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The map label for this gene is gshR [H]

Identifier: 33862743

GI number: 33862743

Start: 521796

End: 523157

Strand: Reverse

Name: gshR [H]

Synonym: PMT0470

Alternate gene names: 33862743

Gene position: 523157-521796 (Counterclockwise)

Preceding gene: 33862746

Following gene: 33862741

Centisome position: 21.7

GC content: 53.6

Gene sequence:

>1362_bases
TTGGAGCATCCTTTTGATCTGATTGTGCTTGGTGCTGGCTCTGGTGGACTAGCCGCTGCTAAGCGAGCCGCAAGCCATGG
TGCCAAGGTCGCCATCGTTGAAGGTGACCGTGTTGGAGGGACCTGCGTCATTCGCGGCTGTGTACCTAAGAAGCTGTTGG
TGTACGGATCTCTTTATCGGGAACAGCTTGAAGTTGCCCCCAGTTTTGGAGTTGAGTTGAGTGATGCACAGATCAATGCG
GGGGTGTTGCTAGCCAATGTGCGTCAGGAAGTGGATCGTCTCAATACTCTTCACATTGACCTGCTAGCCAAAAACGGTGT
TGAGCTTGTGACCGGATGGGGGAGTTTTACGAGCCCAAACAGCGTGGCTGTCTCAAGGAACGGAGCCATCGATGCTTCAC
AGGAACTTCACGGCGATCACATTCTGATCGCGGTCGGTGGGCGTCCCCATCGTCCTGATATCCCAGGTGCGGAGTTGGGA
TGGGTTAGCGATGACATGTTCATGCAAAGCAGCTTCCCCGACCGAGTGGTGGTGGTGGGGGCTGGATTTATTGCCTGTGA
ATTTGCCTGCATCCTCCATGGCCTTGGCGTCGAGGTCATCCAACTGGTGCGAGGGGATCACTTGCTGCGTGGCTTTGATC
GGGAGCTCTCCACTGCAGTACAGGATGGAATGCAGGAGAAAGGCATTGATCTTCGTTTTGGAGAAAACCCAGCCGCTCTA
AAAGGCCAACCTGGCGATCTCGTCTTGTCAACCCAGAGTGGGGAACGCTTCTCTTGTGGGGGCGTTCTTCTTGCTACAGG
ACGACGACCTTTTTTGCAAGGACTCAACCTTGACGCTGCTGGGGTTGTTTTAGAAGGTCGACGTATTTCAGTGGATGCCA
ACCAATCCACAAATATCCCTCACATCTTTGCTGTTGGTGATGTAACAGACCGCATCAACCTCACCCCTGTGGCAATCGAT
GAGGGCAGGGCATTCGCCGACAGTGTTTTTGGACAGAAACCCCGCCAGGTCAATCATGACCTCGTTGCCAGTGCTGTCTT
CAGCCAACCAGAGCTAGCCACAGTTGGGCTCACTGAAGAGCAGTCGATTGATCGCTATGGCAAAGATCAAGTCAAGGTCT
ACCGCGCCCGCTTCCGATCCATGGCTCAGGCCTTGCCGAAGCGAGGGCCCCGTTGTCTTCTCAAGTTAATTGTGGCACGC
AACACTGATCGAATCCTTGGTTGTCACATGGTGGGGGAACATGCTGCCGAAATCATTCAGATGGCAGCGATTGCACTTGG
AATGGGTGCTACAAAGGCAGACTTTGATCGCACCATGGCATTACATCCCACCGTTTCTGAGGAGTTTGTCACCATGACTT
AG

Upstream 100 bases:

>100_bases
GAGTCATGACAGGTTTACTGATCATTGTCGTCACGATCCCTGAGTAATCCTGGCGTTGCTTTCTTAAGGAGTTGACAATG
GATGCAGTGATCCTCGTCTC

Downstream 100 bases:

>100_bases
GGAGTATTACTGAAGATAAATTCTATCCATCCTTTTAGTCGATCACTTGCGCCATTTGAGTCGTCCAGGCAAGGCCTTAG
GGGATCTGTTACGGCTCGGC

Product: glutathione reductase (NADPH)

Products: NA

Alternate protein names: GR; GRase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MEHPFDLIVLGAGSGGLAAAKRAASHGAKVAIVEGDRVGGTCVIRGCVPKKLLVYGSLYREQLEVAPSFGVELSDAQINA
GVLLANVRQEVDRLNTLHIDLLAKNGVELVTGWGSFTSPNSVAVSRNGAIDASQELHGDHILIAVGGRPHRPDIPGAELG
WVSDDMFMQSSFPDRVVVVGAGFIACEFACILHGLGVEVIQLVRGDHLLRGFDRELSTAVQDGMQEKGIDLRFGENPAAL
KGQPGDLVLSTQSGERFSCGGVLLATGRRPFLQGLNLDAAGVVLEGRRISVDANQSTNIPHIFAVGDVTDRINLTPVAID
EGRAFADSVFGQKPRQVNHDLVASAVFSQPELATVGLTEEQSIDRYGKDQVKVYRARFRSMAQALPKRGPRCLLKLIVAR
NTDRILGCHMVGEHAAEIIQMAAIALGMGATKADFDRTMALHPTVSEEFVTMT

Sequences:

>Translated_453_residues
MEHPFDLIVLGAGSGGLAAAKRAASHGAKVAIVEGDRVGGTCVIRGCVPKKLLVYGSLYREQLEVAPSFGVELSDAQINA
GVLLANVRQEVDRLNTLHIDLLAKNGVELVTGWGSFTSPNSVAVSRNGAIDASQELHGDHILIAVGGRPHRPDIPGAELG
WVSDDMFMQSSFPDRVVVVGAGFIACEFACILHGLGVEVIQLVRGDHLLRGFDRELSTAVQDGMQEKGIDLRFGENPAAL
KGQPGDLVLSTQSGERFSCGGVLLATGRRPFLQGLNLDAAGVVLEGRRISVDANQSTNIPHIFAVGDVTDRINLTPVAID
EGRAFADSVFGQKPRQVNHDLVASAVFSQPELATVGLTEEQSIDRYGKDQVKVYRARFRSMAQALPKRGPRCLLKLIVAR
NTDRILGCHMVGEHAAEIIQMAAIALGMGATKADFDRTMALHPTVSEEFVTMT
>Mature_453_residues
MEHPFDLIVLGAGSGGLAAAKRAASHGAKVAIVEGDRVGGTCVIRGCVPKKLLVYGSLYREQLEVAPSFGVELSDAQINA
GVLLANVRQEVDRLNTLHIDLLAKNGVELVTGWGSFTSPNSVAVSRNGAIDASQELHGDHILIAVGGRPHRPDIPGAELG
WVSDDMFMQSSFPDRVVVVGAGFIACEFACILHGLGVEVIQLVRGDHLLRGFDRELSTAVQDGMQEKGIDLRFGENPAAL
KGQPGDLVLSTQSGERFSCGGVLLATGRRPFLQGLNLDAAGVVLEGRRISVDANQSTNIPHIFAVGDVTDRINLTPVAID
EGRAFADSVFGQKPRQVNHDLVASAVFSQPELATVGLTEEQSIDRYGKDQVKVYRARFRSMAQALPKRGPRCLLKLIVAR
NTDRILGCHMVGEHAAEIIQMAAIALGMGATKADFDRTMALHPTVSEEFVTMT

Specific function: Maintains high levels of reduced glutathione in the cytosol [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=38.0645161290323, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=35, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI33519430, Length=461, Percent_Identity=30.8026030368764, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI33519428, Length=461, Percent_Identity=30.8026030368764, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI33519426, Length=461, Percent_Identity=30.8026030368764, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI148277071, Length=461, Percent_Identity=30.8026030368764, Blast_Score=219, Evalue=6e-57,
Organism=Homo sapiens, GI148277065, Length=461, Percent_Identity=30.8026030368764, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI291045266, Length=462, Percent_Identity=31.6017316017316, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=30.7359307359307, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI291045268, Length=461, Percent_Identity=29.7180043383948, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=40.3669724770642, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI87082354, Length=457, Percent_Identity=28.0087527352298, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=27.5938189845475, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=25.0549450549451, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=453, Percent_Identity=38.6313465783664, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71983419, Length=453, Percent_Identity=38.6313465783664, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=34.3949044585987, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71982272, Length=484, Percent_Identity=32.8512396694215, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=31.5904139433551, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=36.6379310344828, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6321091, Length=468, Percent_Identity=29.9145299145299, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.9230769230769, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=37.5527426160338, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24640553, Length=472, Percent_Identity=35.5932203389831, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=35.5932203389831, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24640551, Length=472, Percent_Identity=35.8050847457627, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=31.317494600432, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006324
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.7 [H]

Molecular weight: Translated: 48407; Mature: 48407

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHPFDLIVLGAGSGGLAAAKRAASHGAKVAIVEGDRVGGTCVIRGCVPKKLLVYGSLYR
CCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHH
EQLEVAPSFGVELSDAQINAGVLLANVRQEVDRLNTLHIDLLAKNGVELVTGWGSFTSPN
HHHHCCCCCCCEECCCCCCCCEEEHHHHHHHHHHCEEEEEEEECCCEEEEECCCCCCCCC
SVAVSRNGAIDASQELHGDHILIAVGGRPHRPDIPGAELGWVSDDMFMQSSFPDRVVVVG
CEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEE
AGFIACEFACILHGLGVEVIQLVRGDHLLRGFDRELSTAVQDGMQEKGIDLRFGENPAAL
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCC
KGQPGDLVLSTQSGERFSCGGVLLATGRRPFLQGLNLDAAGVVLEGRRISVDANQSTNIP
CCCCCCEEEECCCCCEEECCEEEEECCCCHHHHCCCCCCCCEEEECEEEEEECCCCCCCC
HIFAVGDVTDRINLTPVAIDEGRAFADSVFGQKPRQVNHDLVASAVFSQPELATVGLTEE
EEEEECCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCH
QSIDRYGKDQVKVYRARFRSMAQALPKRGPRCLLKLIVARNTDRILGCHMVGEHAAEIIQ
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
MAAIALGMGATKADFDRTMALHPTVSEEFVTMT
HHHHHHCCCCCCCCCCCEEEECCCCCCCEEECC
>Mature Secondary Structure
MEHPFDLIVLGAGSGGLAAAKRAASHGAKVAIVEGDRVGGTCVIRGCVPKKLLVYGSLYR
CCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHH
EQLEVAPSFGVELSDAQINAGVLLANVRQEVDRLNTLHIDLLAKNGVELVTGWGSFTSPN
HHHHCCCCCCCEECCCCCCCCEEEHHHHHHHHHHCEEEEEEEECCCEEEEECCCCCCCCC
SVAVSRNGAIDASQELHGDHILIAVGGRPHRPDIPGAELGWVSDDMFMQSSFPDRVVVVG
CEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEE
AGFIACEFACILHGLGVEVIQLVRGDHLLRGFDRELSTAVQDGMQEKGIDLRFGENPAAL
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCC
KGQPGDLVLSTQSGERFSCGGVLLATGRRPFLQGLNLDAAGVVLEGRRISVDANQSTNIP
CCCCCCEEEECCCCCEEECCEEEEECCCCHHHHCCCCCCCCEEEECEEEEEECCCCCCCC
HIFAVGDVTDRINLTPVAIDEGRAFADSVFGQKPRQVNHDLVASAVFSQPELATVGLTEE
EEEEECCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCH
QSIDRYGKDQVKVYRARFRSMAQALPKRGPRCLLKLIVARNTDRILGCHMVGEHAAEIIQ
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
MAAIALGMGATKADFDRTMALHPTVSEEFVTMT
HHHHHHCCCCCCCCCCCEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559423; 11759840 [H]