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The map label for this gene is gldA [C]

Identifier: 33863333

GI number: 33863333

Start: 1146365

End: 1147525

Strand: Direct

Name: gldA [C]

Synonym: PMT1062

Alternate gene names: 33863333

Gene position: 1146365-1147525 (Clockwise)

Preceding gene: 33863332

Following gene: 33863334

Centisome position: 47.55

GC content: 54.09

Gene sequence:

>1161_bases
ATGAACAAAAACAATTTGCCTGAGGAGGTGTCTCACTGCGCTCACTCCATAGCCCCCGAAAAGGTTGTTAGGGGAGAAAG
GGCGTGGGAAGAAAGCCATCAACTCATTGCCTCGATTTGCCGCTCACCTTTACTGCTTGGACGCAGCATCGCCACGGCTG
AGTTACGCCAGGGATTACTCAACAACCTCAGGGACCTTGGACTCAACACAGTTTCTGCTGAGCTCAAGCACGATTGCTGC
GAGATGGATCTCAAGCGGATCAATGCTCTTGCCTTGTACAACTCCTGCGACGGTGTGATTGCCGCAGGTGGGGGCAAGGT
GCTAGATGCCGGGAAGCTTTTGGCCCATCGGCTAAGCATCCCATGCATCACAGTTCCACTGAGTGCAGCCACCTGTGCCG
GTTGGACCGCCCTCGCCAATATCTATTCACCAACAGGTGCTTTTCAACGCGATCAAGTTCTGGCTCGATGTCCACATCTG
ATGATCTTTGATCATGGTCTCGTGCGTCAGGCTCCTCCCAGGACTCTCGCAAGCGGCATCGCAGATGCAATCGCCAAATG
GTACGAAGCGTCTGTCAGCAATGGCTCCAGCAACGATGGCTTAATTCAACAAGCTGTGCAGATGGCAAGGGTCTTGCGCG
ATCAGCTGCTGCTTGATGGGCCTGAAGCGCTGAAAGATCAAAACAGCCTGGCCTGGATCAGGGTTGCCGAAGCCTGCGCA
CTAACTGCAGGGCTGATCGGAGGGATTGGCGGTTCTCGCTGCCGTACTGCCGCTGCCCACGCGGTACACAACGCACTCAC
GCAATTAAAAGATTGCCATGAGGTCCTTCATGGAGAAAAGGTGGGTTACGGGATTCTCGTACAGTTGCGTCTTGAGGAGA
TTATTGGAGGCCATCAACTGGCCGGACAAGCAAGGCGACAGCTGATTCCATTCCTCAAAGGACTTGATCTGCCTGTAAAT
CTTGAGGATCTGGGCCTATCGAATTTGAGTCTTCACGAGCTGCATGAGGTCTGCCAATTCGCCTGTCAAAAAGGCTCTGA
CCTATACCAACTGCCGTTTCCCGTCAATTCCAACGCCCTACTCGAAGCACTCCTCGGCGCTAGCGACAATGGCCCGGTCC
CAGTCGAATCCACAGTCACCAAGCGAATAGCAGCCTCTTGA

Upstream 100 bases:

>100_bases
GATGTGGATGACAACAAGCAAATCGTTGTACGCCATGTCAGCAAAACTACTGCCACACCGGAACTCGCTGGAGCAGGAGT
CTGACACCTCTCCTACATCA

Downstream 100 bases:

>100_bases
ACCTCAAAACTGGAATGGCAGCCCAAACAATCGCCTCGTCAGAGACCCTTTTACGTCAGCTCACACCACAACTTCTGGAT
CCATTAGCCCGTGACCTGGC

Product: glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNKNNLPEEVSHCAHSIAPEKVVRGERAWEESHQLIASICRSPLLLGRSIATAELRQGLLNNLRDLGLNTVSAELKHDCC
EMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLSIPCITVPLSAATCAGWTALANIYSPTGAFQRDQVLARCPHL
MIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMARVLRDQLLLDGPEALKDQNSLAWIRVAEACA
LTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQLRLEEIIGGHQLAGQARRQLIPFLKGLDLPVN
LEDLGLSNLSLHELHEVCQFACQKGSDLYQLPFPVNSNALLEALLGASDNGPVPVESTVTKRIAAS

Sequences:

>Translated_386_residues
MNKNNLPEEVSHCAHSIAPEKVVRGERAWEESHQLIASICRSPLLLGRSIATAELRQGLLNNLRDLGLNTVSAELKHDCC
EMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLSIPCITVPLSAATCAGWTALANIYSPTGAFQRDQVLARCPHL
MIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMARVLRDQLLLDGPEALKDQNSLAWIRVAEACA
LTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQLRLEEIIGGHQLAGQARRQLIPFLKGLDLPVN
LEDLGLSNLSLHELHEVCQFACQKGSDLYQLPFPVNSNALLEALLGASDNGPVPVESTVTKRIAAS
>Mature_386_residues
MNKNNLPEEVSHCAHSIAPEKVVRGERAWEESHQLIASICRSPLLLGRSIATAELRQGLLNNLRDLGLNTVSAELKHDCC
EMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLSIPCITVPLSAATCAGWTALANIYSPTGAFQRDQVLARCPHL
MIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMARVLRDQLLLDGPEALKDQNSLAWIRVAEACA
LTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQLRLEEIIGGHQLAGQARRQLIPFLKGLDLPVN
LEDLGLSNLSLHELHEVCQFACQKGSDLYQLPFPVNSNALLEALLGASDNGPVPVESTVTKRIAAS

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=363, Percent_Identity=29.7520661157025, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786815, Length=298, Percent_Identity=32.5503355704698, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 41292; Mature: 41292

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNNLPEEVSHCAHSIAPEKVVRGERAWEESHQLIASICRSPLLLGRSIATAELRQGLL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHH
NNLRDLGLNTVSAELKHDCCEMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLSI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHCC
PCITVPLSAATCAGWTALANIYSPTGAFQRDQVLARCPHLMIFDHGLVRQAPPRTLASGI
CEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHCCCCHHHHHHHH
ADAIAKWYEASVSNGSSNDGLIQQAVQMARVLRDQLLLDGPEALKDQNSLAWIRVAEACA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHH
LTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQLRLEEIIGGHQL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEHHHHHCCHHH
AGQARRQLIPFLKGLDLPVNLEDLGLSNLSLHELHEVCQFACQKGSDLYQLPFPVNSNAL
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHH
LEALLGASDNGPVPVESTVTKRIAAS
HHHHHCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNKNNLPEEVSHCAHSIAPEKVVRGERAWEESHQLIASICRSPLLLGRSIATAELRQGLL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHH
NNLRDLGLNTVSAELKHDCCEMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLSI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHCC
PCITVPLSAATCAGWTALANIYSPTGAFQRDQVLARCPHLMIFDHGLVRQAPPRTLASGI
CEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHCCCCHHHHHHHH
ADAIAKWYEASVSNGSSNDGLIQQAVQMARVLRDQLLLDGPEALKDQNSLAWIRVAEACA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHH
LTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQLRLEEIIGGHQL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEHHHHHCCHHH
AGQARRQLIPFLKGLDLPVNLEDLGLSNLSLHELHEVCQFACQKGSDLYQLPFPVNSNAL
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHH
LEALLGASDNGPVPVESTVTKRIAAS
HHHHHCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.5 {glycerol}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]