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The map label for this gene is rluE [H]

Identifier: 33863440

GI number: 33863440

Start: 1262582

End: 1263229

Strand: Direct

Name: rluE [H]

Synonym: PMT1170

Alternate gene names: 33863440

Gene position: 1262582-1263229 (Clockwise)

Preceding gene: 33863438

Following gene: 33863445

Centisome position: 52.37

GC content: 55.56

Gene sequence:

>648_bases
CTGACCACCCAAGCAGACGACTTCACGACCCTTCTGTTGAACAAGCCCTACGGCTTCCTAAGCCAGTTCACCCCAGAACC
AGGCAGCCAGTGGAGGTGCTTAGCAGACTTGATCCCCATACCCAGGGTGTATGCGGCAGGCCGCCTAGATGCTGACAGCG
AAGGCCTCTTGGTTCTCACTTCCCATGGTCGCCTGCAGCATCGACTCACCAATCCCCATTTGAGCCACTGGCGGGTTTAT
TGGGTGCAAGTAGAGGGTTCACCTAGTGAAAGTCAACTACAAAAACTTGCCTCAGGAGTGATCATCAAGGGAAAGAGAAC
CCGTCCTGCCCTAGCGAGCTGGCTGAAGGGAAACGTTGCTCCGATCGTGAGAGAGCGTGAACCACCCATCCGTGTGCGGC
AAAGCATCCCCACTAGCTGGCTTGAATTGAAGTTAAAAGAAGGGAGAAACAGACAGGTTCGACGGATGACAGCAGCCGTA
GGGCTGCCAACCCTTCGCTTGATCCGTCATCGCATCGAGCTTATGGATGGTGAGCCACCCCTCAGCATTGAAGGGATGGA
TCCTGGCCAATGGAGGCAGGTCTCATCAAGAGAGACCAACCTCCTGCTGTCCCTCCTCAATCATCCGGCAGCTGACCCAC
AAGCGTGA

Upstream 100 bases:

>100_bases
GGCTTAAACGATGACTCATTTCTATTGAAGCAATCAAGCGCTGACGATCCCTACAACGATTTATCTATGCTGATACTGCT
CGCGTCTACTGACTGATCAG

Downstream 100 bases:

>100_bases
CGGCGCGAAATTCAATGTCTTCAACCACCTGCCAGGGTTGATCAGAACGATCGGCATAGCTCACCTTCTCAAAGCCCAAA
GCACGAAAATCCTGCAGGAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTTQADDFTTLLLNKPYGFLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTSHGRLQHRLTNPHLSHWRVY
WVQVEGSPSESQLQKLASGVIIKGKRTRPALASWLKGNVAPIVREREPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAV
GLPTLRLIRHRIELMDGEPPLSIEGMDPGQWRQVSSRETNLLLSLLNHPAADPQA

Sequences:

>Translated_215_residues
MTTQADDFTTLLLNKPYGFLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTSHGRLQHRLTNPHLSHWRVY
WVQVEGSPSESQLQKLASGVIIKGKRTRPALASWLKGNVAPIVREREPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAV
GLPTLRLIRHRIELMDGEPPLSIEGMDPGQWRQVSSRETNLLLSLLNHPAADPQA
>Mature_214_residues
TTQADDFTTLLLNKPYGFLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTSHGRLQHRLTNPHLSHWRVYW
VQVEGSPSESQLQKLASGVIIKGKRTRPALASWLKGNVAPIVREREPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAVG
LPTLRLIRHRIELMDGEPPLSIEGMDPGQWRQVSSRETNLLLSLLNHPAADPQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=50.5376344086022, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1790453, Length=209, Percent_Identity=28.7081339712919, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788510, Length=191, Percent_Identity=30.3664921465969, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787524, Length=165, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24251; Mature: 24120

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQADDFTTLLLNKPYGFLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLT
CCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCHHHHHCCCCHHHCCCCCCCCCCCEEEEE
SHGRLQHRLTNPHLSHWRVYWVQVEGSPSESQLQKLASGVIIKGKRTRPALASWLKGNVA
CCCCEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCEEEECCCCCHHHHHHHCCCCC
PIVREREPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRIELMDGEPP
EEECCCCCCHHHHHCCCCHHEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
LSIEGMDPGQWRQVSSRETNLLLSLLNHPAADPQA
EEECCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTQADDFTTLLLNKPYGFLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLT
CCCCCCEEEEEEECCCHHHHHCCCCCCCCCCHHHHHCCCCHHHCCCCCCCCCCCEEEEE
SHGRLQHRLTNPHLSHWRVYWVQVEGSPSESQLQKLASGVIIKGKRTRPALASWLKGNVA
CCCCEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCEEEECCCCCHHHHHHHCCCCC
PIVREREPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRIELMDGEPP
EEECCCCCCHHHHHCCCCHHEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
LSIEGMDPGQWRQVSSRETNLLLSLLNHPAADPQA
EEECCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]