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The map label for this gene is tpiA [H]

Identifier: 33863089

GI number: 33863089

Start: 894457

End: 895188

Strand: Reverse

Name: tpiA [H]

Synonym: PMT0817

Alternate gene names: 33863089

Gene position: 895188-894457 (Counterclockwise)

Preceding gene: 33863090

Following gene: 33863088

Centisome position: 37.13

GC content: 53.01

Gene sequence:

>732_bases
GTGCGTAAACCCGTGATCGCTGGCAACTGGAAGATGCATATGACCTGTGCTCAGGCACGGGAGTACACGGCCATGTTCCT
GCCATTAATCGAAGCCACTCCCAATGACCGTCATGTAGTGATTGCCCCACCATTCACCGCTATCTCCTCAATGGCAGAGT
CCGTTGGCGGCACTCGTCTGGAATTGTCAAGCCAGAATGTGCATTGGGAGGATGATGGTGCTTATACCGCAGAGGTTTCG
CCAACGATGCTTCTGGAGCATCAAGTGCGATACGCGATTGTTGGGCATAGTGAACCACGCAAATACTTCAGTGAGAGCGA
TGAACAGATCAACCGTCGTGCTCGTTCAGCCCAAGCGCATGGTCTGATCCCGATTGTCTGCGTGGGTGAAAGTGATGAGC
AGCGTGAACGAGGAGAGGCTGAGCGTGTGATTCGACGACAGGTGGAACAGGGACTCGAAGACACCGATCCAGACAAGCTT
GTGGTTGCTTATGAGCCCATCTGGGCCATTGGCACGGGCAAGACGTGCGAAGCATCGGAGGCGAATCGTATCTGTGGCCT
CATTCGTCGTTGGGTCGGTGCTTCAGAACTAATCATTCAGTACGGCGGTTCGGTCAAACCAGGAAATATTGATGAGTTGA
TGGCAATGAGTGATATCGACGGAGTGTTGGTGGGTGGTGCCTCCCTTGACCCAGAGAGTTTTGCGCGCATTGCCAATTAT
CAAACCAGTTAA

Upstream 100 bases:

>100_bases
AGGCAACCGGGTTCGACTGGGCACAGATGAGGCGGTCTTTGTCGGCAATGCTTCGACAGGGCCGTAAGTTAGCCCCTGCG
GAACTTCAGAGGACCCGAGC

Downstream 100 bases:

>100_bases
ATCTCCTTGGCCTCAGGAATGGGGACAGCGAACGAACGTGATGGGTGTGATTAATATCACGCCCGATTCTTTTAGCGATG
GTGGCAATTATCTAGAGCCT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAESVGGTRLELSSQNVHWEDDGAYTAEVS
PTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAHGLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKL
VVAYEPIWAIGTGKTCEASEANRICGLIRRWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
QTS

Sequences:

>Translated_243_residues
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAESVGGTRLELSSQNVHWEDDGAYTAEVS
PTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAHGLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKL
VVAYEPIWAIGTGKTCEASEANRICGLIRRWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
QTS
>Mature_243_residues
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAESVGGTRLELSSQNVHWEDDGAYTAEVS
PTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAHGLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKL
VVAYEPIWAIGTGKTCEASEANRICGLIRRWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
QTS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=38.4615384615385, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=37.9032258064516, Blast_Score=150, Evalue=9e-37,
Organism=Escherichia coli, GI1790353, Length=246, Percent_Identity=38.2113821138211, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=36.5461847389558, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=34.9593495934959, Blast_Score=139, Evalue=4e-34,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=39.8373983739837, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=39.8373983739837, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=40.8906882591093, Blast_Score=154, Evalue=4e-38,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26831; Mature: 26831

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAESVGGTRL
CCCCEEECCCEEEEEHHHHHHHHHHEEEHHCCCCCCCEEEEECCHHHHHHHHHHCCCCEE
ELSSQNVHWEDDGAYTAEVSPTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAH
EECCCCCEECCCCCEEEECCCHHHHHHCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCC
GLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKLVVAYEPIWAIGTGKTCEASE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCCCH
ANRICGLIRRWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
HHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCC
QTS
CCC
>Mature Secondary Structure
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAESVGGTRL
CCCCEEECCCEEEEEHHHHHHHHHHEEEHHCCCCCCCEEEEECCHHHHHHHHHHCCCCEE
ELSSQNVHWEDDGAYTAEVSPTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAH
EECCCCCEECCCCCEEEECCCHHHHHHCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCC
GLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKLVVAYEPIWAIGTGKTCEASE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCCCH
ANRICGLIRRWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
HHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCC
QTS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA