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The map label for this gene is ispG

Identifier: 33863049

GI number: 33863049

Start: 851774

End: 852994

Strand: Reverse

Name: ispG

Synonym: PMT0777

Alternate gene names: 33863049

Gene position: 852994-851774 (Counterclockwise)

Preceding gene: 33863050

Following gene: 33863048

Centisome position: 35.38

GC content: 49.71

Gene sequence:

>1221_bases
ATGAGTGCAACCATGGCTGAGTATCAATCCAGCACATCCAGGAGATACGACACCCAGATCCACCGACGTGTAACGCGCAC
TGTCAACGTAGGAGGTGTCTTGATCGGTAGTGATCATCCTGTCAGGGTGCAGTCGATGATCAACGAGGACACCCTTGACG
TGGAGGGGGCAACAGCAGGCATACGACGCTTGCATGAAGCTGGTTGTGAAATTGTTCGTCTAACTGTTCCATCTCTCGGT
CATGCCAAGGCCGTCGGCGAGATTTGTCAACGGCTTAGGGAGACCTATCAACCGGTACCCCTTGTGGCTGATGTCCATCA
CAACGGCATGAAAATTGCCCTTGAGGTGGCAAACCATGTCGACAAGGTGAGGATCAATCCTGGCCTTTTTGTGTTTGATA
AAGCTGATCCAGATCGAACCGAGTTCAGCGGCGAAGAGATTGCTTCTATCAGAGAACGCATTGCCGAGAATTTTGAACCG
CTTGTCACTCGTCTCAAGCAGCAAGACAAAGCCCTTCGTATTGGCGTGAATCATGGTTCGCTTGCTGAACGCATGCTCTT
TGCCTATGGAGATACTCCTCTTGGCATGGTTGAGTCAGCAATGGAATTCGTGCGAATTTGCGATTCGCTCGATTTTCATA
ACATCGTGATTTCGATGAAGGCCTCTAGAGCGCCTGTGATGTTGGCGGCTTATCGATTGATGGCTGACACGATGGATCAA
GAGGGTTTCAATTATCCCCTTCATTTGGGCGTAACCGAGGCTGGTGATGGGGATTATGGAAGGATTAAAAGTACAGCGGG
CATCGCAACGCTTTTAGCAGAAGGTCTAGGCGATACCATCCGAGTTTCATTGACCGAGGCGCCTGAGAAAGAAATTCCAG
TTTGTTACTCGATTCTTCAATCCATCGGGCTGCGCAAAACGATGGTCGAGTACATCAGCTGCCCAAGTTGTGGCCGAACA
TTGTTCAACCTTGAAGAGGTTGTTCAGAAAGTGCGCGATTCCACATCCCATCTTGTTGGTTTGGATATTGCGGTAATGGG
TTGCATTGTGAATGGTCCAGGTGAGATGGCAGATGCTGACTATGGTTATGTTGGTAAAGGCCCAGGAGTCATATCCCTCT
ATCGGGGTAGAGATGAGATTCGCAAGGTTTCTGAGTCTGAGGGTGTAGATGCGTTGATCCAGTTGATTAAGGATGACGGT
CGATGGGTGGATCCGCCATGA

Upstream 100 bases:

>100_bases
CCCTCACCAGAGGCGATCTGCTCGAGGTCCGCCAAAGGCTCATACAAGTCAATAGGAACGCTGTTGTGCCAATGTTGGAT
TCATCTAGGCGCCGTCTGCC

Downstream 100 bases:

>100_bases
AGTCGTTATTGTGTGAGATCAGTCCAATTTGGACGCTATGCCCATCTCTAGAAGTCTTCGCTCATTAGGCCGCCAGCGCA
GTTCATGGCTGGTCCTTCTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSATMAEYQSSTSRRYDTQIHRRVTRTVNVGGVLIGSDHPVRVQSMINEDTLDVEGATAGIRRLHEAGCEIVRLTVPSLG
HAKAVGEICQRLRETYQPVPLVADVHHNGMKIALEVANHVDKVRINPGLFVFDKADPDRTEFSGEEIASIRERIAENFEP
LVTRLKQQDKALRIGVNHGSLAERMLFAYGDTPLGMVESAMEFVRICDSLDFHNIVISMKASRAPVMLAAYRLMADTMDQ
EGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLAEGLGDTIRVSLTEAPEKEIPVCYSILQSIGLRKTMVEYISCPSCGRT
LFNLEEVVQKVRDSTSHLVGLDIAVMGCIVNGPGEMADADYGYVGKGPGVISLYRGRDEIRKVSESEGVDALIQLIKDDG
RWVDPP

Sequences:

>Translated_406_residues
MSATMAEYQSSTSRRYDTQIHRRVTRTVNVGGVLIGSDHPVRVQSMINEDTLDVEGATAGIRRLHEAGCEIVRLTVPSLG
HAKAVGEICQRLRETYQPVPLVADVHHNGMKIALEVANHVDKVRINPGLFVFDKADPDRTEFSGEEIASIRERIAENFEP
LVTRLKQQDKALRIGVNHGSLAERMLFAYGDTPLGMVESAMEFVRICDSLDFHNIVISMKASRAPVMLAAYRLMADTMDQ
EGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLAEGLGDTIRVSLTEAPEKEIPVCYSILQSIGLRKTMVEYISCPSCGRT
LFNLEEVVQKVRDSTSHLVGLDIAVMGCIVNGPGEMADADYGYVGKGPGVISLYRGRDEIRKVSESEGVDALIQLIKDDG
RWVDPP
>Mature_405_residues
SATMAEYQSSTSRRYDTQIHRRVTRTVNVGGVLIGSDHPVRVQSMINEDTLDVEGATAGIRRLHEAGCEIVRLTVPSLGH
AKAVGEICQRLRETYQPVPLVADVHHNGMKIALEVANHVDKVRINPGLFVFDKADPDRTEFSGEEIASIRERIAENFEPL
VTRLKQQDKALRIGVNHGSLAERMLFAYGDTPLGMVESAMEFVRICDSLDFHNIVISMKASRAPVMLAAYRLMADTMDQE
GFNYPLHLGVTEAGDGDYGRIKSTAGIATLLAEGLGDTIRVSLTEAPEKEIPVCYSILQSIGLRKTMVEYISCPSCGRTL
FNLEEVVQKVRDSTSHLVGLDIAVMGCIVNGPGEMADADYGYVGKGPGVISLYRGRDEIRKVSESEGVDALIQLIKDDGR
WVDPP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=396, Percent_Identity=36.6161616161616, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_PROMM (Q7V7G9)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894609.1
- ProteinModelPortal:   Q7V7G9
- SMR:   Q7V7G9
- STRING:   Q7V7G9
- GeneID:   1728361
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0777
- NMPDR:   fig|74547.1.peg.776
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   PMAR74547:PMT0777-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 44559; Mature: 44427

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATMAEYQSSTSRRYDTQIHRRVTRTVNVGGVLIGSDHPVRVQSMINEDTLDVEGATAG
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCCHHHHHHCCCCCCCCCHHHH
IRRLHEAGCEIVRLTVPSLGHAKAVGEICQRLRETYQPVPLVADVHHNGMKIALEVANHV
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHHCC
DKVRINPGLFVFDKADPDRTEFSGEEIASIRERIAENFEPLVTRLKQQDKALRIGVNHGS
CEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
LAERMLFAYGDTPLGMVESAMEFVRICDSLDFHNIVISMKASRAPVMLAAYRLMADTMDQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
EGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLAEGLGDTIRVSLTEAPEKEIPVCYSILQ
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
SIGLRKTMVEYISCPSCGRTLFNLEEVVQKVRDSTSHLVGLDIAVMGCIVNGPGEMADAD
HHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHHEEEEECCCCCCCCCC
YGYVGKGPGVISLYRGRDEIRKVSESEGVDALIQLIKDDGRWVDPP
CCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SATMAEYQSSTSRRYDTQIHRRVTRTVNVGGVLIGSDHPVRVQSMINEDTLDVEGATAG
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCCHHHHHHCCCCCCCCCHHHH
IRRLHEAGCEIVRLTVPSLGHAKAVGEICQRLRETYQPVPLVADVHHNGMKIALEVANHV
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHHCC
DKVRINPGLFVFDKADPDRTEFSGEEIASIRERIAENFEPLVTRLKQQDKALRIGVNHGS
CEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
LAERMLFAYGDTPLGMVESAMEFVRICDSLDFHNIVISMKASRAPVMLAAYRLMADTMDQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
EGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLAEGLGDTIRVSLTEAPEKEIPVCYSILQ
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
SIGLRKTMVEYISCPSCGRTLFNLEEVVQKVRDSTSHLVGLDIAVMGCIVNGPGEMADAD
HHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHHEEEEECCCCCCCCCC
YGYVGKGPGVISLYRGRDEIRKVSESEGVDALIQLIKDDGRWVDPP
CCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642