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The map label for this gene is ispE [H]

Identifier: 33862893

GI number: 33862893

Start: 674112

End: 675071

Strand: Reverse

Name: ispE [H]

Synonym: PMT0620

Alternate gene names: 33862893

Gene position: 675071-674112 (Counterclockwise)

Preceding gene: 33862894

Following gene: 33862892

Centisome position: 28.0

GC content: 52.08

Gene sequence:

>960_bases
ATGAGCATATCTAACCCTTCAGCTGCTTCAGAGCATCTGGTTAGCGTCTCGGCGCCAGCCAAGATCAATCTCCATCTTGA
GGTGCTTGGCCTGAGGTCTGATGGTTTTCATGAGCTAGCGATGGTGATGCAGAGCATCGAACTCGCTGATCAACTTCATT
TCCGCAATACGGCTGATGGCAGCATCAGCCTTCGCTGCGATGATTCCAGCCTCAGCACTGCTGGCGACAATTTGATCGTG
CGAGCTGCGCATTTATTACGCGAGCGCTCAGGGTTCTCTGACCTTGGTGCCGCGATTGAATTGCAAAAACGCATCCCAAT
TGGAGCTGGTCTTGCGGGCGGCTCAAGTGATGGTGCAGCAACACTGGTGGGGTTAAACGGTCTCTGGAATCTCAATTTTT
CTCAGGGTCAACTTGAAGGTTTTGCGGCTGAGCTTGGCTCCGACATGCCCTTTTGCCTGGCAGGTGGAAGCCAATTGTGT
TTCGGCCGTGGGGAAAGGTTGGAATCGCTACAAGCGATGAAAGCATCAATGGCCGTGGTGTTGGTGAAGGATCCATCAGT
GAGCGTTTCAACCCCCTGGGCTTATGGACGCTGTAAGGAACTTTTCAGTAGTCGTTATCTTTCAAAGGAATCTGATTTTG
AGCAACGTCGTCAGCAGCTCAGAGAATCTTCTTGGCTGAATCCTTTACGGGCTGATGATCCGCCACCTCTGCGCAACGAC
CTTCAGGAGGTTGTTGCACCCGAAGTTTCGGCTGTGCAAACCACATTGAAGTTGCTCACTGATTTGCCTGGTTCTCTTGC
AGTATCGATGAGTGGATCTGGTCCAAGCTGTTTTGCCCTTTTTGCTGACGTTGCTTCAGCTCAGGCTGCACTTCAGCTCC
AACAGCCTGCCTTCGCCGCAGCTGGTTTAAGCAGTTGGTGCTGCGCGTTCCGCTGTGAAGGCATCAAACTGGAAGCATGA

Upstream 100 bases:

>100_bases
TTGGCATCAGCCTTCAGCAACGCCCCCAAGAACTGTCCCCTGCCACTTGGGTGGAATTGGCGAGGGGTTTGAATCGGGCG
GATCTAGTAGACCCTGAGCC

Downstream 100 bases:

>100_bases
GCGATTCCAAAAACAGCTCTTTCGAGAAGGCAGATGCAGAGTCATCCAACACCTTGGCAGAGACGCCTGCGACACCACGT
AAGGGCCCGCTCAGTTTTCT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSISNPSAASEHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADGSISLRCDDSSLSTAGDNLIV
RAAHLLRERSGFSDLGAAIELQKRIPIGAGLAGGSSDGAATLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLC
FGRGERLESLQAMKASMAVVLVKDPSVSVSTPWAYGRCKELFSSRYLSKESDFEQRRQQLRESSWLNPLRADDPPPLRND
LQEVVAPEVSAVQTTLKLLTDLPGSLAVSMSGSGPSCFALFADVASAQAALQLQQPAFAAAGLSSWCCAFRCEGIKLEA

Sequences:

>Translated_319_residues
MSISNPSAASEHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADGSISLRCDDSSLSTAGDNLIV
RAAHLLRERSGFSDLGAAIELQKRIPIGAGLAGGSSDGAATLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLC
FGRGERLESLQAMKASMAVVLVKDPSVSVSTPWAYGRCKELFSSRYLSKESDFEQRRQQLRESSWLNPLRADDPPPLRND
LQEVVAPEVSAVQTTLKLLTDLPGSLAVSMSGSGPSCFALFADVASAQAALQLQQPAFAAAGLSSWCCAFRCEGIKLEA
>Mature_318_residues
SISNPSAASEHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADGSISLRCDDSSLSTAGDNLIVR
AAHLLRERSGFSDLGAAIELQKRIPIGAGLAGGSSDGAATLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLCF
GRGERLESLQAMKASMAVVLVKDPSVSVSTPWAYGRCKELFSSRYLSKESDFEQRRQQLRESSWLNPLRADDPPPLRNDL
QEVVAPEVSAVQTTLKLLTDLPGSLAVSMSGSGPSCFALFADVASAQAALQLQQPAFAAAGLSSWCCAFRCEGIKLEA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=180, Percent_Identity=37.2222222222222, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 33844; Mature: 33712

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISNPSAASEHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADG
CCCCCCCCHHHHEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
SISLRCDDSSLSTAGDNLIVRAAHLLRERSGFSDLGAAIELQKRIPIGAGLAGGSSDGAA
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCE
TLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLCFGRGERLESLQAMKASMAVV
EEEECCCEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHHHHHHHCEEEE
LVKDPSVSVSTPWAYGRCKELFSSRYLSKESDFEQRRQQLRESSWLNPLRADDPPPLRND
EEECCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LQEVVAPEVSAVQTTLKLLTDLPGSLAVSMSGSGPSCFALFADVASAQAALQLQQPAFAA
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AGLSSWCCAFRCEGIKLEA
HCHHHHHHHHEECCEEECC
>Mature Secondary Structure 
SISNPSAASEHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADG
CCCCCCCHHHHEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
SISLRCDDSSLSTAGDNLIVRAAHLLRERSGFSDLGAAIELQKRIPIGAGLAGGSSDGAA
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCE
TLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLCFGRGERLESLQAMKASMAVV
EEEECCCEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHHHHHHHCEEEE
LVKDPSVSVSTPWAYGRCKELFSSRYLSKESDFEQRRQQLRESSWLNPLRADDPPPLRND
EEECCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LQEVVAPEVSAVQTTLKLLTDLPGSLAVSMSGSGPSCFALFADVASAQAALQLQQPAFAA
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AGLSSWCCAFRCEGIKLEA
HCHHHHHHHHEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA