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The map label for this gene is yafV [C]

Identifier: 33862668

GI number: 33862668

Start: 443464

End: 444285

Strand: Reverse

Name: yafV [C]

Synonym: PMT0395

Alternate gene names: 33862668

Gene position: 444285-443464 (Counterclockwise)

Preceding gene: 33862669

Following gene: 33862667

Centisome position: 18.43

GC content: 55.84

Gene sequence:

>822_bases
GTGACCGACTTTCTGGCAGCCGCTTTGCAGCTGACGAGCACCTCGGATCCAGAGCTCAATTTTGCTGCTGCCGAGGAGCA
GATCGAACTGGCTGCCCGCCGTGGGGCGGAATTGGTGGGATTACCTGAAAATTTTGCCTTTATGGGTGATGACGAGCGAC
GTCTGGAGCTGGCACAAGACTTAGCTGAGCAATGCAGCCGCTTTCTGGTCACCATGGCAAGGCGCTACCAGGTGGTGCTT
CTGGGCGGGGGCTTCCCTGTACCTTCAGGAGATAGCAATCACACGGTCAACAGAGCCGAATTGGTCGGTCGTGATGGTCA
GCTGCTAGCGAGATACGACAAGATTCATCTCTTCGATGTGGATCTACCTGAAGGCAACACCTATCAGGAGTCGGCCACGA
CTACGTCTGGTCGTGAACTACCCCCGGTCGTGGATGTGCCAGGACTATGTCGAGTGGGACTATCCATCTGCTACGACGTG
CGCTTCCCTGAGCTCTATCGCCATCTCGTCAGTGCTGGTGCCGAGTTATTGATGATTCCAGCAGCATTCACGGCTTTCAC
CGGAAAAGATCACTGGCAGGTCTTACTTCAAGCCAGGGCCATCGAAAACACCTCCTATGTGCTTGCACCTGCCCAGACAG
GTCGTCATTACAGCCGTCGACAGAGCCATGGACATGCCATGGTGATTGATCCCTGGGGAACCGTCTTGGCTGATGCAGGA
GTGAGTCAGGGTGCAGCCATTGCTCCTGTGGATAACTCGCACATTGGTCGCATTCGCGCGCAAATGCCAAGCCTCCAACA
CCGGCAGTCAGCACTGTTCTGA

Upstream 100 bases:

>100_bases
GGATCGGCTTTCTGTGGTTCCTGTTCAGGTCAAACCCGGAGTCCTTTGTGCCTCTTGAAACCTCACTGCGATCTAAAGTT
TCGCTCTCTGTTTCAACCCT

Downstream 100 bases:

>100_bases
GCCAATGGCCTCGGCTCCATCCCGCCCTCTCATCCTGCTGGTGGTTGGTGCCTTGCAGATCCCTTTGGTTTTGGCAGCGT
TGCCGGCCAGAGCGGCAAGT

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTDFLAAALQLTSTSDPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQDLAEQCSRFLVTMARRYQVVL
LGGGFPVPSGDSNHTVNRAELVGRDGQLLARYDKIHLFDVDLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDV
RFPELYRHLVSAGAELLMIPAAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQSALF

Sequences:

>Translated_273_residues
MTDFLAAALQLTSTSDPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQDLAEQCSRFLVTMARRYQVVL
LGGGFPVPSGDSNHTVNRAELVGRDGQLLARYDKIHLFDVDLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDV
RFPELYRHLVSAGAELLMIPAAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQSALF
>Mature_272_residues
TDFLAAALQLTSTSDPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQDLAEQCSRFLVTMARRYQVVLL
GGGFPVPSGDSNHTVNRAELVGRDGQLLARYDKIHLFDVDLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDVR
FPELYRHLVSAGAELLMIPAAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAGV
SQGAAIAPVDNSHIGRIRAQMPSLQHRQSALF

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=273, Percent_Identity=41.025641025641, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI297632348, Length=273, Percent_Identity=41.025641025641, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI5031947, Length=273, Percent_Identity=41.025641025641, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI9910460, Length=273, Percent_Identity=36.2637362637363, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=38.3419689119171, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17556280, Length=271, Percent_Identity=42.8044280442804, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6322335, Length=303, Percent_Identity=33.6633663366337, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=31.3868613138686, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17933642, Length=266, Percent_Identity=36.8421052631579, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=31.2267657992565, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29868; Mature: 29737

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLAAALQLTSTSDPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQD
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEECCCCHHHHHHHHH
LAEQCSRFLVTMARRYQVVLLGGGFPVPSGDSNHTVNRAELVGRDGQLLARYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEECEEEEEEE
DLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDVRFPELYRHLVSAGAELLMIP
ECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHHCCCCEEEEC
AAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
HHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEECCCHHHHHHCC
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQSALF
CCCCCEEECCCCCHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TDFLAAALQLTSTSDPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQD
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEECCCCHHHHHHHHH
LAEQCSRFLVTMARRYQVVLLGGGFPVPSGDSNHTVNRAELVGRDGQLLARYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEECEEEEEEE
DLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDVRFPELYRHLVSAGAELLMIP
ECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHHCCCCEEEEC
AAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
HHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEECCCHHHHHHCC
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQSALF
CCCCCEEECCCCCHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]