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The map label for this gene is cobM [H]

Identifier: 33863592

GI number: 33863592

Start: 1409549

End: 1410304

Strand: Direct

Name: cobM [H]

Synonym: PMT1325

Alternate gene names: 33863592

Gene position: 1409549-1410304 (Clockwise)

Preceding gene: 33863591

Following gene: 33863593

Centisome position: 58.47

GC content: 54.89

Gene sequence:

>756_bases
ATGAGCAGAATCTCCATCGTTGGTGCAGGTCCAGGCGCGACTGATCTGCTAACCCTGCGTGCCGCAGAACATCTCAAGCA
GGCTGAGGTCCTTGTATGGACCGATTCACTGGTTTCACCAGATATTGCAGCATTGGCACCTGAATCCTGTGAACGAATCC
GCACCAGCTCAATGACCCTCGAGGAAGTTCTTCCGCTTCTGGTGAACAGGGCACAAGCAGGCAAGCGGGTGGTCCGTCTT
CACGACGGAGACCCCTGCCTTTATGGGGCCCTGTCTGAACAGATCTGTGGCCTTGCTGACGCAGGCATTGAAGTAGAGGT
TGTGCCGGGTTTGAGTGCTTACCAAGCGACAGCCGCAGCCCTAAATGCTGAACTCACCATCCCTGGCCTTGTCCAAACCA
TAGTTCTCAGCAGAGCAGGAGGACGCACCGGTGTTCCCGAGCGCGAAAACCTCCAACATCTGGCATCACTTGGAGCCTCT
CTATGCCTCTACCTCAGTGCACGCCATGTAGAGGAAGTACAAGCCACACTGCTGCAGCACTATTCAGCTGAAACTCCAGT
AGCTATCGGCTACAGGGTGAGCTGGCCCGATCAATGGTTAAGCGTGGTGCCTTTGAAGCAAATGGCTACGACCTCCCGTG
AACACGAGCTGATTCGCACCACCCTTTATGTCGTTAGTCCGGCCCTAGCTGCAGGACGCCAACGTTCGAAGCTGTATTCT
CCAAGCCACAAACATCTCTTTCGCCAAAAGGGCTAA

Upstream 100 bases:

>100_bases
TGAGTTTGGCGATGATGGCAGTTGGAGGTTTTGGCCTCTGGTGGCTCTATGGTCGCAAACGAAAGCTACCGGACCCTGGT
AGGCCTAGGTCATTCGCTTG

Downstream 100 bases:

>100_bases
AGAGAGTGTTTTAAAATCCAGTAGTGCGGGCGATTAGCACAGTGGTAGCGCACTTCCTTCACACGGAAGGGGTCGTGGGT
TCGAATCCCGCATCGCCCAT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTLEEVLPLLVNRAQAGKRVVRL
HDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAALNAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASLGAS
LCLYLSARHVEEVQATLLQHYSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
PSHKHLFRQKG

Sequences:

>Translated_251_residues
MSRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTLEEVLPLLVNRAQAGKRVVRL
HDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAALNAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASLGAS
LCLYLSARHVEEVQATLLQHYSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
PSHKHLFRQKG
>Mature_250_residues
SRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTLEEVLPLLVNRAQAGKRVVRLH
DGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAALNAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASLGASL
CLYLSARHVEEVQATLLQHYSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYSP
SHKHLFRQKG

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=193, Percent_Identity=34.7150259067358, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=218, Percent_Identity=28.4403669724771, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27057; Mature: 26926

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTL
CCCEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHCCHHHHHHHHHHCCCH
EEVLPLLVNRAQAGKRVVRLHDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAA
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
LNAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASLGASLCLYLSARHVEEVQATLLQH
HCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
HCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCC
PSHKHLFRQKG
CHHHHHHHCCC
>Mature Secondary Structure 
SRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTL
CCEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHCCHHHHHHHHHHCCCH
EEVLPLLVNRAQAGKRVVRLHDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAA
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
LNAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASLGASLCLYLSARHVEEVQATLLQH
HCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
HCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCC
PSHKHLFRQKG
CHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]