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The map label for this gene is ksgA [H]

Identifier: 33862894

GI number: 33862894

Start: 675068

End: 675910

Strand: Reverse

Name: ksgA [H]

Synonym: PMT0621

Alternate gene names: 33862894

Gene position: 675910-675068 (Counterclockwise)

Preceding gene: 33862896

Following gene: 33862893

Centisome position: 28.04

GC content: 53.86

Gene sequence:

>843_bases
ATGGCCTTTTCCGGACATCACGCTCGCAAGCGCTTTGCTCAACACTGGCTAATCGATGCCGCTGTGTTGACTCAGATTCT
CGATGCAGCTGATGTTCAACCAGATGATCGTCTGCTGGAGGTAGGTCCTGGTCGTGGAGCACTCACTGAAAGGTTGCTGG
CCTCTTCTGCCTCTGCTGTTCATGCTGTTGAACTTGATCGCGATCTGGTCTCTGGGTTGAAGCAGCGTTTTGCGGATCAA
GCCCGCTTCAGCTTGCAAGAAGGTGATGTGTTGTCTGTTCCATTGACTCTTGCTGATGGCCGTGCTGCGACCAAAGTGGT
GGCCAATATTCCCTATAACATCACCGGTCCTCTGCTTGAGCGTCTTCTCGGTCGCTTGGATCGACCTGTTGATCATCCCT
ATCAACGCCTTGTGCTTCTTTTGCAAAAGGAAGTCGCTCAACGGATTCGTGCCTTGCCCGGGCAGAGCTGTTTTAGTGCT
CTAAGCGTGCGCCTGCAGTTGCTCGCTCGTTGCACAACTGTTTGCCCAGTGCCGCCGCGCTCTTTTAAACCGCCGCCGAA
AGTTCATTCTGAGGTCATCTTGATTGAACCTCTTGCTCCTGAACAGCGCCTTGAGCCACTGCTCGCCAAACGTGTTGAGT
CTTTGCTGCGTCAGGCTTTTTTGGCCAGGCGCAAGATGCTGCGCAACACTCTTGCCAAGGTTCTTCCTGCTGCTGAACTG
AATGCTCTTGCTGATGATCTTGGCATCAGCCTTCAGCAACGCCCCCAAGAACTGTCCCCTGCCACTTGGGTGGAATTGGC
GAGGGGTTTGAATCGGGCGGATCTAGTAGACCCTGAGCCATGA

Upstream 100 bases:

>100_bases
TGATTGGAACAGCGAGTTTGACTCCGAGTTTTGTCTGCAGCTTTTTTTGATCTTCAATCAATTGCGTCGCCCTCTATACA
GATTGGTGCAAAGTTGAGGG

Downstream 100 bases:

>100_bases
GCATATCTAACCCTTCAGCTGCTTCAGAGCATCTGGTTAGCGTCTCGGCGCCAGCCAAGATCAATCTCCATCTTGAGGTG
CTTGGCCTGAGGTCTGATGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAVHAVELDRDLVSGLKQRFADQ
ARFSLQEGDVLSVPLTLADGRAATKVVANIPYNITGPLLERLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSA
LSVRLQLLARCTTVCPVPPRSFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP

Sequences:

>Translated_280_residues
MAFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAVHAVELDRDLVSGLKQRFADQ
ARFSLQEGDVLSVPLTLADGRAATKVVANIPYNITGPLLERLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSA
LSVRLQLLARCTTVCPVPPRSFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP
>Mature_279_residues
AFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAVHAVELDRDLVSGLKQRFADQA
RFSLQEGDVLSVPLTLADGRAATKVVANIPYNITGPLLERLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSAL
SVRLQLLARCTTVCPVPPRSFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAELN
ALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=218, Percent_Identity=36.697247706422, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=27.7966101694915, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1786236, Length=273, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=35.7798165137615, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=27.6094276094276, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=279, Percent_Identity=29.0322580645161, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=256, Percent_Identity=35.546875, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21357273, Length=291, Percent_Identity=27.4914089347079, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30883; Mature: 30752

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHH
HAVELDRDLVSGLKQRFADQARFSLQEGDVLSVPLTLADGRAATKVVANIPYNITGPLLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHH
RLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSALSVRLQLLARCTTVCPVPPR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP
HHHHHHHCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHH
HAVELDRDLVSGLKQRFADQARFSLQEGDVLSVPLTLADGRAATKVVANIPYNITGPLLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHH
RLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSALSVRLQLLARCTTVCPVPPR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP
HHHHHHHCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA