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The map label for this gene is pheS

Identifier: 33862640

GI number: 33862640

Start: 413385

End: 414392

Strand: Direct

Name: pheS

Synonym: PMT0367

Alternate gene names: 33862640

Gene position: 413385-414392 (Clockwise)

Preceding gene: 33862638

Following gene: 33862641

Centisome position: 17.15

GC content: 56.65

Gene sequence:

>1008_bases
GTGAGCGCCACTATTTCCTTGCAGCAGCTCACCGACCAGCTTGATGCCCTCGAGGCTGAGGCGGTGTCTGCTATCGCTGC
TGCCGCAGATGCGGATGCCTTGGAGCAATTGCGGGTGAGTCTGTTGGGCAAGAAAGGGCGCTTGTCTGGTGTTCTCGGGG
CGATGGGCAAGCTCCCTAGTGAAGAGCGACCGCTTGTTGGTCAGCGCGCCAATGTGCTTAAAACACAGGTGCAAAACCTG
CTAGGGGAACGGTTGCAGGTGGTGAAAGCGGCAGCTTTGGCTGATCGGCTGCTGCAGGAAACGATTGATGTCACTGCGCC
TGCCAGTGGCATTCCTGTGGGCCATCGCCACCCTTTGATTACAACAACTGAGCAGATCGTGGATCTGTTTTCTGGTTTGG
GCTATCGGGTGGCTGAAGGCCCGGAGGTGGAGACCGATCACTACAACTTTTCCGCCTTGAATATTCCCGAGGACCATCCC
GCTCGAGACATGCAGGACACGTTCTATCTGGGCGGCAATCTGCTTCTGCGTACCCACACCTCGCCTGTGCAGATTCGCTA
TCTAGAAGAGAATCCGCCGCCAGTAAGGATTGTGGCGCCAGGCCGGGTTTATCGGCGTGATGCAGTGGATGCGACCCATT
CGCCTGTCTTTCATCAGGTTGAGGTGTTGGCCATTGATGAAGGCCTTGATTTCAGCCATCTGCGCGGCACGGTGATGGCT
TTCCTCAAGGCATTCTTTGGCGATATGCCGGTGAGATTTCGGGCTAGTTATTTCCCCTTTACCGAACCATCGGCAGAGGT
GGATGTGCAGTGGCGTGGGCGTTGGCTGGAGGTGATGGGTTGCGGCATGGTTGATCCAGCAGTGTTAGAGGGCCTGGGGC
TTGATCCAGAGCGCTGGAGTGGGTTTGCGGCAGGCCTTGGTGTGGAACGCTTCTGCATGGTCCGTCATGGCATCGACGAC
ATTCGCAGGCTTTATACGAGTGATTTGCGCTTCCTTGAGCAGTTTTGA

Upstream 100 bases:

>100_bases
ATTGCTAATCAGAATTTGCAACGGAGTCATCTCCTCGTATCCACTGCGGGGAACTTAATCATGATGACTACCTAAAGTCA
GTGGCTGCGATCTTTTTTCA

Downstream 100 bases:

>100_bases
GTCGATAGCCGATCGTGTGCTGGATGAAGCATCGCCGTAGTGCATCATCCAAAAACAGCTGAATCCCCATAAAGTGAGCT
CGTTCTTTGCTTCATTCGAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPSEERPLVGQRANVLKTQVQNL
LGERLQVVKAAALADRLLQETIDVTAPASGIPVGHRHPLITTTEQIVDLFSGLGYRVAEGPEVETDHYNFSALNIPEDHP
ARDMQDTFYLGGNLLLRTHTSPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWSGFAAGLGVERFCMVRHGIDD
IRRLYTSDLRFLEQF

Sequences:

>Translated_335_residues
MSATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPSEERPLVGQRANVLKTQVQNL
LGERLQVVKAAALADRLLQETIDVTAPASGIPVGHRHPLITTTEQIVDLFSGLGYRVAEGPEVETDHYNFSALNIPEDHP
ARDMQDTFYLGGNLLLRTHTSPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWSGFAAGLGVERFCMVRHGIDD
IRRLYTSDLRFLEQF
>Mature_334_residues
SATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPSEERPLVGQRANVLKTQVQNLL
GERLQVVKAAALADRLLQETIDVTAPASGIPVGHRHPLITTTEQIVDLFSGLGYRVAEGPEVETDHYNFSALNIPEDHPA
RDMQDTFYLGGNLLLRTHTSPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMAF
LKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWSGFAAGLGVERFCMVRHGIDDI
RRLYTSDLRFLEQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=260, Percent_Identity=34.6153846153846, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI5729820, Length=230, Percent_Identity=34.3478260869565, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=51.6923076923077, Blast_Score=330, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=34.469696969697, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=262, Percent_Identity=32.0610687022901, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=262, Percent_Identity=32.0610687022901, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321087, Length=276, Percent_Identity=32.9710144927536, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=88, Percent_Identity=44.3181818181818, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137424, Length=278, Percent_Identity=29.8561151079137, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18858079, Length=295, Percent_Identity=30.1694915254237, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PROMM (Q7TV42)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894200.1
- ProteinModelPortal:   Q7TV42
- SMR:   Q7TV42
- STRING:   Q7TV42
- GeneID:   1729305
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0367
- NMPDR:   fig|74547.1.peg.367
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   PMAR74547:PMT0367-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 36956; Mature: 36824

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPS
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
EERPLVGQRANVLKTQVQNLLGERLQVVKAAALADRLLQETIDVTAPASGIPVGHRHPLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
TTTEQIVDLFSGLGYRVAEGPEVETDHYNFSALNIPEDHPARDMQDTFYLGGNLLLRTHT
ECHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCHHEEECCEEEEEECC
SPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
CCEEEEEECCCCCCEEEECCCHHHHHHCCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHH
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWS
HHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCHHHHC
GFAAGLGVERFCMVRHGIDDIRRLYTSDLRFLEQF
CHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPS
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
EERPLVGQRANVLKTQVQNLLGERLQVVKAAALADRLLQETIDVTAPASGIPVGHRHPLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
TTTEQIVDLFSGLGYRVAEGPEVETDHYNFSALNIPEDHPARDMQDTFYLGGNLLLRTHT
ECHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCHHEEECCEEEEEECC
SPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
CCEEEEEECCCCCCEEEECCCHHHHHHCCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHH
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWS
HHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCHHHHC
GFAAGLGVERFCMVRHGIDDIRRLYTSDLRFLEQF
CHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642