA
HTWTSTSLQHLEVGAVVNLEADLLAKYTERLLAGAPDLPMASNRNTQEISTEWLASHGWS

Sequences:

>Translated_220_residues
MFTGLVQAVGRVQRRATGLLVQGCSPLAPLAEGDSVAVDGVCLTVAELIADGFRADVSEETLCRTSLGAKADRGGVVNLE
PALRFCDRLGGHLVSGHVDGSGEVVALDARSTSWYLEVCWQDPAFGRYVCEKASIALDGISLTVAGCVEDGSRFWIAVIA
HTWTSTSLQHLEVGAVVNLEADLLAKYTERLLAGAPDLPMASNRNTQEISTEWLASHGWS
>Mature_220_residues
MFTGLVQAVGRVQRRATGLLVQGCSPLAPLAEGDSVAVDGVCLTVAELIADGFRADVSEETLCRTSLGAKADRGGVVNLE
PALRFCDRLGGHLVSGHVDGSGEVVALDARSTSWYLEVCWQDPAFGRYVCEKASIALDGISLTVAGCVEDGSRFWIAVIA
HTWTSTSLQHLEVGAVVNLEADLLAKYTERLLAGAPDLPMASNRNTQEISTEWLASHGWS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=203, Percent_Identity=34.4827586206897, Blast_Score=94, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319733, Length=206, Percent_Identity=33.9805825242718, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23357; Mature: 23357

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGLVQAVGRVQRRATGLLVQGCSPLAPLAEGDSVAVDGVCLTVAELIADGFRADVSEE
CCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCHH
TLCRTSLGAKADRGGVVNLEPALRFCDRLGGHLVSGHVDGSGEVVALDARSTSWYLEVCW
HHHHHHCCCCCCCCCEEECHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCEEEEEEEE
QDPAFGRYVCEKASIALDGISLTVAGCVEDGSRFWIAVIAHTWTSTSLQHLEVGAVVNLE
CCCCCHHHHHHHCCEEECCEEEEEEEEECCCCEEEEEEEEECCCCCCHHEEEECEEEECH
ADLLAKYTERLLAGAPDLPMASNRNTQEISTEWLASHGWS
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGLVQAVGRVQRRATGLLVQGCSPLAPLAEGDSVAVDGVCLTVAELIADGFRADVSEE
CCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCHH
TLCRTSLGAKADRGGVVNLEPALRFCDRLGGHLVSGHVDGSGEVVALDARSTSWYLEVCW
HHHHHHCCCCCCCCCEEECHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCEEEEEEEE
QDPAFGRYVCEKASIALDGISLTVAGCVEDGSRFWIAVIAHTWTSTSLQHLEVGAVVNLE
CCCCCHHHHHHHCCEEECCEEEEEEEEECCCCEEEEEEEEECCCCCCHHEEEECEEEECH
ADLLAKYTERLLAGAPDLPMASNRNTQEISTEWLASHGWS
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]